Variation in the Genetic Effects of ABCG2, Growth Hormone and Growth Hormone Receptor Gene Polymorphisms on Milk Production Traits in Egyptian Native, Holstein and Hybrid Cattle Populations

Abstract

The aim of the present study was to evaluate the effect of polymorphisms in the ATP-binding cassette subfamily G member 2 (ABCG2), growth hormone (GH) and growth hormone receptor (GHR) genes on 305-day yields and milk traits in native Egyptian cattle (Baladi), Holstein Friesian cattle and hybrid cattle using DNA sequencing and Restriction Fragment Length Polymorphisms (RFLPs). A total of 148 cows were selected from the three animal populations. The ABCG2-HhaI-AA genotype was significantly associated with an increased milk lactose percentage and reduced milk yields in Baladi cows. The GH-MSP1-heterozygous genotype (GH) showed significant associations with SNF, protein and lactose percentages in the hybrid cows. Fewer TG repeats were found in the 5' untranslated region of GHR in Baladi cows, which could be used for marker-assisted selection to protect against the lower milk yields observed in this population. Additionally, GHR-MSP1-VL, can be associated with higher SNF, fat, protein and lactose percentages in hybrid cows, as well as lower milk yields in Baladi cows. We concluded that the three cattle populations have their own genetic identities in relation to genetic markers, which can be used for marker-assisted selection to improve milk traits in these three cattle populations.

Determination of the association of <i>GHR</i>/<i>AluI</i> gene polymorphisms with milk yield traits in Holstein and Jersey cattle raised in Turkey

“…Despite this, some authors (Aggrey et al, 1999;Banos et al, 2008;Li-Juan et al, 2009;Kiyici et al, 2019) observed that GHR/AluI polymorphisms had a significant effect on milk yield traits in Holstein cows. The results were also in the study conducted by El-Nahas et al (2018), who stated that there was a significant relationship between GHR/AluI and PC. By contrast, in a study about a gene related to GHR, Hartatik et al (2015) determined the association between GH gene polymorphism on FC (%) in Friesian Holstein cow groups from New Zealand and Australia.…”

“…As shown in Table 1, the reasons for the differences in the quantitative characteristics of Holstein cows raised in two different herds can be attributed to the fact that they were raised in different regional conditions of Turkey and have different management feeding regimes and different environmental conditions. Moreover, El-Nahas et al (2018) stated that the impact of GH genes on 305-DMY and FC in Holstein cows was not significantly important. Kiyici et al (2019) reported that no significant relationships were observed between GH/TaqI polymorphism and milk fat and protein content.…”

“…Grochowska et al (2001) determined that the milk yield in Simmental cattle was positively related to the LV genotype at the GH/AluI site. Moreover, El-Nahas et al (2018) reported that GHR/Msp1 was associated with the different milk composition characteristics in Baladi cattle. In a recent study, Kiyici et al (2019) also reported that the cows with the LL genotype had higher milk yields than Turkish Holstein cows with the LV genotype in agreement with our findings.…”

“…The following re-parameterized equations were applied: a = (AA − GG)/2, d = AG − (AA + GG)/2, and α = a + d(q − p), where q and p represent the frequencies of alternative alleles. While the same capital letters used together represent the homozygous genotypes, the use of different letters stands for the heterozygous genotype in based on Falconer and Mackay (1996).…”

Evaluation of candidate genotype of GH gene associated with growth, production and reproduction traits in Dairy Cows

“…Similar observations corresponding to Sahiwal cattle were also reported as TT(1.00) in Bali, CC(0.16), CT(0.35), TT(0.49) in Grati, CC(0.05), CT(0.30), TT(0.65) in Pesisir, CC(0.23), CT(0.22), TT(0.55) in Madura, TT(1.00) in Aceh cattle breeds (Putra et al., 2013). A similar trend was found in Hariana CT(0.41), TT(0.59); Sahiwal CT(0.12), TT(0.88); Gir CT(0.24), TT(0.76) and Kankrej cattle CT(0.18), TT(0.82) (Sodhiet al, 2007) However, a higher frequency of the CC genotype was observed as CC(0.77), CT(0.23) in Jersey, CC(0.77), CT(0.21), TT(0.02) in Holstein, CC(0.76), CT(0.22), TT(0.02) in Polish Friesian cattle; CC(0.75), TT(0.25) in Baladi cattle, CC(0.79), CT(0.16), TT(0.05) in Hybrid and CC(0.73), CT(0.27) in Holstein Friesian (Nahas et al, 2018).…”