Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Abstract

Placental Extract (PE) has been used as a folk remedy in Asian countries. Human PE is clinically used for the treatment of skin wrinkles because it promotes skin regeneration. In this study, we found that Porcine PE (PPE) promoted collagen production in Normal Human Dermal Fibroblasts (NHDFs). Treatment of NHDFs with PPE at concentrations of 12.5-100 μg/mL enhanced their proliferation and secretion of procollagen C peptide. We also found that expression of intracellular Matrix Metalloproteinase-9 (MMP-9) in PPE-treated NHDFs was decreased in a dose-dependent manner. These results indicate that PPE might affect facial wrinkling partly by enhancing fibroblast proliferation and collagen secretion while inhibiting MMP-9 expression in NHDFs.

Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2

“…We previously reported that HGFs shows a similar cell proliferation tendency under the same P-placenta concentration 8) . Yoshikawa et al reported that 0-100 g/ml Pplacenta elevated the proliferation of human skin fibroblasts 18) .…”

Case Report: Improvement of Long Lasting Hyperpigmentation by Aqueous Human Placenta Extract (Reju Growth Factor, RGF&#174) Treatment

“…The placenta extract is scientifically proven skin-whitening effect [7] [11] [13] and reduces oxidative stress. It also induces fibroblast proliferation and collagen synthesis [10]. It could inhibit melanin synthesis and also regulate antioxidant genes and might protect the skin against oxidative stress [14] [15] [16].…”

Effects of Porcine Placenta Extract Ingestion on Ultraviolet B-induced Skin Damage in Hairless Mice

“…Yoshikawa et al . ( Yoshikawa et al ., 2013 ) demonstrated that oral PPE treatment may be used to improve skin wrinkles. Oral PPE treatment in postmenopausal women leads to a decrease in the width of wrinkles.…”

“…A recent study has shown that PPE drives skin fibroblasts into the S phase and increases the basic fibroblast growth factor level ( Pal et al ., 2002 ). It significantly increases collagen production in dermal fibroblasts, suggesting that it has a stimulatory effect on collagen production ( Yoshikawa et al ., 2013 ). Skin collagen has a central role in the mechanical strength of skin, as a component of the extracellular matrix.…”