Single Nucleotide Polymorphisms, Gene Expression and Economic Evaluation of Parameters Associated with Mastitis Susceptibility in European Cattle Breeds

Ateya, Ahmed; Ibrahim, Samer S.; Al‐Sharif, Mona

doi:10.3390/vetsci9060294

Cited by 9 publications

(10 citation statements)

References 70 publications

(86 reference statements)

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“…In addition, discovered a link between mastitis resistance and β-defensin gene polymorphisms ( Tolone et al ., 2016 ). Investigated SNPs in immunological ( SELL, ABCG2, SLC11A1 , and FEZL ) and antioxidant ( SOD1, CAT, GPX1 , and AhpC/TSA ) genes linked with mastitis risk in Holstein and Brown Swiss dairy cows were investigated ( Ateya et al ., 2022 ). The CD14 gene polymorphism and its link to mastitis was reported ( Kumar et al ., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…(2012 ) showed that mastitis-infected bovine mammary tissues had considerably greater levels of A2M mRNA and protein expression than did healthy tissues. According to Ateya et al . (2022 ), compared to tolerant cows, mastitic Holstein and Brown Swiss dairy cows exhibited significantly greater levels of SELL, SLC11A1 , and FEZL gene expression.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Individual genomic loci, transcript level and biochemical profile of immune and antioxidant markers associated with genetically identified bacterial mastitis in Shami goats in Egypt

Ebissy,

Darwish,

Hafez

et al. 2024

Open Vet J

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Background: Mastitis in goats is unquestionably a grave concern, with far-reaching implications for both animal well-being and productivity, while also presenting a potential threat to public health. Aim: The study aimed to compare between culture methods and multiplex PCR (m-PCR) in detection of the most 3 common mastitis causing pathogens (Staphylococcus aureus, E. coli, Streptococcus spp) and investigating the gene expression, single nucleotide polymorphisms (SNPs), serum concentrations of immunological and antioxidant indicators linked to mastitis in Shami goats. Methods: One hundred Shami does (fifty Shami goats with clinical mastitis and fifty normal goats taken as control group). The culture methods and m-PCR analysis were used to find the bacteria in the milk samples.Blood samples were obtained in order to assess some hemato-biochemical parameters, detect SNPs, and determine the expression of certain immunological and antioxidant indicators in the genes. Results: Culture method detected the pathogens causing mastitis in 90% of the milk samples, but m-PCR detected them in 100% of the milk samples. SNPs linked to mastitis resistance/susceptibility in examined does were detected through DNA sequencing of immunological and antioxidant indicators. Magnitude of gene expression varied significantly between the resistant and mastitis affected groups. Significant (P˂0.05) elevations were noticed in WBCs count, mainly neutrophils count, serum levels of BHB, NEFA, triglycerides, LDL-C, AST, ALT, ALP, creatinine, total protein, globulin, Ca, K, GPx, MDA, acute phase proteins and cytokines in mastitis affected does as compared to control. While RBCs count, PCV%, lymphocytes count, serum concentration of glucose, cholesterol, HDL-C, albumin, Na, Cl, P, GSH, SOD, catalase significantly (P˂0.05) diminished in mastitis affected does compared to healthy ones. APPs and pro-inflammatory cytokines scored high sensitivities and NPVs but TNF-α and SAA had the highest percentages of increase. Conclusion: The study confirmed that m-PCR is the most sensitive method for bacteria identification (S. aureus, E. coli and Strept. spp.) while SNPs in antioxidant and immunological genes may be important genetic indicators for mastitis risk or resistance in Shami does. In order to establish an effective management plan and forecast the most sensitive risk time for illness onset, gene expression profiles of the tested genes may also be employed as proxy biomarkers. TNF-α and SAA may be precious indicators for detection of caprine mastitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Individual genomic loci, transcript level and biochemical profile of immune and antioxidant markers associated with genetically identified bacterial mastitis in Shami goats in Egypt

Ebissy,

Darwish,

Hafez

et al. 2024

Open Vet J

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“…Using PCR-DNA sequencing for the immunological ( TLR4 , IL10 , TLR7 , NCF4 , TNF-α , and LITAF ) genes, there were changes in the nucleotide sequence between endometritis-affected cows and healthy ones. In addition, using PCR-DNA sequencing of immunological ( SELL , ABCG2 , FEZL , and SLC11A1 ) markers from the investigated Holstein and Brown Swiss dairy cows, nucleotide sequence alterations in the form of SNPs related to mastitis tolerance/susceptibility were found [ 44 ]. In cows, the LTF gene was linked to mastitis resistance [ 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes

Ateya,

Safhi,

El-Emam

et al. 2023

Veterinary Sciences

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The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers’ nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy.

