Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Pradieé, J.; Cardoso, Tainã Figueiredo; Silva, E. F.; Gonçalves, Alexandre Ortega; Gastal, G. D. A.; Rosa, Carlos Eduardo da; Mondadori, Rafael Gianella; Pegoraro, L. M. C.; Vieira, Arnaldo Diniz; Lucia, Thomaz

doi:10.1590/1678-4162-8479

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…The results suggest that the increase in apoptotic cells probably occurred at the expense of decreased motile spermatozoa in experimental samples. It is possible that spermatozoa are incapable of metabolizing some antioxidants as somatic cells do [35,43]. Similar to the present study, other authors have observed an increase in the Table 3.…”

Section: Potential Post-thawing Effect Of Vit C and Mesupporting

confidence: 88%

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Post thaw treatment of frozen buffalo semen with antioxidants vitamin C and 2-mercaptoethanol

Ivanova

Abadjieva

Gradinarska

et al. 2020

Biotechnology & Biotechnological Equipment

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The aim of this study was to evaluate the effect of ascorbic acid (Vitamin C) and 2-mercaptoethanol (ME) added to buffalo semen samples after thawing for 1 h and 2 h incubation. Ejaculates (n ¼ 20) from eight Bulgarian Murrah buffaloes were frozen in pellets and straws at a final sperm concentration of 80 Â 10 6 mL. We investigated the effect of supplementations on the motility, morphology of spermatozoa at 1 h and 2 h after thawing, sperm plasma membrane status and mitochondrial trans-membrane potential. The comparative CASA analysis of the motility and velocity parameters of spermatozoa showed significant differences in the progressive motility and in the VCL values in favour of control vs. samples with additives (p < 0.05). The molecular changes in PM phospholipid asymmetry were higher after thawing (p < 0.05). The semen treated with Vit C showed significant (p < 0.05) lower number of apoptotic spermatozoa (6CFþ/Ann V Cy3þ) in comparison with samples incubated with ME. The numbers of dead spermatozoa (6CF-/Ann V Cy3þ) in samples with ME were significantly higher than other groups. The percentage of spermatozoa with well preserved and functioning mitochondria in control semen was higher (p < 0.05) than that in the experimental group with ME. The results obtained revealed that addition of Vit C (5 mmol/L) and ME (50 mmol/L) has no positive effect on postthaw semen quality of buffalo sperm, because of the impaired integrity of PM and mitochondrial trans-membrane potential.

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Section: Potential Post-thawing Effect Of Vit C and Mesupporting

confidence: 88%

“…In the samples with ME there was a clearly visible swallowing of spermatozoa heads and acrosome region. In contrast, some studies report that ME did not influence the post thaw quality and oxidative activity of ram spermatozoa [35], but ME has been added in semen extender before the freezing process.…”

Section: Potential Post-thawing Effect Of Vit C and Mementioning

confidence: 91%

Post thaw treatment of frozen buffalo semen with antioxidants vitamin C and 2-mercaptoethanol

Ivanova

Abadjieva

Gradinarska

et al. 2020

Biotechnology & Biotechnological Equipment

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Use of Purslane (Portulaca oleracea) Extracts and Cysteine in Diluents to Improve the Cryopreserved Quality of Goat Sperm

Azimi

Farshad

Farzinpour

et al. 2023

Biopreservation and Biobanking

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Ram Semen Cryopreservation Using Egg Yolk or Egg Yolk-free Extenders: Preliminary Results

Ptáček

Stádníková

Savvulidi

et al. 2019

Scientia Agriculturae Bohemica

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Kinematic parameters of thawed ram semen frozen under field conditions were analysed with the use of two commercial (egg yolk vs egg yolk free) semen extenders in different year-seasonal periods. The semen was collected from Suffolk (n = 2) and Charollais (n = 1) rams kept in private breeding farm on 3 test days (pre-mating, at mating, post mating) during year 2016. Two commercial semen extenders (egg yolk-based BullXcell® or egg yolk-free AndroMed®) were used for semen processing. Processed semen was frozen in 0.25 ml plastic cryostraws using the custom-made portable freezing box for ram semen cryopreservation under field conditions. Thawed semen characteristics were evaluated using computer-assisted semen analysis (CASA) system immediately after thawing and after 2 h of heat incubation (±38°C). Significantly higher total motility (+12.3%), straight line velocity (+5.6 μm s−1), and average-path velocity (+6.9 μm s−1) were detected for the semen processed and cryopreserved using egg yolk-based semen extender. Year-seasonal variation and introducing the ram to service had shown to have a significant effect on the cryopreserved ram spermatozoa. These preliminary results confirmed the feasibility of ram semen processing and cryopreservation under field conditions.

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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

Cited by 3 publications

References 26 publications

Post thaw treatment of frozen buffalo semen with antioxidants vitamin C and 2-mercaptoethanol

Post thaw treatment of frozen buffalo semen with antioxidants vitamin C and 2-mercaptoethanol

Use of Purslane (Portulaca oleracea) Extracts and Cysteine in Diluents to Improve the Cryopreserved Quality of Goat Sperm

Ram Semen Cryopreservation Using Egg Yolk or Egg Yolk-free Extenders: Preliminary Results

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