Low concentration fluoride stimulates cell motility of epithelial cells in vitro

Arakawa, Yuki; Bhawal, Ujjal K.; Ikoma, Takeharu; Kimoto, Kazunari; Kuroha, Kazumi; Kubota, T.; Hamada, Naoki; Kubota, Eiro; Arakawa, Hirohisa

doi:10.2220/biomedres.30.271

Cited by 27 publications

(17 citation statements)

References 28 publications

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“…We previously reported that low level NaF is able to induce cell motility, migration, invasion and matrix production in epithelial cells 23) . In this study, we identify the proteins involved in fluoride signaling and determine the role of these molecules in epithelial mesenchymal interactions in oral mucosa.…”

Section: Discussionmentioning

confidence: 95%

Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa

Bhawal

Hamada

et al. 2013

J. Hard Tissue Biology.

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Oral mucosa consists of the superficial epithelium and the underlying lamina propria, and it functions as a barrier against exogenous substances. In development, interactions of stem/progenitor cells of the epithelium and mesenchyme are crucial to the morphogenesis of oral mucosa. Our previous work in low level fluorideinduced cell motility of epithelial cells has yielded important clues for periodontal physiology. This study focuses on a working concept of low level fluoride to provide a conducive oral environment for pivotal epithelialmesenchymal interactions. Cultured human primary gingival epithelial cells treated with 50 μM NaF were investigated by DNA microarray. Quantitative real-time PCR and an in vivo experimental rat skin wound model were employed to confirm the findings obtained with the microarray analysis. Microarray data revealed that low level fluoride-treated human gingival epithelial cells elevated various biological processes. The key proliferation markers, FGF2 and FGF7, and mesenchymal marker, Twist1, expression was up-regulated and quantitative real-time PCR confirmed this observation. Our in vivo study revealed that low concentration of NaF increased FGF2, FGF7, and Twist1 protein expression in fluoride-treated skin wound tissues compared with controls. These results provide new information on low level fluoride-induced epithelial-mesenchymal interactions and may thus aid in the understanding of oral mucosa development.

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Section: Discussionmentioning

confidence: 95%

Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa

Bhawal

Hamada

et al. 2013

J. Hard Tissue Biology.

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“…The fluoride concentrations in our experiments were chosen according to a previous study that resulted in measurable effects on cultured cells. 19 Fluoride can inhibit the function of isolated osteoclasts at 0.5 to 1.0 mmol·L −1 in vitro . 20 Fluoridated drinking water markedly inhibited the bone resorption induced by hydrocortisone 21 and showed a lower histologic incidence of alveolar bone resorption after the injection of parathormone.…”

Section: Discussionmentioning

confidence: 97%

Micromolar sodium fluoride mediates anti-osteoclastogenesis in Porphyromonas gingivalis-induced alveolar bone loss

et al. 2015

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Osteoclasts are bone-specific multinucleated cells generated by the differentiation of monocyte/macrophage lineage precursors. Regulation of osteoclast differentiation is considered an effective therapeutic approach to the treatment of bone-lytic diseases. Periodontitis is an inflammatory disease characterized by extensive bone resorption. In this study, we investigated the effects of sodium fluoride (NaF) on osteoclastogenesis induced by Porphyromonas gingivalis, an important colonizer of the oral cavity that has been implicated in periodontitis. NaF strongly inhibited the P. gingivalis-induced alveolar bone loss. That effect was accompanied by decreased levels of cathepsin K, interleukin (IL)-1β, matrix metalloproteinase 9 (MMP9), and tartrate-resistant acid phosphatase, which were up-regulated during P. gingivalis-induced osteoclastogenesis. Consistent with the in vivo anti-osteoclastogenic effect, NaF inhibited osteoclast formation caused by the differentiation factor RANKL (receptor activator of nuclear factor κB ligand) and macrophage colony-stimulating factor (M-CSF). The RANKL-stimulated induction of the transcription factor nuclear factor of activated T cells (NFAT) c1 was also abrogated by NaF. Taken together, our data demonstrate that NaF inhibits RANKL-induced osteoclastogenesis by reducing the induction of NFATc1, ultimately leading to the suppressed expression of cathepsin K and MMP9. The in vivo effect of NaF on the inhibition of P. gingivalis-induced osteoclastogenesis strengthens the potential usefulness of NaF for treating periodontal diseases.

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“…Each well was washed twice with PBS to remove debris and to smooth the edge of the scratch and then replaced with the serum‐free Huaier aqueous extract (4 mg/mL). After that, the migration of cells in the “scratch” was photographed at the matching reference points with phase‐contrast microscope for the first image, and the following images were taken at intervals of 12 h. ( 20,21 ) The images were analyzed quantitatively by ImageJ; the distances between the two edges of the scratch were measured at the reference points and analyzed statistically.…”

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Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis

Zhang¹,

Kong²,

et al. 2010

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Aqueous extract of Trametes robiniophila murr (Huaier) has been commonly used in China for cancer complementary therapy in recent years; however, the mechanisms of its anticancer effects are largely unknown. In the present study, we aim to investigate its inhibitory effect on both MCF-7 and MDA-MB-231 cells, and explore the possible mechanisms of its anticancer effect. Cell viability and motility were measured by MTT and invasive assays, migration and scratch assays in vitro, respectively. The distribution of cell cycle, PI-Annexin-V staining and Rhodamine 123 assay were analyzed by flow cytometry, and western blot were used to test the apoptotic pathways. We found that Huaier extract could strongly inhibit cell viability of MCF-7 and MDA-MB-231 cells in a time-and dose-dependent manner; however, MDA-MB-231 cells showed more susceptibility to the treatment. Furthermore, cell invasiveness and migration were also suppressed with exposure to Huaier extract. We also indicated that Huaier could induce G0/ G1 cell-cycle arrest, p53 accumulation and activation selectively in MCF-7 cells. Inspiringly, the PI-Annexin-V staining assay and western blot analysis confirmed cell apoptosis executed by caspase-3. Decreased mitochondrial membrane potential by Rhodamine 123 assay and down-regulation of Bcl-2 and up-regulation of BCL2-associated X protein (BAX) indicated that Huaier induced apoptosis through the mitochondrial pathway. Caspase activation during Huaier-induced apoptosis was confirmed by pan-caspase inhibitor, Z-VAD-fmk. As expected, the inhibitor decreased Huaier-induced apoptosis in both cell lines. Based on our findings, Huaier can induce cell apoptosis in both ER-positive and ER-negative breast cancer cell lines and is an effective complementary agent for breast cancer treatment. (Cancer Sci 2010; 101: 2375-2383 W orldwide, it is estimated that more than 1 million women are diagnosed with breast cancer every year, and it accounts for approximately 410 000 deaths per year.( 1) Breast cancer is already the leading cause of cancer in southeast Asian women, and is second only to gastric cancer in east Asian women.(2) In some areas of China, the incidence of breast cancer is increasing by 5% per year, greater than that of the worldwide rate.(3) However, compared with other carcinomas, breast cancer has a better prognosis and over 5 million successful survivors comprise nearly 23% of the total cancer survivors in the USA. Although the 5-year survival is estimated at 98% and 94% for stage 1 and 2 localized disease, (4) respectively, the therapeutic options for advanced-stage breast cancers are still fairly limited.(5)

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Low concentration fluoride stimulates cell motility of epithelial cells in vitro

Cited by 27 publications

References 28 publications

Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa

Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa

Micromolar sodium fluoride mediates anti-osteoclastogenesis in Porphyromonas gingivalis-induced alveolar bone loss

Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis

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