Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

Raseona, A. M.; Ajao, O. A.; Nethengwe, L. D.; Madzhie, L. R.; Nedambale, T. L.; Barry, D.M.

doi:10.4314/sajas.v47i1.8

Cited by 6 publications

(4 citation statements)

References 22 publications

(25 reference statements)

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“…To the best of our knowledge, very little information is available about the correlation between the extender type, BBE levels, and enzymatic activities in ram seminal plasma. Hence, the transaminase activities (AST and ALT) in extenders are a good indicator of semen quality because they measure sperm membrane stability [ 62 ]. For instance, ALT, AST, and ALP are fundamental for metabolic procedures that provide the energy necessary for sperm motility, viability, and fertility [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

The Supplementation of Bee Bread Methanolic Extract to Egg Yolk or Soybean Lecithin Extenders Can Improve the Quality of Cryopreserved Ram Semen

Sharaf

Khalil

Khalifa

et al. 2022

Cells

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Bee bread has numerous nutritional benefits and bioactive compounds. Other bee byproducts have been used as extender additives to improve semen cryopreservation. Here, we examined the effects of supplementing egg yolk extender (EYE) or soybean lecithin extender (SBLE) with bee bread extract (BBE) on the quality of cryopreserved ram semen. Semen was collected from five adult Rahmani rams once a week for 7 weeks. EYE and SBLE were supplemented with BBE. Antioxidant capacity and total phenolic compound, total flavonoid compound, and total soluble carbohydrate levels of BBE were measured. Sperm characteristics, including progressive motility, viability, abnormalities, membrane integrity, and acrosome integrity, were analyzed after equilibration, thawing, and thawing followed by a 2-h incubation. The total antioxidant capacity and malondialdehyde, hydrogen peroxide, aspartate transaminase, alanine transaminase, alkaline phosphatase, and total acid phosphatase levels in extenders were determined after thawing. Sperm apoptosis was analyzed using annexin V assays. SBLE was more effective than EYE for cryopreserving ram semen. Extender supplementation with BBE improved ram semen quality during freezing in a concentration-dependent pattern. Motility, vitality, and membrane integrity were particularly enhanced in BBE-treated semen. Additionally, BBE promoted antioxidant and enzymatic activities and reduced apoptosis in semen. Thus, extender supplementation with BBE improved sperm cryopreservation.

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Section: Discussionmentioning

confidence: 99%

The Supplementation of Bee Bread Methanolic Extract to Egg Yolk or Soybean Lecithin Extenders Can Improve the Quality of Cryopreserved Ram Semen

Sharaf

Khalil

Khalifa

et al. 2022

Cells

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“…Semen storage at 25⁰C or 5⁰C for 30 minutes did not significantly affect spermatozoa viability but negatively affected spermatozoa motility (Alkali et al, 2022). Cell damage can cause spermatozoa death and be a source of toxicity for live spermatozoa (Raseona et al, 2017).…”

Section: Velocity Of Spermatozoa In Various Diluentsmentioning

confidence: 99%

“…Storage of spermatozoa at ambient temperature causes an increase in the rate of cell metabolism resulting in a deficit of energy sources, ATP, and accumulation of lactic acid and AAAO enzymes, so that the survival life of spermatozoa at ambient temperature (25-27°C) is short (Maulana, 2016;Raseona et al, 2017). The energy deficit causes an ATP deficit which affects the movement of the spermatozoa's tail so that the spermatozoa's motility decreases.…”

Section: Velocity Of Spermatozoa In Various Diluentsmentioning

confidence: 99%

Study Motility of Ongole Crossbred Semen with Soybean-Based Diluents Stored at Ambient Temperature (24-27 0C)

