Effect of Vitamin E and Polyunsaturated Fatty Acids on Cryopreserved Sperm Quality inBos taurusBulls Under Testicular Heat Stress

Abstract

Taurine bulls are highly susceptible to heat stress, leading to increased oxidative stress (OS) and impaired sperm viability. Polyunsaturated fatty acids (PUFAs) supplementation can be an alternative to improve semen quality, which also results in more sperm susceptibility to lipid peroxidation. Moreover, this deleterious effect can be exacerbated in animals affected by heat stress. Vitamin E is a key antioxidant that counteracts lipid peroxidation of sperm membrane caused by OS. Thus, combining PUFAs with vitamin E may improve sperm quality. In this context, this study aimed to evaluate the effect of interaction between PUFAs and vitamin E on sperm quality in Bos taurus bulls under testicular heat stress. Sixteen taurine bulls under testicular heat stress were randomly assigned in four groups: Control, Vitamin E, PUFA, and PUFA + Vitamin E. All groups lasted for 60 days. Samples were cryopreserved/thawed and analyzed for motility variables (CASA), membrane and acrosome integrity, mitochondrial activity, susceptibility to oxidative stress, DNA integrity, and sperm-binding capacity. Results showed that vitamin E had a beneficial effect on some sperm characteristics, whereas PUFA supplementation had an adverse effect when the two treatments were evaluated separately. Finally, the association between PUFAs and vitamin E did not improve sperm quality.

Effect of Dietary Vitamin E and Selenium Supplementation on Physiological Responses and Reproductive Performance in Holstein Friesian Bulls during Humid Hot Summer

“…The several previous studies in farm animals showed positive effects of Se and/or Vit. E on semen quality in goats (Shi et al, 2010), sheep (Liu et al, 2014) and bulls (Losano et al, 2018). Contrary to current study, no effect of Se and/or Vit.…”

“…Vit. E has also been reported to reduced testicular oxidative stress (Tvrdá et al, 2016) and improve sperm quality in bulls under testicular heat stress (Losano et al, 2018). Vit.…”

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

“…It well known that estimation of melandialdehyde (MDA) in the seminal plasma is an end product of lipid peroxidation helps to assessing the extent of cellular damage; when cells membrane damage caused irreversible lose in essential metabolites and enzymes (Talluri et al, 2017). Also, Losano et al (2018) demonstrated that extender contains high PUFAs resulting in more susceptible spermatozoa membrane to ROS that leads to damages in membrane matrix and causes destruction of membrane structural and biochemical organs of sperm. High enzyme activity around spermatozoa in the extra medium is sign to injury sperm membrane regard cold shock as well as unsuitable concentration of extender ingredients (Sharafi et al, 2015;Wang et al, 2016).…”

Selenium and vitamin E supplementation enhances the antioxidant status of spermatozoa and improves semen quality in male dogs with lowered fertility

“…Vitamin E plays a key role in protecting spermatozoa against lipid peroxidation (Agarwal, ; Zubair, ). Supplementation with vitamin E showed a beneficial effect on the semen quality in humans (Suleiman, Ali, Zaiu, El‐Malik, & Nasr, ), boars (Marin‐Guzman et al., , ), bulls (Losano et al., ) and male sheep (Yue, Yan, Luo, Xu, & Jin, ).…”