Effect of Triticum monococcum glutenin loci on cookie making quality and on predictive tests for bread making quality

Abstract

The effects of Triticum monococcum glutenin loci on cookie making quality and predictive tests for bread making quality were evaluated in recombinant substitution lines (RSLs) between chromosome 1A m from T. monococcum and chromosome 1A from Chinese Spring. All four combinations of high molecular weight (HM r -GS) and low molecular weight glutenin alleles (LM r -GS) were studied in a factorial design to evaluate their interactions. Grain protein content was used as a covariable to evaluate the effect of these loci independently of the variation in protein content among lines. No significant interactions were detected indicating an additive effect. RSLs carrying the HM r -GS from T. monococcum showed a 13·6% increase in SDS sedimentation volume (p=0·004) and a significant reduction in cookie diameter (−5·2%, p=0·02), and cookie quality (−6·8%, p=0·02). RSLs carrying the LM r -GS from T. monococcum showed a significant decrease in the proportion of polymeric protein (−2·8%, p<0·0001), SDS sedimentation volume (−8·1%, p=0·03) and gluten strength (−16·5%, p=0·01), and a significant increase in cookie quality (5·9%, p=0·05). The T. monococcum LM r -GS allele has potential value to be used in soft wheat breeding programs. These results suggest that diploid T. monococcum could be a valuable source for new allelic variation for storage proteins loci and new quality characteristics.

Genetic Diversity for Wheat Improvement as a Conduit to Food Security

“…The genes coding LMW-GS have been studied in the genera Elytrigia (Gupta and Shephard, 1990), Elymus (Obukhova et al, 1997), Dasypyrum (Blanco et al, 1991), and Hordeum (Atienza et al, 2002). Similarly, the other species that have been analyzed for LMW-GS include T. monococcum, T. urartu (Lee et al, 1999;Tranquilli et al, 2002), T. turgidum var. tauschii (DD) has been an important source for genetic studies of LMW-GS (Gianibelli et al, 2000;Hsam et al, 2001;Pfluger et al, 2001;Vensel et al, 1997;Zheng et al, 2006) and exhibited greater variation in the coding sequence of LMW-GS (Lafiandra et al, 2000;Masci et al, 1991).…”

Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum

“…T. monococcum ssp. monococcum was considered as a valuable resource for wheat bread-making quality improvement (Tranquilli et al 2002). This species has abundant allele variations of HMW-GSs (Waines and Payne 1987;Ciaffi et al 1998;Ma et al 2007;Hu et al 2012).…”

Molecular genetic analysis of phosphomannomutase genes in Triticum monococcum

“…Some disease resistance genes in T. monococcum were transferred to common wheat through marker-assisted selection [7,8]. Puroiudoline and high-molecular-weight glutenin subunit genes in T. monococcum were also introgressed into common wheat to improve grain milling and processing qualities [11][12][13][14].…”