Effect of incubation conditions, inhibitors, 2-mercaptoethanol and testosterone on RNA synthesis in ram spermatozoa

Salem, M. H.; Abdel-Kereim, M.A.; Selim, Ashraf; Pursel, V. G.

doi:10.1016/0093-691x(88)90181-1

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…Plasma membrane and acrosomal membrane PAA (PAA of the spermatozoa) as w.ell as sperm PA1 (anti-t-PA) could be affected directly and/or indirectly by testosterone only until late spermiogenesis; protein synthesis is known to occur until late spermiogenesis (Kramer & Ericson, 1982), with the exception of mitochondria1 protein which can be synthesized by mature spermatozoa (Salem et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Increased plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram after serum gonadotrophin (PMSG) administration. Correlation with the increased level of testosterone in the blood

et al. 2009

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After a single injection of serum gonadotrophin (PMSG) at the dose of 15 IU/kg, i.m. into rams testosterone in the plasma of blood showed a significant rise between 4th and 7th day post-injection. At the same time (4th-7th day) the plasminogen activator activity (PAA) in seminal plasma was found to be increased, but the plasminogen activator inhibition (PAI) expressed against t-PA (anti-t-PA) showed an increase between 32nd and 46th day. In spermatozoa a marked increase of PAA was revealed between 32nd and 46th day postinjection, while an increase of PA1 (anti-t-PA) was exhibited on the 74th day. Plasmin inhibition (PI) in seminal plasma and spermatozoa showed no change compared to controls. A positive correlation has been found between increased concentrations of testosterone and PAA or PA1 (anti-t-PA) in spermatozoa and seminal plasma. The induced increase of PAA in spermatozoa under the effect of testosterone might be of physiological importance, since PAA is localized to sperm membranes and might participate in the whole process of fertilization. not the acrosome reaction. J Reprod Fertil 65:185-194 Fritz IB, Karmally K (1983) Hormonal influences on formation of plasminogen activator by cultured testis tubule segments at defined stages of the cycle of the seminiferous epithelium. Can J Biochem Cell Biol 61:553-560 Garnier D-H, Cotta Y, Terqui M (1978) Androgen radioimmunoassay in the ram: results of direct plasma testosterone and dehydroepiandrosterone measurement and physiological evaluation. Ann Biol Anim Biochem Biophys 18 (2A):265-281 ANDROLOGIA 23, 273-278 (1991) 278 C. REKKAS ET AL. Huarte J, Belin D, Bosco D, Sappino A-P, Vassalli J-D (1987) Plasminogen activator and mouse spermatozoa: Urokinase synthesis in the male genital tract and binding of the enzyme to the sperm cell surface. J Cell Biol 104: 128 1-1289 Juhan-Vague I, Moerman B, De Cock F, Aillaud MF, Collen D (1984) Plasma levels of a specific inhibitor of tissue-type plasminogen activator (and urokinase) in normal and pathological conditions. Thromb Res 33:523-530 Kaminski JM, Bauer L, Mack SR, Anderson RA, Waller DP, Zaneveld LJD (1986) Synthesis and inhibition of human acrosin and trypsin and acute toxicity of aryl 4-guanidinobenzoates. J Med Chem 29:514-519 Kaminski JM, Mack SR, Zaneveld LJD (1988) A critical assessment of the interaction between active site inhibitors and proacrosin. Biol Reprod 38 (Suppl 1):342 Kirchheimer J, Binder BR (1983) Localization and immunological characterization of plasminogen activators in human prostate tissue. Haemostasis 13:358-363 Kokolis N (1990) Effect of orchidectomy on tissue plasminogen activator activity, plasminogen activator inhibition and plasmin inhibition. Haemostasis 20:233-240 Kramer JM, Ericson RP (1982) Analysis of stage-specific protein synthesis during spermatogenesis by two-dimensional gel electrophoresis. J Reprod Fertil 64:139-143 Lacroix M, Fritz IB (1982) The control of the synthesis and secretion of plasminogen activator by rat Sertoli cells in culture. Mol Cell Endocr 26:247-258 Lac...

