Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1

Ng, I‐Son; Guo, Yanlan; Zhou, Yunli; Wu, Jhe-Wei; Tan, Shih‐I; Yi, Ying-Chen

doi:10.1186/s40643-018-0221-9

Cited by 10 publications

(7 citation statements)

References 41 publications

(42 reference statements)

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“…It is acidophilic, and can survive under high concentrations of heavy metal ions aided by a specific mechanism called extracellular electron transport (EET). The mechanism of metal reduction has been well-studied in Shewanella and the c-type cytochromes (i.e., MtrA, MtrB, MtrC, CymA, and OmcA) play a critical role in metal reduction 11 . The complex structure forming Mtr (i.e., metal-reducing) pathway functions by promoting the electron transfer from inner cell membrane to the cell surface 12 .…”

Section: Introductionmentioning

confidence: 99%

Mechanism study of photo-induced gold nanoparticles formation by Shewanella oneidensis MR-1

Huang

Chang

et al. 2019

Sci Rep

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Shewanella oneidensis MR-1, a bioelectricity generating bacterium, is broadly used in bioremediation, microbial fuel cell and dissimilatory reduction and recovery of precious metals. Herein, we report for the first time that photo induction as a trigger to stimulate gold nanoparticles (Au@NPs) formation by MR-1, with wavelength and light intensity as two key variables. Results indicated that sigmoidal model is the best fit for Au@NPs formation at various wavelengths (with R 2 > 0.97). Light intensity in terms of photosynthetic photon flux density (PPFD) critically influences the rate constant in the low-light intensity region (PPFD < 20), while wavelength controls the maximum rate constant in the high-light region (PPFD > 20). By deletion of Mtr pathway genes in MR-1, we proposed the mechanism for light induced Au@NP formation is the excitation effect of light on certain active groups and extracellular polymeric substances (EPS) on the cell surface. Also, the release of electrons from proteins and co-enzyme complexes enhance electron generation. To the best of our knowledge, this is the first-attempt to explore the effect of photo-induction on Au@NPs production by MR-1, which provides an alternative cost-effective and eco-friendly process in green chemical industry.

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Section: Introductionmentioning

confidence: 99%

Mechanism study of photo-induced gold nanoparticles formation by Shewanella oneidensis MR-1

Huang

Chang

et al. 2019

Sci Rep

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“…Many attempts have been made to promote the EET rate of S. oneidensis (Li et al, 2018; Ng et al, 2018; Yang et al, 2015a). With the aid of the auxotrophic complementation system, the important role of the menaquinone pool in EET was examined in a “build‐to‐understand” manner (Figure 5B).…”

Section: Discussionmentioning

confidence: 99%

A designed plasmid‐transition strategy enables rapid construction of robust and versatile synthetic exoelectrogens for environmental applications

Fan

Tang

Sun

et al. 2022

Environmental Microbiology

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Genetic engineering is promising to expand the application scope of exoelectrogens in energy and environmental applications and plasmid vectors, as one type of fundamental tool, are intensively applied. Antibiotics are widely utilized for plasmid selection and maintenance; however, their utilization suffers from environmental concerns on the spread of resistance genes, elevated costs, inevitable genotypic instability and phenotypic heterogeneity. In this work, we establish an auxotrophic complementation system for stable plasmid maintenance without antibiotic association, in Shewanella oneidensis, an attractive model exoelectrogen. A plasmid-transition strategy is designed to facilitate the rapid and efficient construction of the auxotrophic complementation system. Such a system not only enables the same intensive gene expression as the conventional antibiotic-associated plasmid system but also exhibits remarkably superior robustness and stability. With this system, the menaquinone pool of the extracellular respiratory chain is enhanced first independently and further synergized by engineering the Mtr conduit, leading to significantly promoted extracellular electron transfer (EET) outputs (up to 10.33-and 2.97-fold improvement in the maximum current density and the maximum output voltage) and heavy metal Cr(VI) reduction ability (5.15-fold improvement). This work provides a robust and stable platform to engineer exoelectrogens for environmental applications.

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“…In any case, the identification of the repB protein is interesting because it has been previously explored in S. xiamenensis BC01 and S. oneidensis MR-1 ( Zhou & Ng, 2016 ) as functional ori for stable plasmid replication in Shewanella . Furthermore, the combination of repB with other promoters such as placI favoured the expression of gfp and mtr genes by recombinant DNA technology in S. oneidiensis MR-1 ( Ng et al, 2018 ). In this way, specific plasmids could be constructed and incorporated into other Shewanella species to favour the expression of exogenous genes of interest ( Antonio-Hernández et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Pathogenic strains of Shewanella putrefaciens contain plasmids that are absent in the probiotic strain Pdp11

Domínguez-Maqueda

Pérez-Gómez

Grande-Pérez

et al. 2022

PeerJ

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Shewanella putrefaciens Pdp11 is a strain described as a probiotic for use in aquaculture. However, S. putrefaciens includes strains reported to be pathogenic or saprophytic to fish. Although the probiotic trait has been related to the presence of a group of genes in its genome, the existence of plasmids that could determine the probiotic or pathogenic character of this bacterium is unknown. In the present work, we searched for plasmids in several strains of S. putrefaciens that differ in their pathogenic and probiotic character. Under the different conditions tested, plasmids were only found in two of the five pathogenic strains, but not in the probiotic strain nor in the two saprophytic strains tested. Using a workflow integrating Sanger and Illumina reads, the complete consensus sequences of the plasmids were obtained. Plasmids differed in one ORF and encoded a putative replication initiator protein of the repB family, as well as proteins related to plasmid stability and a toxin-antitoxin system. Phylogenetic analysis showed some similarity to functional repB proteins of other Shewanella species. The implication of these plasmids in the probiotic or pathogenic nature of S. putrefaciens is discussed.

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Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1

Cited by 10 publications

References 41 publications

Mechanism study of photo-induced gold nanoparticles formation by Shewanella oneidensis MR-1

Mechanism study of photo-induced gold nanoparticles formation by Shewanella oneidensis MR-1

A designed plasmid‐transition strategy enables rapid construction of robust and versatile synthetic exoelectrogens for environmental applications

Pathogenic strains of Shewanella putrefaciens contain plasmids that are absent in the probiotic strain Pdp11

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