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The mutants of Shewanella oneidensis MR 1 resistant to fosfomycin, a toxic analogue of phospho enolpyruvate, were obtained. The mutants exhibited increased reducing activity and higher rates of lactate utilization. A correlation was shown between the rates of metabolism of oxidized substrates and the rate of reduction of methylene blue, a mediator of electron transport. The mutants of S. oneidensis MR 1 may be used in microbial fuel cells for intensification of energy production from organic compounds.