The combined effect of stressful factors (temperature and pH) on the expression of biofilm, stress, and virulence genes in Salmonella enterica ser. Enteritidis and Typhimurium

Badie, Fereshteh; Saffari, Mahmood; Moniri, Rezvan; Alani, Behrang; Atoof, Fatemeh; Kazemi, Ahmad

doi:10.1007/s00203-021-02435-y

Cited by 7 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As vaccine delivery strains, they have vast dynamics and alterations in gene expression to sustain oxidative and acid stress in the host system. Such stresses minimize the survivability of the attenuated strains (Figure 2 D), but a stressful acidic environment has exaggerated the expression of biofilm, stress, and virulence genes [ 33 ]. Modulation of virulence genes in attenuated strains (Figure 2 E) indicates their improved adhesion and strengthens the delivery strain phenotype.…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Aganja

Sivasankar

Hewawaduge

et al. 2022

Vet Res

View full text Add to dashboard Cite

In the present study, two prospective Salmonella delivery strains, JOL2782 and JOL2837, were developed by gene deletions of lon and cpxR, which are related to cellular adhesion and intracellular survival. Additionally, sifA deletion was introduced for JOL2782, which confers immune susceptibility and improves antigen delivery. Similarly, the rfaL deletion and lpxE substitution for pagL were accomplished in JOL2837 to reduce virulence and endotoxicity. Thus, enhanced adhesion and invasion and reduced intracellular survival were attained. Furthermore, aspartic acid auxotrophic (asd) was deleted to impose Darwinian selection on retention of the foreign antigen-expressing plasmid. Both delivery strains induced sufficient cytokine expression, but the level was significantly lower than that of the wild-type strain; the lowest cytokine expression was induced by the JOL2837 strain, indicating reduced endotoxicity. In parallel, IgG production was significantly enhanced by both delivery strains. Thus, the innate and adaptive immunogenicity of the strains was ensured. The environmental safety of these strains was ascertained through faecal dissemination assays. The nonpathogenicity of these strains to the host was confirmed by body weight monitoring, survival assays, and morphological and histological assessments of the vital organs. The in vitro assay in murine and human cell lines and in vivo safety assessments in mice suggest that these novel strains possess safety, invasiveness, and immunogenicity, making them ideal delivery strains. Overall, the results clearly showed that strain JOL2782 with sifA deletion had higher invasiveness, demonstrating superior vaccine deliverability, while JOL2837 with lpxE substitution for pagL and rfaL deletion had outstanding safety potential with drastically abridged endotoxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Aganja

Sivasankar

Hewawaduge

et al. 2022

Vet Res

View full text Add to dashboard Cite

show abstract

“…Additionally, the presence/absence of genes (and its frequency) associated with stress tolerance, virulence, and antibiotic resistance in Salmonella could help in developing a differential virulence profile that could aid in re-evaluating the existing infectivity and outbreak predictive estimates for Salmonella enterica . For example, the genes associated with biofilm production ( adrA , bapA ), virulence ( hilA , invA , invC, invG , prgH ), and temperature stress ( rpoS , rpoE , and rpoH ) have been previously associated with exposure of different serovars of Salmonella such as Enteritidis and Tyhpimurium to stressful temperature and pH conditions ( Sirsat et al, 2015 ; Badie et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Machine learning to predict foodborne salmonellosis outbreaks based on genome characteristics and meteorological trends

Karanth

Patel

Shirmohammadi

et al. 2023

Current Research in Food Science

View full text Add to dashboard Cite

Can plastic pollution drive the emergence and dissemination of novel zoonotic diseases?

Ormsby,

Woodford,

Quilliam

2024

Environmental Research

View full text Add to dashboard Cite

The combined effect of stressful factors (temperature and pH) on the expression of biofilm, stress, and virulence genes in Salmonella enterica ser. Enteritidis and Typhimurium

Cited by 7 publications

References 40 publications

Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Machine learning to predict foodborne salmonellosis outbreaks based on genome characteristics and meteorological trends

Can plastic pollution drive the emergence and dissemination of novel zoonotic diseases?

Contact Info

Product

Resources

About