Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Abstract

Background:Fluorosis causes mineralization changes in the tooth and can lead to morphologic alterations of fibroblasts. To understand the effect of fluorosis on periodontal healing, the initial step during healing, such as fibroblast attachment to the root surface, needs to be evaluated. Hence, the objective of the present study was to study the attachment of fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments.Materials and Methods:A total of 56 fluorosed and nonfluorosed, periodontally healthy and diseased tooth roots were obtained and allotted to eight groups: Fluorosed and nonfluorosed healthy controls (FH and NFH, respectively), fluorosed and nonfluorosed diseased controls (FD and NFD, respectively), fluorosed and nonfluorosed roots treated with scaling and root planing (FD + S and NFD + S, respectively), and similar groups treated with scaling and root planing and 24% ethylenediaminetetraacetic acid (EDTA) gel application for 2 min (FD + SE and NFD + SE, respectively). After the respective treatment, the root fragments were incubated in the human periodontal ligament fibroblast cells obtained and cultured from freshly extracted healthy human fluorosed premolar tooth root.Results:In the nonfluorosed roots category, greater attachment was found in the untreated nonfluorosed diseased (P = 0.036) and SRP-treated nonfluorosed diseased groups (P = 0.008) as compared to the nonfluorosed healthy group. While in the fluorosed roots category, no significant difference was observed in FL-FA (P > 0.05) within the group. However, no attachment was observed in EDTA-treated fluorosed root fragments. When fluorosed groups were compared to nonfluorosed groups, no significant changes were noted between the groups.Conclusion:SRP proves to be a standard requirement for fibroblast attachment to occur both in fluorosed and nonfluorosed roots. Although there was no significant difference in attachment between SRP and SRP + EDTA among fluorosed roots, EDTA does not seem to be a promising agent for root biomodification in fluorosed roots in a given concentration and time of treatment.

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

“…The results of the current study showed that the cell viability of the fibroblasts attached to roots treated with scaling only was the highest compared with all other groups, and that scaled teeth treated with EDTA or CHX did not enhance cell attachment. This is in agreement with other studies which demonstrated that SRP is the standard requirement for attachment of fibroblasts and that root treatment with EDTA did not enhance cell viability or attachment over SRP alone when measured using either stereomicroscope or scanning electron microscope ( Girotra and Vandana, 2014 , Lima et al, 2016 ). Additionally, other researchers stated that the exposure of fibroblasts to ≥0.02% CHX reduced the survival rate of the cells in vitro ( Liu et al, 2018 ).…”

Fluorosis and periodontium: A report of our institutional studies

“…Neha G and Vandana [12] conducted a study to evaluate the attachment of fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments and concluded that SRP proves to be a standard requirement for fibroblast attachment to occur both in fluorosed and nonfluorosed roots. Although there was no significant difference in attachment between SRP and SRP + EDTA among fluorosed roots, EDTA does not seem to be a promising agent for root bio-modification in fluorosed roots in a given concentration and time of treatment.…”

“…The institutional studies presented above apart from being preliminary were all self-funded and carried out with the single motive of exploring the effect of fluorosis on the periodontium. The age group of the population in these studies was between 15 and 74 years for epidemiological survey [2] and a constant 18-25 years (periodontally healthy teeth extracted due to orthodontic reasons) in in vitro studies [3][4][5][6][7][8][9][10][11][12][13][14][15] that allows for better comparisons of study results within the same study population. In an epidemiological survey, a strong association of occurrence of periodontal disease in high-fluoride areas was found.…”

“…[11] • Attachment of fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments after SRP and EDTA root bio-modification. [12] • A study to compare the attachment of nonfluorosed and fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments. [13] Root surface changes following LASER irradiation in flurorosed teeth…”

The Use of Subepithelial Connective Tissue Graft Technique to the Treatment of Class I Miller Recession in Lower Tooth with Abrasion: Case Report

“…The purpose of the EDTA conditioning, in this study, was to demineralize the root and removal of smear layer produced due to instrumentation to facilitate fibroblast adhesion and locomotion by collagen exposure [19,20]. The in vitro and in vivo effects of EDTA root biomodification are not consistent evident [20,21].…”