Effect of pomiferin administration on kidney ischaemia-reperfusion injury in rats

Abstract

The aim of the study was to analyse protective effects of different doses of pomiferin in therapy of reperfusion injury. Rats were randomly divided into five groups (n=10). One group was intact. Three medicated groups and one placebo group were subjected to ischaemia and reperfusion of the left kidney. Pomiferin was administrated by single gastric gavage in 2 ml of 0.5% Avicel solution in doses of 5, 10 and 20 mg/kg. The placebo group was given only Avicel solution. On day 15, all the animals were exsanguinated and the reperfused kidneys were recovered. Selected biochemical markers were assessed in blood: antioxidative enzymes, total antioxidative capacity, malondialdehyde, creatinine, urea and uric acid. Creatinine, urea and total proteins were analysed in urine and 24-hour diuresis was recorded. The kidney tissue samples were used for histopathological examination.The results confirmed the expected protective effects of pomiferin. Pomiferin supported defensive reactions of the body against free radicals (increased levels of superoxide dismutase, total antioxidative capacity), decreased lipid peroxidation (decreased malondialdehyde) and contributed to the recovery of kidney functions (creatinine and urea in blood). The best biochemical and histopathological results were achieved after pomiferin administration in the dose of 5 mg/kg.

Electrochemical Characterization and Quantification of the Strong Antioxidant and Antitumor Agent Pomiferin

“…Its antioxidant activity, usually determinate by nonelectrochemical methods, is comparable to well know antioxidant activity of compounds such as ascorbic acid, Trolox (the main component of vitamin E) and the synthetic BHT (butylated hidroxytoluene) [13]. Besides this property, pomiferin was shown to have highly potent antimicrobial activity [14], antidiabetic effects in alloxan-induced diabetes mellitus [15] and cytotoxic activity towards human cholangiocarcinoma cells (HuCCA-1) [9]. More recently [16], pomiferin showed potential inhibitory activity in histone deacetylase (HDAC) enzyme assay and exhibited growth inhibitory activity on five human (kidney, lung, prostate, breast, melanoma and hepatocytes) tumor cell lines and more sensitive inhibitory activity on the HCT-15 colon tumor cell line.…”

Effects of Xenobiotics on Total Antioxidant Capacity

“…Another statin, atorvastatin, has also been found to enhance plasmatic TAC and decrease oxidative stress in coronary artery disease dislipidemic patients (Buyukhatipoglu et al , 2010). Natural flavonoids like pomiferin also protected rats against ischemic-reperfusion kidney injury by decreasing lipid peroxidation and GPx, and improving the SOD and TAC levels (Bartošíková et al , 2010). …”

Antioxidant Activity of Osage Orange Extract in Soybean Oil and Fish Oil during Storage

“…Osajin and pomiferin were shown to have anti-inflammatory (Abourashed et al, 2015), antidiabetic (Bartošíková et al, 2008) and cardioprotective (Florian et al, 2006;Nečas et al, 2006) activities. Pomiferin has also been shown to have anticancer (Yang et al, 2011) and antiulcer (Bozkurt et al, 2017) activities, to promote recovery of kidney functions (Bartošíková et al, 2010), and to inhibit intracellular oxidative stress (Abourashed et al, 2015). Other bioactive components in Osage orange fruit extracts that have been identified include scandenone, auriculasin (Kupeli et al, 2006) and other prenylated flavonoids (Orazbekov et al, 2018).…”