Selective inhibition of collagen synthesis by fluoride in human pulp fibroblastsIn Vitro

Abstract

Human dental pulp cells were cultured in fluoridated mediums (0, 1, 10, 25 ppm) in order to study the biological effect of the ion regarding the cellular metabolism: cell growth, alkaline phosphatase (ALP) activity, and protein synthesis. The results indicated a decrease of the cell growth at 25 ppm and a dose-dependent decrease of the ALP activity. Type I collagen immunoperoxidase staining, radioimmunoassay quantitation, and analysis of type I and III collagens mRNA levels showed an inhibition of collagen production and gene expression. In contrast, fibronectin production and gene expression were not affected by fluoride. The treatment did not influence the qualitative pattern of the different mRNA species. Of the three collagen chains, the alpha 1(I) was the most affected. These data suggest that fluoride does not exert a general depletive effect on human dental pulp cells but rather a selective inhibition on collagen production.

Fluoride at non-toxic dose affects odontoblast gene expression in vitro

“…Concerning dentin, fluoride was shown to change collagen synthesis (Veron et al, 1993). Proteoglycans were altered in their glycosaminoglycan side chains (Waddington and Langley, 1998), resulting in a hampered extraction from fluoride-treated tooth slices (Waddington et al, 2004).…”

Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

“…[8] It is now an established fact that fluoride ingestion over a period of time can affect the structure and function of cells, tissues, organs, and systems resulting in a variety of clinical manifestations. The various hard tissue effects of fluorosis are hypomineralization of enamel, dentin,[910] hypercementosis,[11] recession of alveolar crest,[11] root resorption,[12] and hypermineralization,[13] while the soft tissue changes include inhibition of Type I collagen synthesis,[14] degree of cross linking,[14] fibroblast growth inhibition,[15] lethal effects on fibroblasts,[161718] and morphologic changes. [15]…”

Comparative assessment of fluorosed and nonfluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after scaling and root planning and ethylenediaminetetraacetic acid root biomodification

“…The two reasons that lead to this study are, the in vitro toxic effects of fluoride on morphology and functions of fibroblasts [8][9][10][11] and difference in therapeutic response of fluorosed and nonfluorosed roots to nonsurgical periodontal treatment. [22,23] Following periodontal therapy, the initial step in periodontal wound healing such as fibroblast attachment between fluorosed and nonfluorosed roots requires to be evaluated.…”

“…Fluorosis is one of the major public health problems affecting the rural Indian population who are dependent on ground alveolar crest, [4] root resorption, [5] hypermineralization, [6] while the soft tissue changes include gingival recession, [4] initial mineralization and globular mineralized deposits in the periodontal ligament area, [6] inhibition of Type I collagen synthesis, [7] degree of cross linking, [7] fibroblast growth inhibition, [8] lethal effects on fibroblasts [9][10][11] and morphologic changes. [8] The effect of fluorosis on dental caries is welldocumented.…”