Determination of Influential Factors during Enzymatic Extraction of Ginger Oil using Immobile Isolated Cow Rumen Enzymes

Abstract

With respect to its multiple uses, such as in cosmetics, foods, aromatherapy and the pharmaceutical industry, ginger oil has a high value in the world market. The ginger oil obtained from conventional extraction usually has low zingiberene content, possibly due to thermal degradation. To overcome this problem, an alternative ginger oil production process by enzymatic extraction using cow rumen enzymes is investigated. The aim of the research is to obtain the optimum conditions for zingiberene-rich ginger oil extraction by using immobile isolated cow rumen enzyme. The experiments were conducted under varying temperatures (40-60 o C), enzyme-substrate ratios (0.05-0.2) and extraction times (1-5 days). The microwave assisted distillation was conducted for 90 minute to separate the ginger oil from its mixture. The zingiberene content in the oil was measured by GC analysis. The most influential factor in the enzymatic extraction of ginger oil was determined by experimental design 23. Analysis of the results shows that for the extraction with a rumen ratio of 1:5 at 60 o C, the most influential factor was the extraction time, in this case 5 days, and ginger oil was obtained with zingiberene contents of 21.56% and 26.28% at pH 5 and pH 4 respectively. Prolonging the extraction time to 6 days with pH 5 caused a decrease in zingiberene content to 20.76%.

Ginger waste as a potential feedstock for a zero-waste ginger biorefinery: a review

“…The optimal time (5 days) allowed cow rumen enzymes to hydrolyze polysaccharides in the cell wall, resulting in more isolated ginger oil (26.28%). 16…”

“…The optimal time (5 days) allowed cow rumen enzymes to hydrolyze polysaccharides in the cell wall, resulting in more isolated ginger oil (26.28%). 16 In another work, Das et al compared and contrasted the effect of UAE with respect to MAE of ginger rhizome peel on the total phenolic content (TPC, see Table 3) and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl, DPPH). The MAE methodology resulted in higher TPC and antioxidant activity compared to UAE and cold maceration of ginger rhizome peel and rhizome.…”

Desain Eksperimental Faktorial Untuk Penentuan Faktor Paling Berpengaruh Pada Proses Pulping Organosolv Berbahan Baku Limbah Daun Nanas

“…Banyak penelitian menggunakan metode Desain Eskperimental dalam menentukan factor paling berpengaruh pada berbagai proses termasuk dalam proses ekstraksi zingiberene jahe [16][17]. Dalam penelitian ini, metode desain eksperimental dirancang dan dianalisis untuk membuktikan hipotesis.…”

Immobilization of Cholesterol Oxidase in Chitosan Magnetite Material for Biosensor Application

“…The process parameters of temperature, enzyme substrate concentration, and extraction time have the greatest effects on the enzymatic reaction. In addition, the reaction slows down unless enzyme is continuously added (Handayani et al, 2018). However, a study of enzymes used as biocatalysts to produce biodiesel has shown that immobilization of the lipase enzyme by an adsorption-crosslinking method stabilized the enzyme and kept it soluble in the reaction.…”