Effect of Moringaoleifera Lam. on haematological and histopathological parameters in sodium flouride induced toxicity in wistar rats

Abstract

The present investigation was undertaken to study the effect of Moringa oleifera Lam. on haematological and histopathological changes in the liver and kidney in wistar rats, induced with sodium fluoride toxicity and vitamin C. Thirty six female wistar rats were divided into six groups (T 1, T 2, T 3, T 4, T 5 and T 6 ), each comprising of six rats. T 1 served as negative control which received normal saline only. T 2 served as positive control, received sodium fluoride @ 20 mg/kg b.wt. only, T 3 received fluoride @ 20 mg/kg b.wt along with ascorbic acid @ 200 mg/kg b.wt and treatment was given to T 4 , T 5 and T 6 with different doses, viz., 250, 500 and 750 mg/kg b.wt of aqueous extract of M. oleifera, respectively along with sodium fluoride. The blood was collected after 28 days for serum haematological parameters study and then all the rats were sacrificed for histopathological studies. On total RBC count, though values were noted in increasing direction from graph but statistically analysed were found non significant. The haemoglobin, WBC and PCV were shown to be restored significantly on induced toxicity rats. Histopathological alterations in T 3 , T 4 , T 5 and T 6 were observed milder degree as compared to T 2 . The alterations in three (T 4 , T 5 and T 6 ) groups when compared to the fluoride administered group showed high degree of protective effect treatment on kidney, by using aqueous extracts shows the toxic changes.

Effect of Moringa olefiera on neurotoxicity induced by sodium fluoride in rats

“…A model for stimulating neurotoxicity in animals is the administration of sodium fluoride as described in [22]. In the present study, the administration of NaF caused an increase in total lipids, cholesterol, triglycerides, HDL, Causing a decrease in TAC, MDA, catalase, and SOD compared to the control group.…”

“…Rats were kept in stainless steel cages in hygienic conditions and fed for two weeks on a basal diet (BD) to adapt and supplied with adlibitum water. After a period of adaptation to the basaldiet, the rats were divided into 4 groups;Four diets were formulated first one contains basal diet as control (negative control),second one contain basal diet supplemented with 20 mg/kg b.wt sodium fluoride (positive control) [10], and the third one contains basal diet supplemented with 20 mg/kg b.wt + Moringa Oleifera 300 mg/kg,and fourth one contain basal diet supplemented with 20 mg/kg b.wt + Moringa Oleifera 500 mg/kg using stomach tube and fed to rats daily for 30days [10].…”

Safety profile of marbofloxacin following repeated intramuscular administration alone and piperine pretreated rats

“…Patel et al (2014) reported significant alteration in the values of neutrophils, lymphocytes, total erythrocytes and haemoglobin, but within normal clinical range following single intravenous administration of marbofloxacin at 2.0 mg/kg in sheep. Kamble et al (2017) observed no significant alterations in hematological parameters in sodium flouride induced toxicity in wistar rats.…”

Pharmacological Studies on Marbofloxacin on Diarrheic Calves

“…These data disagree with Modi et al, (2013) also detected no significant differences in RBCS and hemoglobin concentration after administration of marbofloxacin in sheep. Kamble et al, (2017) who observed no significant alterations in hematological parameters in sodium fluoride induced toxicity in Wister rat. Sadariya et al, (2010) reported that no significant changes in hematological paramters (Hemoglobin, RBC, WBC, MCV, MCH, MCHC, HCT and DLC) with marbofloxacin injection in rats.…”