Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ

Dewi, Nurdiana; Syaify, Ahmad; Wahyudi, Ivan Arie

doi:10.20473/j.djmkg.v46.i3.p135-139

Cited by 4 publications

(8 citation statements)

References 14 publications

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“…The induction of hyperglycemia by injection of a single dose STZ 40 mg/kgBW in this research might cause increased blood glucose levels until above 120 mg/dl of pregnant rat, in the other hand also seen the hyperglycemia pregnant rat had increasing of feed consumption (polyphagia), water consumption (polydipsia), and urine production which characterized by easily wet in husks (polyuria) [3,4].…”

Section: Discussionmentioning

confidence: 72%

“…This result supported by Dewi (2014) who said that the tooth germ width of rats offspring in hyperglycemia group was smaller than control group, the researcher considered that matter due to increase of blood glucose levels which correlated with occurrence of malnutrition. As known that increasing blood glucose levels of pregnancy rat in this research associated with pancreatic β-cells damage by the presence of DNA methylation reducing the amount of NAD + and increasing free radical production through the action of cell toxicity [3]. Free radicals would cause DNA damage and reduced the ability of pancreas to produce insulin and then cause disruption metabolism of carbohydrates, fats, and proteins that are useful as a source of nutrition [4].…”

Section: Discussionmentioning

confidence: 99%

“…Enamel contains inorganic ingredients up to 96% and dentin 70%, and around 37% of these inorganic ingredients is calcium. Nutrition also influences the speed of tooth collagen matrix and noncollagen tissue synthesis that the place for calcium salts deposition [3,14,16].…”

Section: Discussionmentioning

confidence: 99%

“…formation inhibited than postnatal rat offspring born from pregnant rat who did not suffer from hyperglycemia [3].…”

Section: Romanian Journal Of Diabetes Nutrition and Metabolic Diseases mentioning

confidence: 93%

“…This process was causing enamel hypoplasia, delay in tooth eruption, and decrease in length of alveolar bone. Protein synthesis was known to be very importance on formation matrix and hard tissue calcification process on teeth [3,4].…”

Section: Romanian Journal Of Diabetes Nutrition and Metabolic Diseases mentioning

confidence: 99%

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The Effect of Thymoquinone Toward Tooth Disorder Prevention in Postnatal Rat offspring Born from Hyperglycemia Pregnant Rats

Rachmawati

Hikmah

Indriana

et al. 2019

Romanian Journal of Diabetes Nutrition and Metabolic Diseases

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Background and aims. Hyperglycemia in pregnancy was caused by reduced insulin production that led to tooth germ growth disorder. Thymoquinone could increase insulin production through pancreatic β-cells regeneration. The purpose of this study was to determine the effect of thymoquinone to prevent tooth disorder in rat offspring born from hyperglycemia pregnant rat. Material and method. Wistar rat offspring used in this research taken from pregnant rat induced hyperglycemia with streptozotocin 40 mg/kgBW divided into four groups. Rat offspring observation had been done on 3rd, 5th, and 7th day postnatal. The histological image of first maxillary molar tooth germ stained with Haematoxilin Eosin and Mallory’s Trichrome. The parameters of rat offspring were body weight, blood glucose levels, tooth growth stages, tooth dimension width, and tooth germ enamel matrix. Results. Thymoquinone group had the widest tooth germ dimension compare to other groups. All rat offspring tooth germs were at the appositioncalcification stage in matrix enamel (pre-enamel). The analysis showed that no statistical differences between thymoquinone group and metformin group (p>0.05). Conclusions. Thymoquinone has same function with metformin to prevent tooth disorder in rat offspring born from pregnant rat induced hyperglycemia.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…formation inhibited than postnatal rat offspring born from pregnant rat who did not suffer from hyperglycemia [3].…”

Section: Romanian Journal Of Diabetes Nutrition and Metabolic Diseases mentioning

confidence: 93%

Section: Romanian Journal Of Diabetes Nutrition and Metabolic Diseases mentioning

confidence: 99%

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The Effect of Thymoquinone Toward Tooth Disorder Prevention in Postnatal Rat offspring Born from Hyperglycemia Pregnant Rats

Rachmawati

Hikmah

Indriana

et al. 2019

Romanian Journal of Diabetes Nutrition and Metabolic Diseases

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The enamel thickness of gestational diabetes mellitus rat offspring

Dewi

Syaify

Wahyudi

2021

J Dentomaxillofac Sci

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Objective: Gestational Diabetes Mellitus is diagnosed when Diabetes Mellitus begins or is first detected during pregnancy. Enamel hypoplasia is an abnormality in tooth enamel that was common found in children born to diabetic mothers. The aim of this study was to determine the molar enamel thickness of rat offsprings born to diabetic rat. Material and Methods: This research used sixteen rat offsprings born to 8 diabetic rats who had been injected with STZ dose of 40 mg / kg and 16 rat offsprings born to 8 control rats. Rat offsprings were decapitated on day 5 after birth and taken right mandibular molar tooth germ.Tooth germ then embedded in paraffin block. Haematoxylin eosin staining performed for histological analysis. Enamel thickness measured on the images using ImageJ Software at 5 field of view. Results: The enamel thickness on the DM group (333.02 ± 135.81 µm) were lower than control group (415.39 ± 146.17 µm). Mann Whitney test results showed a significant difference between the DM and control group. Conclusion: It was concluded that enamel thickness decrease in gestational diabetes mellitus rat offspring.

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Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus)

Christiono,

Pradopo,

Sudiana

et al. 2023

Dent. J.

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Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.

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Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ

Cited by 4 publications

References 14 publications

The Effect of Thymoquinone Toward Tooth Disorder Prevention in Postnatal Rat offspring Born from Hyperglycemia Pregnant Rats

The Effect of Thymoquinone Toward Tooth Disorder Prevention in Postnatal Rat offspring Born from Hyperglycemia Pregnant Rats

The enamel thickness of gestational diabetes mellitus rat offspring

Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus)

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