Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows

Abstract

Effect of polymorphic variants of growth hormone (GH), β lactoglobulin (β LG), and Pit 1 genes on milk yield was analyzed in a Holstein herd. Genotypes of the cows for these genes were determined by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) method. Allele frequen cies were 0.884 and 0.116 for L and V variants of GH, 0.170 and 0.830 for A and B variants of Pit 1, and 0.529 and 0.471 for A and B variants of β LG, respectively. GLM procedure of SAS software was used to test the effects of these genes on milk yield. Results indicated significant effects of these genes on milk yield (P < 0.05). Cows with LL genotype of GH produced more milk than cows with LV genotype (P < 0.05). Also, for Pit 1 gene, animals with AB genotype produced more milk than BB genotype (P < 0.05). In the case of β LG gene, milk yield of animals with AA genotype was more than BB genotype (P < 0.01). Therefore, it might be concluded that homozygote genotypes of GH (LL) and β LG (AA) were superior compared to heterozygote genotypes, whereas, the heterozygote genotype of Pit 1 gene (AB) was desirable.

Effect of casein genes - beta-LGB, DGAT1, GH, and LHR - on milk production and milk composition traits in crossbred Holsteins

“…The variant AA of the beta-LGB gene had a very large positive effect on MY. This result is consistent with those of many previous studies (e.g., Heidari et al, 2012). The variant AB of the kappa-casein gene showed a very large effect on the percentages of fat, protein, solids-not-fat, and total solid.…”

“…These results differ from those of Heidari et al (2012), who found that the frequency of allele L was very high in a study of Holstein dairy cattle.…”

“…Some candidate genes were found to have significant effects on the traits, including the casein gene family and the betalactoglobulin (LGB) (Boettcher et al, 2004;Comin et al, 2008), acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) (Kuehn et al, 2007;Nowacka-Woszuk et al, 2008;Signorelli et al, 2009), growth hormone (GH) (Pawar et al, 2007;Mullen et al, 2011;Heidari et al, 2012;Krasnopiorova et al, 2012), and luteinizing hormone receptor (LHR) genes (Hastings et al, 2006). There is strong evidence that a group of genes may be used as markers for selection programs.…”

Polymorphisms of LEP, LGB and PRLR in water buffalo

“…The Korean cattle, Bos taurus, is a breed native to the Korean peninsula and the Japanese islands, and is considered to descend from a breed of European cattle (Seong et al, 2012) In a previous study, Leptin (LEP), beta-lactoglobulin (LGB) and Prolactin receptor (PRLR) were shown to be genes that are associated with milk production traits in cattle (Ruheena, et al, 2011;Shi, et al, 2011;Heidari, et al, 2012;Tanpure, et al, 2012). LEP, the fat derived protein hormone, has a variety of physiological functions such as regulation of feed intake, energy balance, and fertility and immune functions (Tanpure et al, 2012).…”

Investigation of the promoter polymorphisms of the growth hormone (GH1), growth hormone receptor (GHR), insulin-like growth factor (IGF-I), and prolactin (PRL) genes and the correlation between gene expression and milk yields in Holstein cattle raised in Central Anatolia

“…On the other hand, Heidari et al (13) demonstrated that individuals with a VV genotype had milk yields higher than those of individuals of other genotypes, but also indicated that this difference was statistically insignificant. In their study, in which they investigated the correlation between the GH1-MspI polymorphism and milk yield in Holstein cattle, Zhou et al (14) determined that individuals with an AA genotype had a higher milk yield than individuals with an AB genotype, and suggested that the GH1-MspI polymorphism could be used for the improvement of milk yield traits.…”