Effectiveness of multiple pre-ischemia electro-acupuncture on attenuating lipid peroxidation induced by cerebral ischemia in adult rats

Siu, Flora K.W.; Lo, Samuel Chun‐Lap; Leung, Mason C.P.

doi:10.1016/j.lfs.2004.02.025

Cited by 30 publications

(21 citation statements)

References 23 publications

(23 reference statements)

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“…Despite the fact that peroxidative effects of EA were demonstrated here, in contrast with other studies 6 , 10 Hz electro stimulation was not used by other researchers. Published studies used low-frequency EA (2 Hz), in multiple sessions.…”

Section: Discussioncontrasting

confidence: 57%

“…Siu et al 6 electro-stimulated the acupoints GB-20, located on the posterior aspect of the neck, below the occipital bone, in the depression between the sternocleidomastoid muscle and trapezius muscle and the ST-36, using multiple applications before cerebral ischemia and concluded that 2Hz EA could partly regulate the lipid peroxidation in cerebral ischemia. GB-20 and ST-36 had a similar beneficial effectiveness 6 . In our study, the use of EA induced a significant raise in MDA concentration in the liver (Figure 3) and the kidney (Figure 4) in EA10Hz and EA100Hz groups.…”

Section: Discussionmentioning

confidence: 99%

“…Electroacupuncture (EA) is a modification of this technique where small electrical currents are applied to needles previously inserted in the body and appears to have more consistently reproducible results in many specific clinical and research settings [2][3][4] . EA applied to Zusanli (ST-36) acupoint reduced lipid peroxidation in experimental models of ischemia/reperfusion such as rat spinal cord 5 , brain 6 and blood serum 7 and pig heart muscle 8 . Chakrabarti et al 9 investigated the effect of single, acute (7 pulses/sec., 0.75 volt) and chronic (4 pulses/sec., 0.75 volt) electroacupuncture treatment on alternate days for a period of 21 days on hepatic functions of rats.…”

Section: Introductionmentioning

confidence: 99%

“…The integrity of the cells as well as the entire antioxidant system rely on the adequate supply of NADPH 11 . Many papers have demonstrated the effects of MA and EA on ST-36 acupoint in attenuating oxidative stress in different experimental diseases [5][6][7][8][9] . Furthermore, the use of ketamine+xylazine, an anesthetic mixture often used in experimental studies may induce some degree of oxidative stress in a health animal [12][13] .…”

Section: Introductionmentioning

confidence: 99%

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Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Silva¹,

Figueiredo²,

Dias³

et al. 2011

Acta Cir. Bras.

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PURPOSE:Investigate the effects of a single electroacupuncture (EA) session at acupoints Zusanli (ST-36) and Zhongwan (CV-12) combined in regulating oxidative stress in liver and kidney in anesthetized rats. METHODS: Eighteen healthy rats randomly assigned to 3 groups (n=6) were anesthetized intraperitoneally with ketamine (90mg kg -1 body weight) + xylazine (10mg/kg body weight): G-1: Control (anesthesia), G-2: anesthesia+EA10Hz and 10 mA, 10 Hz) applied to right ST-36 and CV-12 acupoints for 30 minutes. G-3 was likewise treated, using a tenfold higher frequency (100 Hz). G6PDH activity, malondialdehyde (MDA) and glutathione (GSH) levels were assayed spectrophotometrically. RESULTS: Liver MDA and GSH concentrations increased significantly in rats submitted to EA 10Hz (p<0.01) and EA 100Hz (p<0.001), compared with control G-1. Liver and kidney G6GPH activity decreased significantly in G-2 (p<0.01) and G-3 (p<0.001) compared with G-1 in EA100Hz rats. A similar pattern was found in kidney G6PDH activity in EA10Hz rats. CONCLUSION: Single 30-minute EA 10/100Hz session enhances lipid peroxidation and simultaneously reduces oxidative stress in liver and kidney tissues in a rat model. Keywords: Acupuncture. Electroacupuncture. Lipid Peroxidation. Oxidative Stress. Rats. RESUMO OBJETIVO: Investigar os efeitos de uma única sessão de eletroacupuntura (EA) aplicada nos acupontos Zusanli (E-36) e Zhongwan (RM-12) simultaneamente, na regulação do estresse oxidativo no fígado e rins em ratos anestesiados. MÉTODOS: Dezoito ratos sadios, distribuídos aleatoriamente em três grupos (n = 6), foram anestesiados com cetamina (90mg/kg de peso) + xilazina (10mg/kg de peso): G-1: Controle (anestesia), G-2: anestesia + EA10Hz e G-3: anestesia + EA100Hz. Os ratos do grupo G-2 foram submetidos à EA (ondas quadradas pulsadas, 10 mA, 10 Hz) aplicada aos acupontos ST-36 direito e VC-12 por 30 minutos. Nos ratos do grupo G-3 utilizou-se uma freqüência dez vezes maior (100 Hz). A atividade da enzima G6PDH e as concentrações de malondialdeído (MDA) e glutationa (GSH) foram verificadas por espectrofotometria. RESULTADOS: As concentrações hepáticas de MDA e GSH aumentaram significativamente nos ratos submetidos à EA, utilizando 10Hz (p <0,01) e 100Hz (p <0,001), comparado com o controle. A atividade de G6GPH diminuiu significativamente no G-2 (p <0,01) e G-3 (p <0,001) no fígado e no rim em comparação ao grupo G-1 em ratos tratados com 100Hz. CONCLUSÃO: Uma única sessão de EA 10/100Hz por 30 minutos aumenta a peroxidação lipídica e simultaneamente reduz o estresse oxidativo no fígado e no rim de ratos sadios. Descritores: Acupuntura. Eletroacupuntura. Peroxidação de Lipídeos. Estresse Oxidativo. Ratos. Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats

Silva¹,

Figueiredo²,

Dias³

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…ST36 is set 5 mm below the head of the fibula under the knee joint and 2 mm lateral to the anterior tubercle of the tibia [23]. As one of the widely used points in traditional Chinese medicine (TCM), the neuroprotective effects of ST36 in both animal and human studies are well established [24][25][26][27]. The control point is located approximately 10 mm above iliac crest (Fig.…”

Section: Acupuncture Treatmentmentioning

confidence: 99%