Anti-Photoaging Effect of Jeju Putgyul (Unripe Citrus) Extracts on Human Dermal Fibroblasts and Ultraviolet B-induced Hairless Mouse Skin

Abstract

Ultraviolet (UV) radiation stimulates the expression of matrix metalloproteinases (MMPs) and inflammatory cytokines. These signaling pathways participate in the degradation of the extracellular matrix and induce inflammatory responses that lead to photoaging. This study evaluated the antioxidant activity and the effect on MMPs and procollagen of putgyul extract in vitro. The anti-photoaging activity of putgyul extracts was estimated in vivo using hairless mice (HR-1). The putgyul extracts reduced MMP-1 production and increased the content of procollagen type I carboxy-terminal peptide in human dermal fibroblasts. Ultravilot-B (UVB)-induced expression of inflammatory cytokines and MMPs was detected in mice, and putgyul extracts suppressed the expression. These results suggest that putgyul extract inhibits photoaging by inhibiting the expression of MMPs that degrade collagen and inhibiting cytokines that induce inflammatory responses. The mouse model also demonstrated that oral administration of putgyul extracts decreased wrinkle depth, epidermal thickness, collagen degradation, and trans-epidermal water loss, and increased β-glucosidase activity on UVB exposed skin. Putgyul extract protects against UVB-induced damage of skin and could be valuable in the prevention of photoaging.

Hydrangea serrata (Thunb.) Ser. Extract Attenuate UVB-Induced Photoaging through MAPK/AP-1 Inactivation in Human Skin Fibroblasts and Hairless Mice

“…Studies have shown that certain dietary factors can improve the signs of skin aging [9,25]. Accordingly, the attention of the cosmetic industry has recently focused on numerous crude herbal extracts to prevent skin aging [26,27]. In this report, and for the first time, we aimed to determine the anti-photoaging activity of WHS and elucidate the associated molecular mechanisms.…”

Anti‑photoaging effect of fermented agricultural by‑products on ultraviolet B‑irradiated hairless mouse skin

“…RT-qPCR was performed by modifying a previously published protocol (25) to determine the effect of FRB, FSB and FSC (40 mg/kg) on MMPs (MMP-2,9,3 and 13) and inflammation-associated cytokines including TNF-α, COX-2, iNOS, IL-6 and IL-1β. Total cellular RNA was extracted from skin tissue using the phenol-chloroform method using RNAzol (Tel-Test, Inc., Friendswood, TX, USA).…”

“…In addition, the increase in matrix metalloproteinase (MMP) activity in response to ROS production results in the destruction of the structure and function of the extracellular matrix (ECM) via collagen degradation (24). UV exposure also stimulates the production of cyclooxygenase-2 (COX-2), which aggravates skin inflammation by catalyzing the synthesis of prostaglandin E2 and inducing the expression of inducible nitric oxide synthase (iNOS), which may result in skin inflammation (25).…”

Chemical Composition of a Novel Distillate from Fermented Mixture of Nine Anti-Inflammatory Herbs and Its UVB-Protective Efficacy in Mouse Dorsal Skin via Attenuating Collagen Disruption and Inflammation

“…The mechanisms of UV-induced skin damage involve inflammation by inflammatory cytokines and collagen degradation by collagenases [ 6 ]. Among the inflammatory cytokines, proinflammatory cytokines such as interleukin (IL)-1β and tumor necrosis factor alpha (TNF-α) stimulate the generation and accumulation of ROS and, furthermore, increase the expression of matrix metalloproteinases (MMPs), which accelerate structural and functional destruction of the extracellular matrix (ECM) and lead to collagen disruption [ 7 ].…”