Temperature has a promising role in textile coloration process. This study intends to optimize the dyeing temperature for sustainable coloration of cotton fabric employing banana waste. Natural dye retrieved from banana floral stem by roller squeezer machine. To explore the effect of temperature, samples were dyed for 60℃, 70℃, 80℃, 90℃, 100℃ and 110℃ respectively by keeping constant time at 60 minutes. Effect of temperature variation on colorimetric appearance were expressed using CIE L * a * b * color space in terms of color coordinates , color strength (K/S), brightness index (BI) and degree of color levelness values. The dye fiber bonding stability was accessed via color fastness to wash, water, perspiration, rubbing and light. Except light fastness property almost color fastness value was 3-5 i.e. good to excellent. Best result of K/S, bright index % and color levelness value recorded 0.65, 50.58 and 0.069 respectively for 100 ℃. Excellent color fastness properties also appeared for 100℃. This reports prognosis an environmental begins approach for coloration of cotton fabric by deploying of banana plant waste.