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Section: Discussionmentioning

confidence: 99%

Increased plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram after serum gonadotrophin (PMSG) administration. Correlation with the increased level of testosterone in the blood

et al. 2009

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“…PI in seminal plasma, but not in spermatozoa, showed a positive and linear correlation with mean values and basal levels of blood testosterone in Friesland rams and with mean values of blood testosterone in Karagouniki rams. Spermatozoal PAA or PA1 could be affected directly and/or indirectly by testosterone only until late spermiogenesis since protein synthesis is known to occur until this stage (Kramer & Ericson, 1982) with the exception of mitochondrial protein which can be synthesized by mature spermatozoa (Salem et al, 1988). Plasminogen activators and plasminogen activator inhibitors in seminal plasma should predominantly be the result of their synthesis from epithelial cells of cauda epididymis, vas deferens, seminal vesicles, and prostate gland.…”

Section: Discussionmentioning

confidence: 99%

Breed and seasonal variation of plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram in correlation with testosterone in the blood

2009

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Summary. Plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI), against t‐PA (t‐PAI) or u‐PA (u‐PAI), in spermatozoa and seminal plasma as well as testosterone in the blood of Friesland, Chios, and Karagouniki rams all showed a seasonal variation with the highest values during the corresponding breeding season of the ewes (Autumn‐Winter). The seasonal variation of PAA and PAI in spermatozoa or seminal plasma as well as blood testosterone was different among the three breeds studied. Increased spermatozoal PAA was observed in November and May in Friesland rams, in October and November in Chios rams, and in October in Karagouniki rams. Spermatozoal t‐PAI was increased in December and June in Friesland rams, in November and December in Chios rams, and in November in Karagouniki rams. Spermatozoal u‐PAI was increased in December in Friesland rams, in October and December in Chios rams, and in November and December in Karagouniki rams. Plasminogen activator activity and PAI in seminal plasma also showed similar seasonal variations. Plasminogen activator activity and PAI in spermatozoa and seminal plasma showed a positive correlation with blood testosterone. The results of the present study support our previous findings on the possible role of spermatozoal PAA and PAI in the fertilizing ability of spermatozoa.

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“…In Experiment 1, BME and cysteine were added to the extender before freezing, in opposition to studies with frozen sperm of bulls (Bilodeau et al, 2001), boars (Yamaguchi andFunahashi, 2012) and rams (Salem et al, 1988;Bucak et al, 2008), in which antioxidants were added to extenders after thawing. The inclusion of antioxidants prior to freezing was aimed to minimize the production of superoxide radicals that commonly occur during all steps of sperm cryopreservation (Chatterjee and Gagnon, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Even though cystine cannot be metabolized by the cell, BME reacts with cystine producing a disulfide mixture that allows cysteine to become available (Ishii et al, 1981;Takahashi et al, 2002), releasing BME to react with other cystine molecules. Although BME stimulates synthesis of mRNA and of essential mitochondrial proteins required to produce energy for sperm motility (Salem et al, 1988), antioxidant effects of BME and cysteine on post-thawing ram sperm viability were not yet reported.…”

Section: Introductionmentioning

confidence: 99%

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé

Cardoso²,

Silva³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos ABSTRACTThe effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated.Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.

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Effect of incubation conditions, inhibitors, 2-mercaptoethanol and testosterone on RNA synthesis in ram spermatozoa

Cited by 9 publications

References 16 publications

Increased plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram after serum gonadotrophin (PMSG) administration. Correlation with the increased level of testosterone in the blood

Increased plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram after serum gonadotrophin (PMSG) administration. Correlation with the increased level of testosterone in the blood

Breed and seasonal variation of plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram in correlation with testosterone in the blood

Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

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