Mutants of an electrogenic bacterium Shewanella oneidensis MR-1 with increased reducing activity

Abstract

The mutants of Shewanella oneidensis MR 1 resistant to fosfomycin, a toxic analogue of phospho enolpyruvate, were obtained. The mutants exhibited increased reducing activity and higher rates of lactate utilization. A correlation was shown between the rates of metabolism of oxidized substrates and the rate of reduction of methylene blue, a mediator of electron transport. The mutants of S. oneidensis MR 1 may be used in microbial fuel cells for intensification of energy production from organic compounds.

Microbial fuel cell performance for aromatic hydrocarbon bioremediation and common effluent treatment plant wastewater treatment with bioelectricity generation through series-parallel connection

“…Methylene blue (1%, w/v) was used as redox mediator in the anodic chamber initially for the development of mediated MFC (Voeikova et al . 2012), the performance of which was compared with mediator‐less MFC. The substrate used in this experiment was 1% sucrose.…”

“…Ltd.', Vatva, Gujarat, India. Screening of the isolated bacteria was carried out by examining their oxidation fermentation behaviour and later by methylene blue reduction tests (Voeikova et al 2012). The bacterial strains that were able to decolorize methylene blue more rapidly were screened for their performance in MFC.…”