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Iron is an important mineral for animal development and function. The present study was carried out to evaluate the effect of FeSO 4 replacement with cysteine-coated Fe 3 O 4 nanoparticles in quail. We hypothesised that the iron nanoparticles could be used as a dietary supplement. The quails were randomly allotted to five dietary treatments with four replicate pens and 10 birds per pen. The treatments consisted of: negative control without any iron supplementation, positive control with 120 mg/kg of FeSO 4 and three experimental groups that contained 1.2, 12 and 120 mg/kg of L-cysteine coated iron oxide nanoparticles, organic iron, in their diet, respectively. The least weight gain was recorded in the negative control group. Similar weight gain, FCR, haematological and biochemical parameter results between the experimental and positive control groups show that organic iron successfully performed the biological role of iron in the quail. We concluded that 1.2 mg/kg cysteine-coated Fe 3 O 4 nanoparticles are required and sufficient for quails' optimal maintenance and growth. The results confirm that cysteine-coated Fe 3 O 4 nanoparticles can be used as an iron source in the quail diet.

Silver nanoparticles could prove to be a valuable alternative raw material for antibiotics and disinfectants as it is relatively free of adverse effects. Nanosilver is now been put to practical use in commonly used items, such as, clothes, electric home appliances, and electronic industry, but has not been widely applied in the medical or pharmacological fields. This study was designed to investigate the effects of nanosilver on egg quality traits in laying Japanese quail in completely randomized design with four treatments and six repetitions at 0, 4, 8 and 12 ppm of silver nanoparticle levels. Eggs collected daily and egg parameters, including egg weight, length, width; yolk weight and eggshell thickness were examined. The effect of the different silver nanoparticle levels was determined using the General Linear Model of SAS procedure, whilst differences between the groups were determined using least significant difference test. Results indicated that silver nanoparticles at all levels caused significantly reduce of yolk weight and hen-day egg production for each week than the control treatment (P \ 0.05), whilst silver nanoparticle had no significant effect on egg weight, egg length and width and eggshell thickness.

Contents Rooster fertility peaks between 30 and 40 weeks of age and declines rapidly from 50 weeks of age. This is linked to a reduction in the number of spermatozoa in the ejaculate due to high density of spermatids in the seminiferous tubules of low‐fertility roosters. In this study, we assessed the effects over spermatogenesis of both letrozole, an aromatase inhibitor, and a commercial herbal extract designed for improving fertility composed by Tribulus, Cinnamomum, Zingiber and Sativus. Forty‐two‐week‐old Ross 308 roosters (n = 24) were distributed into four groups: control, letrozole (0.03 mg/kg), herbal extract (0.04 ml/kg) and letrozole+herbal extract. After 14 weeks of daily oral supplementation, their testes and epididymides were weighed, fixed and sectioned for assessment of spermatogenesis and quantification of sperm cells inside the lumen. Differences in seminiferous tubules measurements and density of sperm cells were tested using R software (version 3.0.1). Although body weight was not affected by the treatment, testes from animals treated with the combination of letrozole and herbal extracts were heavier than those from control animals. Animals treated with either letrozole or herbal extract, or their combination, showed a significant higher number of sperm cells inside the seminiferous tubules and epididymis than control animals (p < .05). These data suggest that the use of letrozole and the herbal extract could improve sperm cell production in ageing roosters. Future studies are needed to disclose the causal mechanisms involved and its effect on fertility and ejaculate features.