Molecular genetic analysis of phosphomannomutase genes in Triticum monococcum

Abstract

In higher plants, phosphomannomutase (PMM) is essential for synthesizing the antioxidant ascorbic acid through the Smirnoff-Wheeler pathway. Previously, we characterized six PMM genes (TaPMM-A1, A2, B1, B2, D1 and D2) in common wheat (Triticum aestivum, AABBDD).Here, we report a molecular genetic analysis of PMM genes in Triticum monococcum (A m A m ), a diploid wheat species whose A m genome is closely related to the A genome of common wheat. Two distinct PMM genes, TmPMM-1 and TmPMM-2, were found in T. monococcum. The coding region of TmPMM-1 was intact and highly conserved. In contrast, two main TmPMM-2 alleles were identified, with TmPMM-2a possessing an intact coding sequence and TmPMM-2b being a pseudogene. The transcript level of TmPMM-2a was much higher than that of TmPMM-2b, and a bacterially expressed TmPMM-2a recombinant protein displayed relatively high PMM activity. In general, the total transcript level of PMM was substantially higher in accessions carrying TmPMM-1 and TmPMM-2a than those harboring TmPMM-1 and TmPMM-2b. However, total PMM protein and activity levels did not differ drastically between the two genotypes. This work provides new information on PMM genes in T. monococcum and expands our understanding on Triticeae PMM genes, which may aid further functional and applied studies of PMM in crop plants.

Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen tolerance

“…A PCR mix (0.02 cm 3 ) was done by the 2× TB Green Premix Ex Taq II (TaKaRa, Dalian, China) user manual. Thermoamplification was performed in a 96-well plate (Applied Biosystems, Carlsbad, USA) following our previous procedure (Yu et al 2015). Using actin as an internal control, expression of a target gene was compared to an internal gene (Chen et al 2018).…”

Enhanced amount and quality of alginate-like exopolysaccharides in aerobic granular sludge for the treatment of salty wastewater

“…The role of PMM is to catalyze the interconversion between mannose-6-phosphate (M6P) and mannose-1-phosphate (M1P), which are both key precursors of alginate synthesizing (Fig. 5) (May et al 1991;Yu et al 2015). Zielinski et al (1992) has reported that the maximum activation to algC expression was achieved at 0.3 M NaCl, and a higher concentration of NaCl would result in decreased activation.…”