Effect of sulphachloropyrazine on antioxidative systems in blood and liver of broilers

Abstract

This report describes the effects of therapeutic doses of coccidiocid sulphachloropyrazine on enzymatic and non-enzymatic antioxidative systems in haemolysed blood and liver homogenate from broilers (glutathione, glutathione-reductase, glutathione-peroxidase, peroxidase, superoxidedismutase, xantine-oxidase and lipid peroxidation). The in vivo investigation was carried out on 120 heavy-line broilers (Arbor acres) of both sexes. One-day-old broilers were randomly distributed into 2 groups, each numbering 60 individuals of both sexes: Group 1 -control group; Group 2 -group of broilers inoculated with laboratory derived coccidia species on the 21 st day-ofage. When symptoms of coccidiosis appeared (30 th day-of-age), blood sampling and decapitation of 20 chickens were carried out (Group 2a). The remaining broilers were treated with therapeutic doses of sulphachloropyrazine (60 ppm). Decapitation of 20 chickens was carried out after the therapy was concluded (38 th day-of-age -Group 2b). Infection of broilers with coccidia intensified free radical processing in haemolysed blood and liver homogenate. This was evident from the increased levels of lipid peroxidation and the catalytic activity of almost all examined enzymes (SOD, GSHPx and Px). Therapeutic doses of sulphachloropyrazine inhibited free-radical activity induced by disease and establishing of physiological values of lipid peroxidation and catalase activity of examined enzymes.

Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers

“…This seemed reasonable given that zinc bacitracin and fermented feed possessed the antimicrobial properties, which are possible to control the invading pathogenic microorganisms [10]. It has generally been known that infections may be linked to the increased metabolic rate in animals due to the increased need for maintenance (recovery) energy [23]. In this study, the hemoglobin, erythrocytes, and hematocrit values were lower (p<0.05) in BACI and FERM than that in CONT and FERB chickens.…”

Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates

“…Unlike those of mammals, presence of intact but transcriptionally inactive nucleus and other cell organelles are the most persuasive nature of mature chicken erythrocyte 34, 35 . Hence they are considered as animal cell model to monitor the response to the drug 36, 37 .…”

“…Chicken erythrocyte were used as a model to scrutinize the size depended intake and effect of ZnONPs and ZnONPs conjugate with FA, on structural damage, hemolytic potential, cell viability, ROS generation, and DNA damage. Unlike those of mammals, presence of intact but transcriptionally inactive nucleus and other cell organelles are the most persuasive nature of mature chicken erythrocyte 34 , 35 . Hence they are considered as animal cell model to monitor the response to the drug 36 , 37 .…”

Effect of synbiotic on growth and antioxidant status of blood in broiler chicken

“…Level of hemoglobin, necessary for the expression of the enzymatic activities in hemolysed blood, was determined using a commercial test ('Dialab', Vienna, Austria) on a spectrophotometer (Multiscan MCC 340, Finland). The SOD (EC 1.15.1.1) activity was determined by the spectrophotometric method based on the inhibition of adrenaline reduction to adrenochrome at pH 10.2 (Kostadinović et al, 2011). The GPx (EC 1.11.1.9) activity was determined by spectrophotometric measurement of absorbance at 412 nm with cumenhydroperoxide as the substrate (Chiu and Stults, 1976).…”

“…Malondialdehyde concentration (MDA) in serum was measured by the 2-thiobarbituric acid assay (Draper et al, 1993). The glutathione (GSH) content in the blood hemolysate was determined using the method reported by Kostadinović et al (2011).…”