Effects of Porcine Placenta Extract Ingestion on Ultraviolet B-induced Skin Damage in Hairless Mice

Abstract

The aim of our study was to evaluate the potential benefits of an oral supplement containing porcine placenta extract (PPE) on skin parameters related to cutaneous physiology and aging. PPEs were administered orally to hairless mice for 12 wk. The effects of oral PPE administration on skin water-holding capacity and Transepidermal Water Loss (TEWL) were similar to those of oral collagen (HYCPU2) administered as a positive control. Magnified photographs and replica images showed a reduction in UVB-induced wrinkle formation after collagen and PPE treatments. PPE treatments ameliorated the thicker skin surface that results from UVB exposure, based on a histological examination of skin tissue. The groups that were orally administered PPE (0.05%, OL; 0.1%, OH group) showed significantly reduced Matrix Metaloproteinase-2 (MMP-2) mRNA expression levels compared with the UVB control (Con), by 33.5% and 35.2%, respectively. The mRNA expression of another collagen-degrading protein, MMP-9, was also significantly lower in the groups that received oral administration of PPE (especially in the OH group) than in the control group. Additionally, oral administration of PPE significantly upregulated tissue inhibitor of metalloproteinase-1 (TIMP-1) and -2 mRNA expression levels compared with expression levels in the control group (p<0.05). This indicates that orally administered PPE activated the expression of Timp-1 and -2, inhibitors of MMP, which is responsible for collagen degradation in skin. Taken together, we propose that long-term oral administration of PPE might have a beneficial effect with respect to skin photo-aging.

Anti-Photoaging Effect of Jeju Putgyul (Unripe Citrus) Extracts on Human Dermal Fibroblasts and Ultraviolet B-induced Hairless Mouse Skin

“…Among several MMPs, MMP-1 plays a role in decomposing ECM dermal collagen that is mainly composed of type-1 collagen [ 33 ]. It is well known that natural plants have anti-photoaging effects through increasing collagen production by inhibition of MMP [ 34 , 35 ]. In this study, putgyul extracts decreased MMP-1 production in HDFs in a dose-dependent manner.…”

Porcine Placenta Extract Reduced Wrinkle Formation by Potentiating Epidermal Hydration

“…A previous study has shown that the injection of human placenta extract into wound boundaries accelerated decrease in wound size [6]. Moreover, oral administration of PPE reportedly reduces UVB-induced wrinkle formation in mice, along with the downregulation of MMP-2 mRNA expression [7]. In addition, studies using skin cells have revealed that human placenta extract accelerates keratinocyte and fibroblast proliferation [8] [9].…”

The effect of purple mangosteen (Garcinia mangostana) peel extract on collagen fiber in male Wistar rats after Ultraviolet-B (UV-B) exposure

“…12 Anthocyanin which also contained in mangosteen peel extract is a powerful antioxidant because of its high reactivity as an electron donor and can stabilize unpaired electrons. 13 dermis compared to controls, but not statistically significant when compared with the placebo group. The insignificant difference in the mean of collagen in rat given the mask of mangosteen peel extract compared to placebo may occur probably because the dosage of mangosteen skin extracts present in the mask is insufficient to cause a statistically…”