Effect of probiotic on reproductive performance in female livebearing ornamental fish

Abstract

A probiotic bacterial strain, Bacillus subtilis, isolated from the intestine of Cirrhinus mrigala (Hamilton), was incorporated in ¢sh feed at four di¡erent concentrations (5 Â 10 8 cells g À 2 , 5 Â 10 7 cells g À 2 , 5 Â 10 6 cells g À1 and 5 Â 10 5 cells g À1 ) and fed to four species of livebearing ornamental ¢sh, Poecilia reticulata (Peters), Poecilia sphenops (Valenciennes), Xiphophorus helleri (Heckel) and Xiphophorus maculatus (Gunther) for one year duration to observe the effect of dietary probiotic supplementation on their reproductive performance. Sixty virgin females of each species were stocked separately in circular FRP tanks (350 L) and fed diets with varying levels of probiotic cells and control feed. Broodstock performance was evaluated based on gonadosomatic index (GSI), fecundity and fry production of female broodstock. The results showed that supplementation of feed with probiotics increased signi¢cantly (Po0.05) the GSI, fecundity and fry production of spawning females and length and weight of fry in all the four species of ¢sh. The number of dead and deformed fry were also signi¢cantly lower (Po0.05) in ¢sh fed with the probiotic feeds. The use of higher concentration of the probiont in diet did not always lead to sig-ni¢cantly improved reproductive performance of the spawners. Collectively, this study showed that female livebearers bene¢t from inclusion of probiotics in diet during their reproductive stages.

Evaluation of probiotic strain Bacillus subtilis C-3102 as a feed supplement for koi carp (Cyprinus carpio)

“…With the development of the ornamental fish trade, more investigations of the effects of probiotics on ornamental fish have been conducted [19][20][21][22]. Spores of Bacillus strains are thermo-stabile which makes them easy to incorporate into compound diets.…”

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

“…Penggunaan bakteri probiotik sudah sangat meluas di hampir semua kegiatan budidaya diantaranya, pemeliharaan larva kepiting (Nogami & Maeda, 1992), larva rajungan (Susanto et al, 2005), dan budidaya artemia (Villamil et al, 2003). Pada budidaya ikan, penggunaan probiotik dijumpai pada pemeliharaan rainbow trout (Brunt & Austin, 2005;Brunt et al, 2007), ikan karper (Labeo rohita) (Kumar et al, 2006;Banerjee et al, 2007), pematangan gonad ikan hias (Ghosh et al, 2007), pemeliharaan ikan nila (Tilapia nilotica) (Aly et al, 2008a, Wang et al, 2008, larva ikan sebelah (flatfish) Senegalese sole (Makridis et al, 2008), larva gilthead sea bream (Sparus aurata, L) (Suzer et al, 2008), dan budidaya ikan kakap putih (seabass) (Rengpipat et al 2008). Macey & Coyne (2005), melaporkan penggunaan bakteri probiotik pada budidaya abalon.…”

TEKNIK APLIKASI BAKTERI PROBIOTIK PADA PEMELIHARAAN UDANG WINDU (Penaeus monodon) DI LABORATORIUM

“…Selain digunakan pada budidaya udang vannamei, udang windu, dan udang galah (Meunpol et al, 2003;Vaseeharan & Ramasamy, 2003;Vaseeharan et al, 2004;Lio-Po et al, 2005;Gunarto et al, 2006aGunarto et al, ,2006bLi et al 2006;Nejad et al, 2006;Vijayan et al, 2006Keysami et al, 2007Castex et al, 2008;Decamp et al, 2008), penggunaan probiotik juga sudah mulai berkembang pada beberapa komoditas budidaya lainnya seperti pada pemeliharaan larva kepiting (Nogami & Maeda, 1992), larva rajungan (Susanto et al, 2005), dan budidaya artemia (Villamil et al, 2003), pada pemeliharaan rainbow trout (Brunt & Austin, 2005;Brunt et al, 2007), ikan karper (Labeo rohita) (Kumar et al, 2006;Banerjee et al, 2007), pada pematangan gonad ikan hias (Ghosh et al, 2007), pemeliharaan ikan nila (Tilapia nilotica) (Aly et al, 2008, Wang et al, 2008, larva ikan sebelah (flatfish) Senegalese sole (Makridis et al, 2008, Diaz-Rosales et al, 2009, larva gilthead sea bream (Sparus aurata, L) (Suzer et al, 2008, Avella et al, 2010, dan budidaya ikan kakap putih (seabass) (Rengpipat et al, 2008), pada budidaya abalon (Macey & Coyne, 2005;Iehata et al, 2010), dan pada budidaya ikan salmon , pada pemeliharaan larva lobster (Daniels et al, 2010).…”