Polymorphisms of LEP, LGB and PRLR in water buffalo

Abstract

:The polymorphisms of several genes including Leptin (LEP), beta-lactoglobulin (LGB) and Prolactin receptor (PRLR) have been shown to affect milk composition traits in dairy cattle. But, the effects of these polymorphisms on the milk traits of Philippine water buffalo are still unclear. In the Philippines, buffalo are the major milk producers most of which are the Philippine carabao (PC), the American Murrah Buffalo (AMB) and Bulgarian Murrah Buffalo (BMB). The LEP, LGB and PRLR genes are considered to be associated with milk production traits. The objective of the present study was to identify the single nucleotide polymorphisms (SNPs) in the LEP, LGB and PRLR genes of PC, AMB and BMB and to investigate the effect of the SNPs on milk production traits in these buffalo. Genetic polymorphisms were screened by DNA sequencing and 12 SNPs were detected in BMB; 5 SNPs were in LEP exon3 region (G14227A, G14343A, T14502C, C14526T, G14603A); 5 SNPs were in LGB exon 2 region (G1861C, A1900G, G1901T, T1948C, G1949A); 2 SNPs were in PRLR exon 6 (T59047C, T59109C). Also, 12 polymorphism sites between cattle and buffalo were identified. Our analysis of the association between SNPs and milk production traits should be useful in future studies of buffalo breeding to improve lactation performance.

Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels

“…PCR-RFLP pattern using Eco91I of the target fragment showed that all the investigated buffaloes had CC genotype which was confirmed by direct sequencing. In agreement with the current results, some investigators confirmed the CC genotype as the only detected genotype among several river buffalo breeds [3,[8][9][10][11][12]. But in cattle, the frequencies of CT and TT genotypes were 34% and 8% (Liefers et al [4]), 47.03% and 9.13% [7], 38.8% and 2.4% [5], and 33% and 6% [6], respectively.…”

“…The alleles of G140A, G263A, and G379A SNPs in addition to 21 SNPs were found between in the leptin gene in the sequenced fragment of the Egyptian buffalo and the other buffalo breed records for the same gene in GenBank. The T allele of C32T intronic SNP was unique and was detected in Egyptian buffalo within this investigation only and was not detected in any buffalo records in Gen-Bank or previous studies on river buffalo [3,[8][9][10][11][12].…”

“…Both of the Italian [8] and Egyptian buffaloes were polymorphic for G140A and G379A SNPs, but in contrast to Egyptian buffaloes, the Italian buffaloes were monomorphic for G263A SNP and polymorphic for G278A SNP. The Philippine buffaloes [9] had the two alleles of G263A and G379A SNPs exactly like the Egyptian breeds.…”

“…Orrú et al [8] discovered 1 SNP in exon 2, 5 SNPs in exon 3, and 8 intronic SNPs by analysis; the sequence of 2 fragments represents exons 2 and 3 and part of intron 1 that covers all the leptin gene coding sequence in 32 Italian River Buffalo and 2 Egyptian river buffalo, while Seong and Kong [9] investigated the sequence of leptin gene-exon 3 in 20 American Murrah Buffalo and 350 Bulgarian Murrah Buffalo and found 3 SNPs between these populations.…”

Polymorphisms in the exon-3 of leptin gene using tetra-primer ARMS-PCR in Egyptian buffalo

“…However, this polymorphism was found in the heterozygous state in only one sample of Italian buffalo, whereas the other animals showed a GG homozygote genotype (Orrù et al, 2007). The existence of an A allele was confirmed by DNA sequencing of 390 Murrah and Philippine Carabao buffaloes, with an allele frequency of 0.18 (Seong and Kong, 2012). Also, Kale et al, 2013 detected the same variation by SSCP in Murrah, Surti and Bhadawari, with a high allele frequency of 0.3.…”