Effect of polymorphisms in the ABCG2, LEPR and SCD1 genes on milk production traits in Holstein cows

Abstract

This study was performed to investigate the association between polymorphisms in the ABCG2 (ATPbinding cassette sub-family G member 2), LEPR (leptin receptor) and SCD1 (stearoyl-coenzyme A desaturase 1) genes and milk production traits in Holstein dairy cows in Iran. The analysis was conducted on 816 lactations from 408 Iranian Holstein cows. Genotyping was carried out using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique. The results of the association study revealed that cows with genotype AC of the ABCG2-Y581S single nucleotide polymorphism (SNP) had lower breeding value for milk yield compared with the AA genotype, but showed significantly higher fat and protein percentages. Cows that were homozygous for allele T at the LEPR-T945M locus had higher breeding value for fat yield than those that were homozygous for the C allele. Regarding the SCD1-A293V SNP, cows with genotype AV produced a higher 305-day milk yield in comparison with cows with the VV genotype. Furthermore, cows that were homozygous for allele V showed a significantly higher protein percentage compared with AA and AV genotypes. The results of this study suggest that these SNPs have the potential to be used in programmes based on genomic selection in Iranian dairy herds. ______________________________________________________________________________________

Variation in the Genetic Effects of ABCG2, Growth Hormone and Growth Hormone Receptor Gene Polymorphisms on Milk Production Traits in Egyptian Native, Holstein and Hybrid Cattle Populations

“…The potential use of genetic polymorphism, particular polymorphism of ABCG2, GH, and GHR genes in some cattle breeds as functional candidates for production traits have been documented in several reports (Lucy et al, 1998;Zhou et al, 2005;Hadi et al, 2015;Ghombavani et al, 2016). In this study we described the use of these markers with three cattle populations, Egyptian native (Baladi), Holstein Friesian and Hybrid cattle, to find out if these markers can be used with these different cattle population for selection and improvement of milk quality and milk yield.…”

“…Mutations in the GHR gene alter GH functions by changing the protein's ability to bind to this receptor on target cells (Maskur and Arman 2014). Several reports documented the association between milk yield and composition in cattle and the polymorphism in ABCG2 (Mousavizadeh et al 2013;Ghombavani et al, 2016), GH (Ge et al, 2003;Kim et al, 2004;Mullen et al, 2010;Komisarek et al, 2011;Mullen et al, 2011), and GHR genes (Blott et al, 2003;Maj et al, 2005).…”

Single Nucleotide Polymorphisms, Gene Expression and Economic Evaluation of Parameters Associated with Mastitis Susceptibility in European Cattle Breeds

“…Komisarek et al [53] reported a significant effect of ABCG2 gene polymorphisms on estimated breeding values for milk fat production traits, calving-to-first insemination interval, and non-return rate in Polish Holstein-Friesian cattle. Genetic effects of ABCG2 polymorphism on milk production traits were also reported in the Holstein cattle [54,55]. Zhang et al [28] elicited that SLC11A1 gene polymorphisms are not associated with somatic cell score and milk yield in Chinese Holstein.…”

Identification of Genetic Variants in ABCG2 Gene Influencing Milk Production Traits in Dairy Cattle

“…Genotypic frequencies of AA, AC and CC were 0.83, 0.15 and 0.02 whereas the allelic frequencies for A and C allele were 0.91 and 0.19, respectively (Table 5). Soltani-Ghombavani et al (2016) in Iranian Holstein cattle also observed a much higher frequency of A allele compared with C allele (97% vs. 3%) of the ABCG2-Y581S locus. Development of crossbred cattle started, by crossing Tharparkar (T) as a Zebu breed and three exotic breeds, namely, Holstein Friesian (H), Brown Swiss (B) and Jersey (J) at National Dairy Research Institute, Karnal.…”