Safety profile of marbofloxacin following repeated intramuscular administration alone and piperine pretreated rats

Abstract

A safety evaluation of marbofloxacin was carried out following repeated intramuscular (5 mg/kg of body weight for 5 days) administration alone and piperine pretreated (10 mg/kg, P.O. for 5 days) rats. The blood samples were collected into sterile K 3 EDTA and non-heparinized test tube on 0 day (before treatment) a nd 6 th day (after treatment) monitored by estimation of va rious haemato-biochemical parameters.The average values of hematological parameters, viz., HB, PCV, TLC, TEC, DLC, MCV, MCH and MCHC were estimated before and after drug administration. The average values of blood biochemical (ALT, AST, Total protein, Albumin, Globulin, LDH, Creatinine, BUN, Bilirubin and AKP) parameters were measured before and after drug administration. The values of HB, PCV, eosinophil and monocytes were found be significant (p<0.05) when compared to before treated values. No significant alterations in mean values of all serum biochemical parameters were observed after treatment compared to (0 day) before treatment. All the rats were found no clinical signs and others adverse reactions.It is concluded that marbofloxacin following repeated intramuscular alone and piperine pretreated rats did not show major significant alterations in the haemato-biochemical parameters. Marbofloxacin was found sa fe and well tolerated antibacterial agents to be used against bacterial infections.

Original article Evaluation of pathophysiological alterations in rats administered with marbofloxacin alone and with piperine pretreatment

“…did not evok significant differences in hematological and biochemical parameters between pre and post drug treatment period in sheep. The results of the present study are consistent with the same type of study performed in rats by Chauhan et al (2017), where the effect of piperine on repeated intramuscular administration of marbofloxacin (5.0 mg/kg -1 body weight repeated at 24 h interval for five days) in wistar rats did not alter (non-significantly) haemato-biochemical parameters.…”

Pharmacokinetics of ferulic acid following oral administration ethyl ferulate alone and in combination with piperine in rats

“…al. 2017 ab ) All pharmacokinetic parameter of ferulic did not differ significantly except volume of distribution (1.25 ± 0.12 vs 2.85 ± 0.57 L/kg), total body clearance (7.35 ± 0.57 vs 17.19 ± 1.59 L/h/kg) and mean residence time (0.38 ± 0.00 vs 0.40 ± 0.00 h), following Alteration in phamaracokinetic parameter of ferulic acid following co-administration of piperine with ethyl ferulate after single oral administration in rats might be due to interaction of piperine with enzymes that participate in drug metabolism, such as mixed function oxidases found in the liver and intestinal cells or may be due to inhibition of hepatic and non-hepatic drug metabolizing enzymes (Rondini et al, 2002;Poquet et al, 2008;Singh et al, 2009;Patel et al, 2019;Chauhan et al, 2017). Moreover, ferulic acid primarily metabolise in liver for decreasing the proportion of free ferulic and at the same time for increasing the proportion of conjugated ferulic acid to total ferulic acid in plasma (Zhoa et al, 2004).…”

“…Research report shows that absorption of ferulic acid after oral administration is quite rapid but bioavailability and mean residence time of it is low in rats (Rondini et al, 2002). So, in order to improve bioavailability, esterification of ferulic acid is one way (Biasutto et al, 2007) and to decrease in vivo metabolism, piperine can be favourable agent as bioenchaner (Singh et al, 2009;Chauhan et al, 2017;Patel et al, 2018). Ethyl ferulate is (ethyl-3hydroxy-4-methoxycinnamate) phenyl propanoid, alkyl ester derivative of ferulic acid.…”

Original article Pharmacokinetic profiles of marbofloxacin following single and repeated oral administration in broiler chickens

“…Important pharmacokinetic parameters of marbofloxacin like larger volume of distribution, optimum AUC and C max make it a potentially useful drug for the treatment of genital tract, respiratory tract, gastrointestinal tract, skin and soft tissue infections in domestic animals and birds (Brown, 1996;Ihrke et al, 1999). Along with higher efficacy, marbofloxacin is one of the safer floroquinolone compound as it did not produce any adverse reactions and significant alterations in biochemical and hematological parameters in rats after repeated intramuscular administration (Chauhan et al, 2017).…”