Ratnawati¹,

Ciptadi

Rahayu

et al. 2023

JIIP

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Artificial insemination with fresh semen is an alternative that can be done if there is a limitation of liquid nitrogen, bull, and facilities for processing liquid and frozen semen(costly). This study aimed to determine the optimal diluent formula based on CEP-3 (modified/m) and aqueous soybean extract (ASE) to support spermatozoa motility at ambient temperature storage. The research design was completely randomized, with eight treatments and six replications. The diluent formula treatments were as follows: T1 (CEP-3(m)), T2 (CEP-3(m)+10% Egg Yolk/EY), T3 (CEP-3(m)+ASE 1%), T4 (CEP-3 (m)+ASE 2%), T5 (CEP-3(m)+ASE 3%), T6 (CEP-3(m)+ASE 4%), T7 (CEP-3(m)+ASE 5%), and T8 (CEP-3(m)+ASE 6%). The parameters were measured, i.e., the motility of spermatozoa and itskinetic parameters.Data were analyzed using ANOVA with SPSS 16 program. The result showed that the progressive motility of spermatozoa at day one was more than 50% in diluents CEP-3(m)+ASE 1-2%. Diluent CEP-3(m) and aqueous soybean extract 1-2% can support spermatozoa's motility and kinetic parameters during storage at ambient temperature, including progressive motility, motility, motility, velocity, and BCF.

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“…An optimal diluent composition is expected to protect spermatozoa from damage caused by cooling, such as cryodamage or cryoinjury [6][7][8]. Liquid semen is considered successful if the decrease in motility (M) and metabolic activity due to cooling can be reversible [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of modified CEP-3 diluents with aqueous soybean extract on liquid semen quality in Ongole crossbred bull

et al. 2023

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Background and Aim: Egg yolk (EY) is commonly used as an extracellular cryoprotectant in semen diluents but has some negative effects. Therefore, this study aimed to assess the potential of lecithin derived from plants, such as soybeans, as an alternative extracellular cryoprotectant and to characterize liquid semen quality of Ongole crossbred bulls using a modified caudal epididymis plasma-3 [CEP-3 (m)] as a base diluent and aqueous soybean extract (ASE). Materials and Methods: A bull with progressive motility (PM) of fresh semen >70% was used. Two soybean extracts were also used, namely, ASE 1 and ASE 2, obtained by extraction procedures 1 and 2, respectively. The study was conducted using an experimental design with 11 treatments and ten replications, with diluents comprising different levels of ASE 1 and ASE 2, as well as a positive control with 10% EY. The parameters measured were motility (M) and its kinetic parameters, including PM, M, velocity curve linear, velocity straight linear, velocity average pathway, linearity, straightness, wobble, amplitude lateral head beat cross frequency, and hyperactivity using computer-assisted sperm analysis, viability, and spermatozoa abnormalities. Results: The CEP-3(m) diluent formula and ASE 1 at a 30% level maintained the PM of spermatozoa up to day 5 (40.7% ± 16.1%) of cold storage. Meanwhile, the CEP-3(m) diluent formula and ASE 2 could only maintain PM >40% until day 3 (42.1% ± 13.5%) of cold storage at a 30% level. The CEP-3(m) diluent and ASE 1 at a level of 25%–30% supported spermatozoa life (viability) up to day 5 with a value >80% (81.8 ± 3.5; 86.4 ± 2.6). The abnormality value of spermatozoa in various diluents during cold storage on days 0–5 was below 20%. Conclusion: Soybean extracts 1 and 2 can substitute EYs as extracellular cryoprotectants in modified CEP-3 basic diluents. Soybean extract 1 can support the life of spermatozoa up to day 5 but may cause the viscosity and movement of spermatozoa to be hyperactive. Soybean extract 2 can support the life of spermatozoa up to the 3rd day of cold storage and produces progressive (non-rotating) movement patterns. Further, research is recommended with higher levels of extract 2. Keywords: aqueous soybean extract, liquid semen, Ongole crossbred cattle.

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Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

Cited by 6 publications

References 22 publications

The Supplementation of Bee Bread Methanolic Extract to Egg Yolk or Soybean Lecithin Extenders Can Improve the Quality of Cryopreserved Ram Semen

The Supplementation of Bee Bread Methanolic Extract to Egg Yolk or Soybean Lecithin Extenders Can Improve the Quality of Cryopreserved Ram Semen

Study Motility of Ongole Crossbred Semen with Soybean-Based Diluents Stored at Ambient Temperature (24-27 0C)

Effect of modified CEP-3 diluents with aqueous soybean extract on liquid semen quality in Ongole crossbred bull

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