Selenium and vitamin E supplementation enhances the antioxidant status of spermatozoa and improves semen quality in male dogs with lowered fertility

Abstract

Studies showed a beneficial effect of supplementation with selenium (Se) and vitamin E on semen quality. The aim of the study was to investigate the effect of Se and vitamin E supplementation on the antioxidant status of spermatozoa and semen quality in dogs with lowered fertility. Ten dogs were supplemented daily with Se (6 μg/kg organic Se yeast) and vitamin E (5 mg/kg) per os for 60 days. Control group consisted of 10 males without the supplementation. Semen was collected on day 0, 30, 60 and 90. Sperm quality parameters were evaluated using CASA and a microscope. Concentrations of Se and vitamin E in blood as well as glutathione peroxidase (GSH-Px) activity and total antioxidant capacity (TAC) in the spermatozoa were determined. After 60 days of supplementation the concentration of spermatozoa, the majority of motility indicators and the percentage of normal morphology and live spermatozoa increased significantly (p < .05). An increase (p < .05) in concentration of Se and vitamin E in blood and GSH-Px-activity and TAC in the spermatozoa was detected. The study results indicate that Se and vitamin E supplementation for 60 days enhances the antioxidant status of spermatozoa and improves the quality of the semen in dogs with lowered fertility.

Effect of Dietary Vitamin E and Selenium Supplementation on Physiological Responses and Reproductive Performance in Holstein Friesian Bulls during Humid Hot Summer

“…The positive response of Vit. E and Se on sperm motility in our study could be explained due to their antioxidant properties of these nutrients (Domosławska et al, 2018) thereby decreasing oxidative stress during summer (Chauhan et al, 2014). The several previous studies in farm animals showed positive effects of Se and/or Vit.…”

Canine babesiosis – a disease rarely considered in the context of male infertility

“…Supplementation with antioxidants can lead to sperm improvement, as was demonstrated in a previous report [ 23 ]. In the cases described, supplementation with selenium and Vitamin E as antioxidants also improved the quality of semen and restored fertility in two dogs.…”

Effect of selenium and pentoxifylline on expression of CATSPER1 and 2 genes and FSH/LH levels in treated mice by dexamethasone

“…Therefore, it seems that CATSPER1 gene expression is more sensitive to Se than CATSPER2. In another study, Se increased semen antioxidant status and sperm parameters in dogs with low sperm quality (Domosławska, Zdunczyk, Franczyk, Kankofer, & Janowski, ). However, as yet there have been no reports regarding the effect of pentoxifylline on CATSPER gene expression.…”