Effect of the size and surface charge of polymer microspheres on their phagocytosis by macrophage

Tabata, Yasuhiko; Ikada, Yoshito

doi:10.1016/0142-9612(88)90033-6

Cited by 485 publications

(312 citation statements)

References 17 publications

Supporting

Mentioning

289

Contrasting

Unclassified

Order By: Relevance

“…1A to be of a size range (1-10 μm) recognizable by antigen presenting cells [16,29]. About 0.8 μg of peptide was coupled to per mg of bulk microspheres which contain only 3 μg polylysine (Fig.…”

Section: C-tt2-ctp35 Antigen Conjugated To Plga Microsphere Surfacementioning

confidence: 99%

“…As microparticles of PLGA polyesters degrade to produce lactic and glycolic acids in vivo, encapsulated immunogens are slowly and continuously released resulting in repeated stimulation of lymphocytes, including antigen presenting cells, eliminating the need for multiple immunizations. In addition to antibody production, PLGA microparticles have been observed to induce phagocytosis and elicit cellular immune responses in contrast to aluminum-based adjuvants [15][16][17]. Despite this progress, until now PLGA microspheres encapsulating hCG peptide antigens have been unsuccessful to achieve strong immune responses when administered in safe (non-oily) aqueous vehicles.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Injectable polymer microspheres enhance immunogenicity of a contraceptive peptide vaccine

Cui

Stevens

Schwendeman

2007

Vaccine

View full text Add to dashboard Cite

Advanced contraceptive peptide vaccines suffer from the unavailability of adjuvants capable of enhancing the antibody response with acceptable safety. We sought to overcome this limitation by employing two novel poly(lactic-co-glycolic acid) (PLGA) microsphere formulations to deliver a synthetic human chorionic gonadotropin (hCG) peptide antigen co-synthesized with a T-cell epitope from tetanus toxoid, C-TT2-CTP35: surface-conjugated immunogen to induce phagocytosis; and encapsulated peptide to provide a depot effect, with MgCO 3 co-encapsulated in the polymer to neutralize acidity from the biodegrading PLGA polyester. A single immunization of encapsulated peptide in rabbits elicited a stronger antibody response with equivalent duration relative to a positive control -three injections of the peptide administered in a squalene-based water-in-oil emulsion. Surface-conjugated peptide was less effective but enhanced antibody levels at 1/5 the dose, relative to soluble antigen. Most remarkable and unexpected was the finding that co-encapsulation of base was essential to attain the powerful adjuvant effect of the PLGAMgCO 3 system, as the MgCO 3 -free microspheres were completely ineffective. A promising contraceptive hCG peptide vaccine with acceptable side effects (i.e., local tissue reactions) was achieved by minimizing PLGA and MgCO 3 doses, without significantly affecting antibody response.

show abstract

Section: C-tt2-ctp35 Antigen Conjugated To Plga Microsphere Surfacementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Injectable polymer microspheres enhance immunogenicity of a contraceptive peptide vaccine

Cui

Stevens

Schwendeman

2007

Vaccine

View full text Add to dashboard Cite

show abstract

“…Similarly, D(v,0.1), D(v,0.5) and D(v,0.9) represent the 10%, 50% and 90% size intervals of the volume distribution of particles. D [4,3] represents the equivalent volume mean, also known as the De Brouker mean [8].…”

Section: Resultsmentioning

confidence: 99%

“…Tipper et al [6] reported a wide variation in the 115.59 114.14 D [4,3] 63.75 62.81 Particle concentration (vol. %) 0.0287 0.0233 mass, and by inference volume, distributions of ex vivo wear debris.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies published to date have examined the in vitro interaction of debris-like particles with macrophages. These studies have shown a number of particle parameters including size [1], material [2], morphology [3] and surface charge [4] to be capable of eliciting an adverse cellular response. It is therefore important that investigations to quantify the in vivo and in vitro response to wear debris should be underpinned by accurate and comprehensive debris particle characterization.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Elfick

Green

Pinder

et al. 2000

Journal of Materials Science: Materials in Medicine

View full text Add to dashboard Cite

Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. The accurate and detailed characterization of arti®cial joint wear debris is important in determining both the wear rate of prostheses and understanding the role that the debris plays in the development and progression of aseptic loosening. The novel application of low angle laser light scattering (LALLS) to the particle size characterization of ultra high molecular weight polyethylene (UHMWPE) wear debris is described. The results demonstrate that both ex vivo and in vitro origin wear debris samples, at concentrations typical of those produced via an alkali-digestion retrieval route, can be reproducibly analyzed via LALLS. Because the LALLS route enables particle size analysis of the entire debris sample to be acquired non-destructively and whilst in suspension, artefacts associated with ®ltering, drying and agglomeration of debris are avoided, in contrast to currently used techniques such as ®ltration and scanning electron microscopy (SEM) observation.

show abstract

Different effects of phagocytosable particles during bone formation versus remodeling

Goodman

Aspenberg

Song

et al. 1996

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Previously, small phagocytosable particles of high density polyethylene (HDPE) but not Ti6-Al4-V alloy, at a concentration of 10(8) particles/mL inhibited net bone formation in vivo after 3 weeks in the bone harvest chamber (BHC). These findings reflected the effects of particles during the phase of bone ingrowth. In this study, we tested whether these effects persisted or were different during the phase of bone maturation and remodeling. BHCs were bilaterally implanted in mature male NZW rabbits. After a 6-week period for osseointegration, the contents of the chamber were harvested and discarded. One percent sodium hyaluronate, the carrier, was then placed within the canal of the chambers bilaterally and the tissue within the chambers was harvested 3 weeks later. HDPE particles were then inserted unilaterally for a 3-week period, followed by Ti6-Al4-V for 3 weeks, HDPE for 6 weeks, and Ti6-Al4-V for 6 weeks. The side chosen for each treatment was switched consecutively; the nonimplanted, contralateral chamber served as a control. At 3 weeks the control treatments yielded trabeculae of woven bone in a fibrovascular stroma. By 6 weeks, the peripheral trabeculae were thicker, and a central marrow cavity was developing. Bone ingrowth was less with HDPE particles at 3 and 6 weeks compared to controls. Ti6-Al4-V particles did not inhibit bone ingrowth at 3 weeks but showed a trend at 6 weeks. The characteristics of particles affect the differentiation, maturation, and remodeling of mesenchymal tissue differently.

show abstract

Effect of the size and surface charge of polymer microspheres on their phagocytosis by macrophage

Cited by 485 publications

References 17 publications

Injectable polymer microspheres enhance immunogenicity of a contraceptive peptide vaccine

Injectable polymer microspheres enhance immunogenicity of a contraceptive peptide vaccine

Untitled

Different effects of phagocytosable particles during bone formation versus remodeling

Contact Info

Product

Resources

About