Effect of fluoride ion on proliferation of vero cell line cells: Growth acceleration by sodium fluoride.

Oguro, Akira; Koizumi, Nobuo; Horii, Kin-ichi

doi:10.5834/jdh.31.453

Cited by 13 publications

(9 citation statements)

References 17 publications

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“…2). Oguro et al reported a growth inhibitory effect of fluoride on established cell lines at concentrations of about 20 ppm (depending on cell strains), with a complete inhibition at 40-60 ppm concentrations (24). Our observations are in agreement with these data, suggesting that the sensitivity of the human epithelial cells to fluoride is similar to that of other cell lines (e.g., HeLa cells, kidney fibroblasts, gingival fibroblasts) (18,26).…”

Section: Measurement Of Fluoride Concentrationssupporting

confidence: 91%

Low concentration fluoride stimulates cell motility of epithelial cells in vitro

et al. 2009

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Oral mucosal tissue can serve as a long-term fluoride reservoir following topical application and retain a small amount of fluoride in oral environment for prevention of dental caries. The aim of this study was to determine the effect of low level sodium fluoride (NaF) on the proliferation and migration of epithelial cells in vitro. Human primary gingival epithelial cells and human epidermal HaCaT keratinocytes were used. Cultured epithelial cells, treated with various concentrations of NaF ranging from 5 μM to 500 μM, were investigated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, wound healing assay, invasion assay and quantitative real-time PCR. MTS assay revealed that fluoride added to human gingival epithelial cells elevated cell proliferation at a concentration of 5 μM or more. The wound healing assay and invasion assay confirmed this observation. Quantitative real-time PCR revealed that low concentration of NaF up-regulated fibronectin mRNA expression in fluoride-treated cells compared with controls. These results suggest that a low concentration of NaF is able to induce cell proliferation, migration, and matrix production in epithelial cells. Our results provide new information on epithelial cell adhesion and may thus aid in the understanding of periodontal physiology.

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Section: Measurement Of Fluoride Concentrationssupporting

confidence: 91%

Low concentration fluoride stimulates cell motility of epithelial cells in vitro

et al. 2009

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“…tal pulp cells to fluoride is similar to that of other cell lines (e.g., Hela cells, kidney fibroblasts, gingival fibroblasts) [26,27]. Our data showed that lower F concentrations (10 ppm) stimulate cell growth, as previously described [25]. …”

Section: Cell Growthsupporting

confidence: 75%

“…Oguro et al [25] reported a growth inhibitory effect of F on established cell lines at concentrations of about 20 ppm (depending on cell strains), with a complete inhibition at 40--60 ppm concentrations. Our observations are in agreement with these data, suggesting that the sensitivity of the human den- Each column represents the mean of 12 samples.…”

Section: Cell Growthmentioning

confidence: 99%

Selective inhibition of collagen synthesis by fluoride in human pulp fibroblastsIn Vitro

1993

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Human dental pulp cells were cultured in fluoridated mediums (0, 1, 10, 25 ppm) in order to study the biological effect of the ion regarding the cellular metabolism: cell growth, alkaline phosphatase (ALP) activity, and protein synthesis. The results indicated a decrease of the cell growth at 25 ppm and a dose-dependent decrease of the ALP activity. Type I collagen immunoperoxidase staining, radioimmunoassay quantitation, and analysis of type I and III collagens mRNA levels showed an inhibition of collagen production and gene expression. In contrast, fibronectin production and gene expression were not affected by fluoride. The treatment did not influence the qualitative pattern of the different mRNA species. Of the three collagen chains, the alpha 1(I) was the most affected. These data suggest that fluoride does not exert a general depletive effect on human dental pulp cells but rather a selective inhibition on collagen production.

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“…NaF was the first and still most recommended fluoride compound for fluoridation of drinking water (Ribeiro et al 2006). Fluoride has shown considerable variation in respect of cytotoxicity against different cultured cells (Hongslo et al 2004;Oguro et al 1983). Other manifestation of fluoride biological activity includes fluoride toxic effects -skeletal fluorosis and damage to kidney liver, parathyzoid glands and brain (Wang et al 2004;Shan et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

et al. 2008

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The number of biomaterials used in biomedical applications has rapidly increased in the past two decades. Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetically prepared composite that in its structure contains the same molecular concentration of OH − groups and F − ions. The aim of this experimental investigation was to use the embryonal mouse fibroblast cell line NIH-3T3 for comparative study of basal cytotoxicity of fluoridated biomaterials FHA and FA discs. Hydroxyapatite (HA) disc, high-density polyethylene as negative control and polyvinyl chloride (PVC) containing organotin stabilizer as positive control were used as standard biomaterials. The appropriateness of the use of NIH-3T3 cells and their sensitivity for tested biomaterials were evaluated on the basis of five cytotoxic end points: cell proliferation, cell morphology, lactate dehydrogenase (LDH) released, protein and DNA cell content. The basal cytotoxicity of FHA, FA and HA discs was measured by direct contact method. FHA composite, FA and HA demonstrated in cell line NIH-3T3 nearly similar basal cytotoxicity increasing with the time of treatment. After 72 h of biomaterials treatment, about 25% inhibition of cell number, unchanged morphology of dividing cells, 6.31-0.16% increase of released LDH, about 10% inhibition of cell protein content and about 20% inhibition of DNA content was found. On the other hand, from the growth rates it resulted that NIH-3T3 cells, affected by tested biomaterials, divided about 20% slowlier than the control (untreated cells). Using the linear regression analysis we found out that deviations in measurements of cytotoxicity by four methods were as follows: less than 10% for cell number, protein and DNA content methods and 12.4% for released LDH method. Based on a good correlation of the cytotoxicity of biomaterials obtained from all end points we could conclude that fibroblast NIH-3T3 cell line was appropriate for measuring the basal cytoxicity of tested biomaterials.

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Effect of fluoride ion on proliferation of vero cell line cells: Growth acceleration by sodium fluoride.

Abstract: Abstract:The effect of fluoride ion

Cited by 13 publications

References 17 publications

Low concentration fluoride stimulates cell motility of epithelial cells in vitro

Low concentration fluoride stimulates cell motility of epithelial cells in vitro

Selective inhibition of collagen synthesis by fluoride in human pulp fibroblastsIn Vitro

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

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