Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

Abstract

The number of biomaterials used in biomedical applications has rapidly increased in the past two decades. Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetically prepared composite that in its structure contains the same molecular concentration of OH − groups and F − ions. The aim of this experimental investigation was to use the embryonal mouse fibroblast cell line NIH-3T3 for comparative study of basal cytotoxicity of fluoridated biomaterials FHA and FA discs. Hydroxyapatite (HA) disc, high-density polyethylene as negative control and polyvinyl chloride (PVC) containing organotin stabilizer as positive control were used as standard biomaterials. The appropriateness of the use of NIH-3T3 cells and their sensitivity for tested biomaterials were evaluated on the basis of five cytotoxic end points: cell proliferation, cell morphology, lactate dehydrogenase (LDH) released, protein and DNA cell content. The basal cytotoxicity of FHA, FA and HA discs was measured by direct contact method. FHA composite, FA and HA demonstrated in cell line NIH-3T3 nearly similar basal cytotoxicity increasing with the time of treatment. After 72 h of biomaterials treatment, about 25% inhibition of cell number, unchanged morphology of dividing cells, 6.31-0.16% increase of released LDH, about 10% inhibition of cell protein content and about 20% inhibition of DNA content was found. On the other hand, from the growth rates it resulted that NIH-3T3 cells, affected by tested biomaterials, divided about 20% slowlier than the control (untreated cells). Using the linear regression analysis we found out that deviations in measurements of cytotoxicity by four methods were as follows: less than 10% for cell number, protein and DNA content methods and 12.4% for released LDH method. Based on a good correlation of the cytotoxicity of biomaterials obtained from all end points we could conclude that fibroblast NIH-3T3 cell line was appropriate for measuring the basal cytoxicity of tested biomaterials.

Surface physical chemistry properties in coated bacterial cellulose membranes with calcium phosphate

“…It is interesting the range of the concentration because the material can be cytotoxic at some concentration and in other it cannot, besides, it is important to create a similar body fluid on the biomaterial contact. Therefore, these materials did not significantly affect the cell viability, being non-cytotoxic, corroborating with recent literature results [21,22].…”

Effects of Surface Charge of Hyperbranched Polymers on Cytotoxicity, Dynamic Cellular Uptake and Localization, Hemotoxicity, and Pharmacokinetics in Mice

“…Theiszova and co-workers have shown that the 3T3 fibroblast is appropriate for measuring the cytotoxicity of biomaterials. 43 Moreover, because it is a commonly utilized cell line, it provides a good system for comparison of nanomaterial safety as developed by different research groups. As key cellular components of the innate immune system, macrophages readily phagocytose foreign materials, including most nanoparticles, 44,45 and thus the in vitro toxicity response of RAW 264.7 macrophages is thought to provide preliminary insight on potential immune recognition of nanomaterials.…”

“…As the toxicity response to nanomaterials has previously proved to be cell-type dependent, , the present study compared the cytotoxicity of hyperbranched polymers in two different cell lines, 3T3 fibroblasts and RAW264.7 macrophages. Theiszova and co-workers have shown that the 3T3 fibroblast is appropriate for measuring the cytotoxicity of biomaterials . Moreover, because it is a commonly utilized cell line, it provides a good system for comparison of nanomaterial safety as developed by different research groups.…”

In vitro/In vivo assessment and mechanisms of toxicity of bioceramic materials and its wear particulates

“…Similar G0/G1 arrest and inhibition in the cell proliferation were found in FA treated NIH-3T3 cells, revealing the harmful genotoxic effect of uoridated biomaterial eluates. 143,144 Heil et al 17 reported a thorough study on the mutagenesis/ carcinogenesis of dental material extract, using three fast and reliable test systems, namely the bacterial umuC-test, the eukaryotic DNA synthesis inhibition test and an in vivo alkaline lter elution technique. The bacterial umuC test, using the Salmonella typhimurium strain, is as sensitive as an Ames test, with a umuC operon capable of being induced by genotoxic agents.…”