Effect of immobile isolated enzymes from rumen liquid by using alginate matrices on the bay leaf extraction

Abstract

BSTRACTAbstract.This research aims to develop the enzymatically of bay leaves phytochemical extraction process. The novelty and the main innovations of this research is the development of extraction process by using enzymatic extractor and isolate the enzymes from rumen liquid to shift the equilibrium phase, increase the extraction rate and increase the extraction yield. The activity of rumen liquid enzyme was represented by the activity of cellulase and protease. The analyze of total flavonoid content was performed by using UV-Vis Spectrofometry. The activity of immobilized enzyme of cellulase (0.080.00 U/ml) was lower than the un-immobilized one (0.230.00 U/ml). However, there was no difference activity of the immobilized (0.750.00 U/ml) and un-immobilized (0.760.01 U/ml) of protease. The model of mass transfer of un-immobilized enzyme can be fitted on the experimental data, however the model of mass transfer of immobilized enzyme did not match with the experimental data. The mass transfer coefficient of enzymatic extraction flavonoids bay leaf without immobilization was 0.17167 s -1 which greater than the reported value of obtained KLa from extraction by using electric heating.

Design of Stirred Tank Reactor for Bioetanol Production From Banana Rod Using Cellulase Enzymes Cow's Liquid Rumen

“…Testing the total glucose content begins with the absorbance test of the bioethanol solution using the Genesys 20 Spectrophotometer in 540 nm. Cellulase enzyme that isolated from rumen liquid under cold condition shows a high activity of enzyme (Paramita et al 2015) that use in hydrolisis process. From the standard curve is obtained it can be obtained average of glucose content is around 1,494 ± 0,007.…”

Determination of Influential Factors during Enzymatic Extraction of Ginger Oil using Immobile Isolated Cow Rumen Enzymes

“…Considering that zingiberene is a thermolabile compound, the proposed process should be able to extract ginger oil quickly in order to minimize the use of energy, and with controllable temperatures to prevent thermal degradation. Enzymatic extraction techniques are believed to have many advantages over conventional extraction, such as high yields, high selectivity, and being environmentally friendly (Avilla et al, 2005;Panouile et al, 2007;Ptichkina et al, 2008;Paramita et al, 2015). Although there are several patents for enzymatic extraction processes (US Patents No.…”