Effect of NAD+-dependent formate dehydrogenase on anaerobic respiration of Shewanella oneidensis MR-1

Mordkovich, N. N.; Воейкова, Т. А.; Novikova, L. M.; Смирнов, И. А.; Ильин, В. К.; Soldatov, P. E.; Tyurin-Kuz'min, A. Yu.; Smolenskaya, T. S.; Вейко, В. П.; Шакулов, Р. С.; Дебабов, В. Г.

doi:10.1134/s0026261713040061

Cited by 15 publications

(20 citation statements)

References 14 publications

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“…It is possible to assume that a decrease in the con tent of recombinant SEB, SEH, and SAV proteins in the periplasmic fraction of S. oneidensis MR 1 trans formants is a consequence of both an insufficiently successful expression of the studied genes in the S. oneidensis MR 1 cells under the control of selected promoter-operator region (although it contradicts previously obtained data [12,35]) and a decrease in the efficiency of translocation of the recombinant pro proteins in the periplasmic space. Analysis of the proteins of cytoplasmic fraction of the S. oneidensis MR 1/pLSEB/pLSEH/pLSAV/pRHKm transfor mant strains by the method of polyacrylamide gel elec trophoresis under denaturing conditions (Fig.…”

Section: Resultsmentioning

confidence: 61%

Investigation of protein translocation Sec-system with heterologous gene expression in Shewanella oneidensis MR-1 bacterium cells

Mordkovich

Okorokova

Вейко

2015

Appl Biochem Microbiol

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A comparison of the primary structures of the protein translocation Sec system proteins in the Shewanella oneidensis MR 1 and Escherichia coli bacteria was carried out. The process of translocation of recombinant pro enterotoxins (SEB and SEH) from Staphylococcus aureus and pro streptavidin (SAV) from Streptomyces avidinii in the S. oneidensis MR 1 and E. coli cell periplasm was studied. It was demonstrated that these marker proteins are transferred into the periplasmic space of the S. oneidensis MR 1 transformant strain cells. The identity of N terminal amino acid sequences of mature recombinant SEB, SEH, and SAV proteins (generated during post translation proteolysis of leader peptide by the Sec system both in E. coli and S. oneidensis MR 1) was established.

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Section: Resultsmentioning

confidence: 61%

Investigation of protein translocation Sec-system with heterologous gene expression in Shewanella oneidensis MR-1 bacterium cells

Mordkovich

Okorokova

Вейко

2015

Appl Biochem Microbiol

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“…However, it has also been described that the introduction of NAD-dependent FDH in Moraxella sp. increases the current density in MFC (Mordkovich et al 2013).…”

Section: Catabolic and Electron-transport Pathwaysmentioning

confidence: 95%

Understanding and engineering electrochemically active bacteria for sustainable biotechnology

Hirose

Kasai

Koga

et al. 2019

Bioresour. Bioprocess.

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Electrochemically active bacteria (EAB) receive considerable attention in sustainable biotechnology, since they are essential components in microbial fuel cells (MFCs) that are able to generate electricity from biomass wastes. EAB are also expected to be applied to the production of valued chemicals in microbial electrosynthesis systems (MESs) with the supply of electric energy from electrodes. It is, therefore, important to deepen our understanding of EAB in terms of their physiology, genetics and genomics. Knowledge obtained in these studies will facilitate the engineering of EAB for developing more efficient biotechnology processes. In this article, we summarize current knowledge on Shewanella oneidensis MR-1, a representative EAB extensively studied in the laboratory. Studies have shown that catabolic activities of S. oneidensis MR-1 are well tuned for efficiently conserving energy under varied growth conditions, e.g., different electrode potentials, which would, however, in some cases, hamper its application to biotechnology processes. We suggest that understanding of molecular mechanisms underlying environmental sensing and catabolic regulation in EAB facilitates their biotechnological applications.

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“…There are very few reports referring to recombinant expression in Shewanella currently. The heterologous expression of the udp genes from S. oneidensis MR-1 has been reported (Mordkovich et al 2012(Mordkovich et al , 2013. Besides, the promoter, tac, coupled with a synthetic flavin biosynthesis pathway from Bacillus subtilis has been shown to enhance extracellular electron-transfer (EET) rate of S. oneidensis MR-1 (Yang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1

Guo

Zhou

et al. 2018

Bioresour. Bioprocess.

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Background: Shewanella genus is famous for applications like electron transfer in microbe fuel cells and bioremediation of heavy metals through the Mtr pathway. A potential way to enhance the electron genesis ability of Shewanella is to express exogenous mtr genes via recombinant DNA technology. Thus, to design and develop expression vectors capable of replicating in Shewanella and enhance the genetic toolbox of the same is important. Result: In this study, a plasmid construct with a replication origin, repB, and pLacI promoter is reported for the first time to drive the expression of green fluorescent protein in S. oneidensis MR-1. Based on the same vector, the Mtr pathway genes mtrA, mtrC, and mtrCAB were also successfully expressed in MR-1. The recombinant strains had higher ferric reductase activity compared to the wild type. The highest enzymatic activity of 508.33 U/L in genetic Shewanella with mtrC gene is obtained, which is 1.53-fold higher than that of wild strain. The plasmids were stable up to 90 generations. Conclusion: We have demonstrated an expression system based on pLacI promoter and repB ori in Shewanella. Consequently, the combination of repB and pLacI will have great potential in Shewanella to turn on expression of different genes constitutively.

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Effect of NAD+-dependent formate dehydrogenase on anaerobic respiration of Shewanella oneidensis MR-1

Cited by 15 publications

References 14 publications

Investigation of protein translocation Sec-system with heterologous gene expression in Shewanella oneidensis MR-1 bacterium cells

Investigation of protein translocation Sec-system with heterologous gene expression in Shewanella oneidensis MR-1 bacterium cells

Understanding and engineering electrochemically active bacteria for sustainable biotechnology

Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1

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