Effect of lentinan and mannan on phagocytosis of fluorescent latex microbeads by mouse peritoneal macrophages: A flow cytometric study

Ábel, György; Szöllősi, János; Chihara, Goro; Fachet, J

doi:10.1016/0192-0561(89)90146-x

Cited by 46 publications

(23 citation statements)

References 19 publications

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“…9) Polysaccharides from L. edodes and A. blazei have been expected to have anti-carcinogenic activities based on their antitumor eŠects, e.g., cytokine release and stimulation of phagocytosis. 1,3,4) Our results indicate that these polysaccharides possess the novel anti-carcinogenic function of suppressing CYP1A expression in vivo.…”

Section: Fig 2 Ešect Of Mushroom Polysaccharides On the Ex Vivomentioning

confidence: 80%

“…cytokine release 3) and the stimulation of phagocytosis. 4) Agaricus blazei has also been reported to include polysaccharides with immunomodulatory and antitumor activities. 5,6) Numerous studies have demonstrated that cytokines, including tumor necrosis factor (TNF)-a are involved in hepatic in‰ammation induced by such infectious stimuli as lipopolysaccharide (LPS).…”

mentioning

confidence: 99%

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Suppressive Effect of Polysaccharides from the Edible and Medicinal Mushrooms,Lentinus edodesandAgaricus blazei, on the Expression of Cytochrome P450s in Mice

Hashimoto

Nonaka

Minato³

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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Section: Fig 2 Ešect Of Mushroom Polysaccharides On the Ex Vivomentioning

confidence: 80%

mentioning

confidence: 99%

Suppressive Effect of Polysaccharides from the Edible and Medicinal Mushrooms,Lentinus edodesandAgaricus blazei, on the Expression of Cytochrome P450s in Mice

Hashimoto

Nonaka

Minato³

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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“…Various types of immunomodulators, glucans in particular, well are known to stimulate phagocytosis 19 . Therefore, the evaluation of this basic type of immune reaction is important to determine the eff ectiveness of any biologically active immunomodulator.…”

Section: Discussionmentioning

confidence: 99%

Glucan and resveratrol complex - possible synergistic effects on immune system

Větvička¹,

Volný²,

Saraswat-Ohri³

et al. 2007

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background: Recent data showing that glucan elicited defense responses in grapevine and induced protection via induction of resveratrol production led us to evaluate the possible synergetic eff ects of glucan and resveratrol complex on immune reactions.Methods: We measured phagocytosis using HEMA particles, expression of cell surface markers via fl ow cytometry, expression of cytokines using ELISA, recovery after fl uouracil-induced leucopenia and eff ects on gene expression via RT-PCR.Results: Our results showed that both glucan and resveratrol complex stimulated phagocytosis of blood leukocytes, caused increase in surface expression of CD + splenocytes and showed higher restoration of spleen recovery after experimentally induced leucopenia. In all these cases, strong synergetic eff ects were observed. When we measured the eff ects of these substances on expression level of NF-κB2, Cdc42 and Bcl-2 in breast cancer cells, upregulation of Cdc42 expression was evident only using both immunomodulators in combination.Conlusions: In conclusion, our data suggest signifi cant synergy in stimulation of immune reactions and support further studies of these natural immunomodulators.

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“…Lentinan is currently used clinically as an antitumor agent (18,19). It has been shown to stimulate natural killer cell activity (20 -22), macrophage/monocyte functions (secreting IL-1 and superoxide anion), phagocytosis, and cytotoxicity (23)(24)(25)(26)(27)(28). Lentinan was found to elevate the cytotoxic activity and TNF secretion of macrophages in vitro and in vivo (16,29).…”

mentioning

confidence: 99%

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

Pan

Zhang

et al. 2011

Journal of Biological Chemistry

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Lentinan, a cell wall ␤-glucan from the fruiting bodies of Lentinus edodes, is well known to be a biological defense modifier, but the signal transduction pathway(s) induced by Lentinan have not been elucidated. In this study, we extracted Lentinan (LNT-S) by ultrasonication from Lentinus edodes and report that, in murine RAW 264.7 macrophages, LNT-S glucan activated NF-B p65 and triggered its nuclear translocation as determined by Western blotting. Moreover, LNT-S enhanced NF-B-luciferase activity in the Dual-Luciferase gene system assay. Its upstream signaling molecules, MAPKs such as ERK1/2 and JNK1/2, were shown to be activated by assessing the level of phosphorylation in a time-and concentration-dependent manner, but its downstream proinflammatory enzyme, inducible NOS, was not observed. The data evaluated using a TNF-␣ ELISA kit and Griess reagent further demonstrated that no proinflammatory mediators such as TNF-␣ and NO were produced by LNT-S stimulation in RAW 264.7 cells. In contrast, LPS significantly induced inducible NOS expression and increased NO and TNF-␣ production, which are associated with activation of the NF-B p65/p50 heterodimer complex. It is possible that LNT-S did not activate NF-B p65/p50, and the activation of NF-B p65 was not sufficient to stimulate cytokine production. These data demonstrate that LNT-S glucan carries out its immunomodulating activity by activating MAPK signaling pathways without secretion of TNF-␣ and NO.

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Effect of lentinan and mannan on phagocytosis of fluorescent latex microbeads by mouse peritoneal macrophages: A flow cytometric study

Cited by 46 publications

References 19 publications

Suppressive Effect of Polysaccharides from the Edible and Medicinal Mushrooms,Lentinus edodesandAgaricus blazei, on the Expression of Cytochrome P450s in Mice

Suppressive Effect of Polysaccharides from the Edible and Medicinal Mushrooms,Lentinus edodesandAgaricus blazei, on the Expression of Cytochrome P450s in Mice

Glucan and resveratrol complex - possible synergistic effects on immune system

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

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