Effect of Temperature and Gamma Radiation on Salmonella Hadar Biofilm Production on Different Food Contact Surfaces

Abstract

Salmonella is a pathogen transmitted by foods and it is one of the most important target bacteria in food irradiation studies. Few works were carried out on the effectiveness of gamma radiation against biofilms formed by this bacterium. Salmonella can form a biofilm on different material surfaces. The physicochemical properties of surfaces and environmental factors influence the adhesion of this pathogen. The present study investigated the effect of gamma radiation (1 and 2 kGy) and temperature (28°C and 37°C) on the development of Salmonella Hadar biofilm on polyvinyl chloride (PVC), glass, cellophane paper (CELLO), and polystyrene (POLY). The obtained results indicated that biofilm production is surface and temperature dependent. In addition, biofilm formation decreased significantly after gamma irradiation at either 1 or 2 kGy doses. However, the agfD and adrA genes expression did not demonstrate significant decrease. This work highlighted that gamma radiation treatment could reduce the biofilm formation of Salmonella enterica serovar Hadar on different food contact surfaces.

Biofilm Forming Ability of Broiler Chicken Meat Associated <em>Salmonella</em> spp. on Food Contact Surfaces

“…The capability of Salmonella spp. in biofilm formation on plastic, metal, glass, or rubber surfaces were reported in many previous studies (Somers et al, 1994;Dhir and Dodd, 1995;Jones and Bradshaw, 1996;Humphrey et al, 1997;Hood and Zottola, 1997;Wong, 1998;Sommer et al, 1999;Chae and Schraft, 2000;Leriche and Carpentier, 2000;Sinde and Carballo, 2000;Joseph et al, 2001;Ben Miloud Yahia et al, 2018). Similarly, all isolated Salmonella spp.…”

“…Biofilm formation in food processing environments has great importance as it can act as a chronic source of microbial contamination which may lead to food spoilage, and disease transmission (Stepanovic et al, 2004;Souza et al, 2014). There are many factors which affect the formation of biofilm, and many researchers have reported that the nutritional condition in the medium is an important factor for the formation of biofilms (Dewanti et al, 1995;Djordjevic et al, 2002;Stepanovic et al, 2003a, Stepanovic et al,2003bBen Miloud Yahia et al, 2018).…”

Synergistic Antitumor Effect of Combined Radiotherapy and Engineered Salmonella typhimurium in an Intracranial Sarcoma Mouse Model

“…More survival benefits were seen in the adjuvant group, perhaps because the tumor cells were more susceptible to SLpFlaB infection after RT [ 32 , 33 ]. In the neoadjuvant group, the lesser survival benefits observed might be due to impaired biological functions of SLpFlaB after RT, affecting any immune effect [ 34 ]. The exact mechanisms require further research.…”