Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Abstract

The white Nelumbo nucifera petals aqueous extraction (NAE) was prominent in phytochemical content, antioxidant activity, and enhanced rat sperm viability induced by FeSO4, a heavy metal. Mancozeb (MZ) contains heavy metals and is widely used for fungal control in agriculture and industry. It induces oxidative stress and causes of spermatogenesis and reproductive organs’ abnormalities in both humans and animals. The aims of the present study were to investigate the effects of white Nelumbo nucifera petals aqueous extraction (WNAE) on sperm quality in cattle sperm induced by MZ. Moreover, this study investigated phytochemical compounds by liquid chromatography-mass spectrometry. A protein profile related to sperm quality with SDS-page and sperm energy preservation for each treatment was determined. The results found nine phytochemical compounds, in which quercetin-3-O-arabinoglycoside was a major flavonoid that was found in the WNAE. MZ induced free radicals in cells, leading to LPO and protein oxidation, while decreasing sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The cattle sperm found four proteins related to sperm quality including MWs of 17, 31, 34, and 55 kDa. The WNAE effectively increased energy preservation, sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The WNAE enhanced sperm qualities by reducing oxidative stress. It might be suggested that WNAE has benefits for sperm preservation which may be used to guard against toxicity in animals or humans exposed to MZ contaminants.

Neuroprotective Effect of White Nelumbo nucifera Gaertn. Petal Tea in Rats Poisoned with Mancozeb

“…The fine powder of white N. nucifera petals was extracted with hot water at 75-80 • C (1 mg/mL) for 3-5 min. Then, the solutions were filtered and dried by lyophilization with a 12.5% yield [12] and kept at −20 • C until further use in different doses.…”

“…Moreover, histopathological changes in the hippocampus of rats poisoned with chlorpyrifos and cypermethrin, especially pyramidal cells in CA1 and CA3 regions, were induced by stimulating ChE activity and impairing neuron conductivity [53]. Additionally, a recent study showed that white N. nucifera petal extract at a dose of 1.10 mg/kg bw [12] had no toxic effect on the hippocampus structure and learning-memory after oral administration for 30 consecutive days [57]. In view of these and the discussion of the cognitive behavior changes above, further research on the connection between histopathological changes and cognitive behaviors would be helpful in the evaluation of mancozeb and white N. nucifera petal extract effects on brain function.…”

“…The petals of N. nucifera are rich in gallic acid, as well as flavonoids, including luteolin, quercetin, naringenin, isorhamnetin, cyanidin, and delphinidin [10]. The white N. nucifera petals have high contents of phenols and tannins, both of which are strong antioxidants and have been shown to decrease the toxicity of mancozeb in Charolais cattle sperm [11,12]. However, there is limited data concerning its effect on the neurological system.…”

Inclusion of Spirulina platensis and Salvia verbenaca extracts to boost semen quality and fertilization ability in sheep

“…To avoid such negative effects, the inclusion of antioxidants in extenders can minimize the negative effect of ROS and maintain sperm quality after liquid storage (Alenezy et al, 2019). Recently, the protective effect of natural antioxidants on sperm has been largely discussed (Ajuogu et al, 2020; Allai et al, 2018; Laoung‐on et al, 2022; Ros‐Santaella & Pintus, 2021). However, a large list of those studies focused mainly on in vitro assessments, while the information about the fertility results using the natural extracts remains limited.…”

“…Recently, the protective effect of natural antioxidants on sperm has been largely discussed (Ajuogu et al, 2020;Allai et al, 2018;Laoung-on et al, 2022;Ros-Santaella & Pintus, 2021). However, a large list of those studies focused mainly on in vitro assessments, while the information about the fertility results using the natural extracts remains limited.…”