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“…The morphology and anatomy of cow udder has long been a subject for curious selection to improve the efficiency of milk production 5 . In addition, the teats of dairy animals are an important part of the udder, which is attached with a milking cluster and meantime, serves the roles of both a valve regulating the outflow of milk and of a natural barrier for exogenous infections 6 , 7 , teat shape and dimensions have no less emphasis in that regard 5 . Poor udder and teat conformations form a management challenge for dairy cow commercial producers.…”

Section: Introductionmentioning

confidence: 99%

“…Most dairy cattle breeding objectives worldwide have focused exclusively on production traits paying no attention to the severe deterioration in production properties caused, mainly by the unfavourable phenotypic and genetic correlations between linear type and milk production traits 37 . On the other side, there are genomic regions, that involve quantitative traits locus (QTLs), candidate genes and significant single-nucleotide-polymorphisms (SNPs) related to the udder and teat traits in dairy cattle 6,26,38 .The present study aimed to evaluate udder and teat characteristics in order to predict their relationship with milk production traits. The effects of udder shape, sire, parity, inbreeding and age at first calving on udder and teats measurements were also studied, with a spotlight on the genetic background of concerning traits.…”

mentioning

confidence: 99%

Prediction of some milk production traits using udder and teat measurements with a spotlight on their genetic background in Friesian cows

Saleh,

Easa,

EL-Hedainy

et al. 2023

Sci Rep

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The aggregate udder shape (Bowl, Round, Cup), udder measurements (udder width, UW, udder front depth, UFD, udder rear depth, URD, udder levelness, ULV, udder heights, UH) and teat measurements (teat diameters, TD, front teat length, FTL, rear teat length, RTL, distance between front teats, DFT and distance between rear teats, DRT) were measured on 1300 Friesian cows located in a commercial farm under subtropical conditions (Egypt) to appraise udder and teats status and to evaluate the possible relationships with some milk production characteristics in conjunction with udder shape, age at first calving, sire and inbreeding effects on udder morphological traits and milk production ability. For such an available sample size, parity had affected (P < 0.01) UFD, TD, FTL and RTL. In addition, udder shape affected (P < 0.01) UW, UFD, URD, ULV and DFT. None of the other studied factors affected milk production traits. The bowl udder shape (P < 0.01) yielded a high total milk yield (3267.19 kg), adjusted milk yield (2443.01 kg) and lactation length (480.70 d) compared to other udder shapes. The genetic correlations of UW with total milk yield and persistency were strongly positive (0.86 and 0.93, respectively). However, strong negative genetic correlations were found between UW with peak milk yield and lactation length (− 0.92 and − 0.80, respectively), between RTL with peak milk yield (− 0.92) and DRT with persistency (− 0.79). As found from the stepwise multiple regression, UW and URD can be used as good indicators for predicting milk yield and lactation length. Additionally, this study spotlights the genetic background of udder characteristics based on reliable studies and the QTL database for cattle as a first step toward applying this knowledge side by side with phenotypic traits to improve the productivity of the Holstein breed under subtropical conditions.

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Single Nucleotide Polymorphisms, Gene Expression and Economic Evaluation of Parameters Associated with Mastitis Susceptibility in European Cattle Breeds

Cited by 9 publications

References 70 publications

Individual genomic loci, transcript level and biochemical profile of immune and antioxidant markers associated with genetically identified bacterial mastitis in Shami goats in Egypt

Individual genomic loci, transcript level and biochemical profile of immune and antioxidant markers associated with genetically identified bacterial mastitis in Shami goats in Egypt

DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes

Prediction of some milk production traits using udder and teat measurements with a spotlight on their genetic background in Friesian cows

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