Effect of diets containing different types of sardine waste (Sardinella sp.) protein hydrolysate on the performance and intestinal morphometry of silver catfish juveniles (Rhamdia quelen)

Abstract

Sardinella sp. protein hydrolyzate in the feed of Rhandia quelen 957 Lat. Am. J. Aquat. Res., 44(5): 957-966, 2016 "Pathways for sustainable industrial fisheries in southeastern and southern Brazil" P.M. Arana, P.R. Pezzuto, A.O. Ávila-da-Silva, D. Queirolo, J.A.A. Perez & C.A. Arfelli (eds.). Research ArticleEffect of diets containing different types of sardine waste (Sardinella sp.) protein hydrolysate on the performance and intestinal morphometry of silver catfish juveniles (Rhamdia quelen) ABSTRACT. In this study two fractions of muscle hydrolysate were tested, soluble and insoluble (FSM and FIM), they were assessed individually and combined with each other (FSM+FIM). Also, two fractions of hydrolyzed viscera: soluble of natural and industrialized viscera (FSVN and FSVI) were tested on the performance and on the intestinal morphology of juvenile catfish (Rhamdia quelen). The experimental design was completely randomized with five treatments and five replicates. Juveniles were kept in aquariums with density of eight fish per tank and were cultured for 56 days. The results were analyzed using parametric variance analysis (ANOVA) and subjected to the Duncan test (5% significance level). The best results on final weight, weight gain, feed conversion rate, and protein efficiency ratio were obtained with the diet containing FSM+FIM, and the diet containing the FSVI. The latter was also the most consumed by the animals. The diet containing FIM was the one that provided the worst consumption results. The worst feed conversion rate was obtained for the diet containing FSM. Survival and body composition did not differ between treatments. The separation of the soluble and insoluble fractions is not necessary or even recommended when the goal is to use the muscle hydrolysate as the ingredient in feed for silver catfish juveniles. The best performance results obtained were with the combination of soluble and insoluble fractions of muscle hydrolysate, and with the industrial viscera soluble hydrolysate. The degree of hydrolysis has a direct effect on feed consumption. Keywords: Rhamdia quelen, enzymatic hydrolysis, fish waste, animal feed, aquaculture. . El diseño experimental fue completamente aleatorio con cinco tratamientos y cinco réplicas. Los juveniles se mantuvieron en acuarios con una densidad de ocho peces por estanque y se cultivaron durante 56 días. Los resultados se analizaron mediante análisis de varianza paramétrico (ANOVA) y se sometieron a la prueba de Duncan (nivel de significación 5%). Los mejores resultados de peso final, ganancia de peso, conversión alimenticia, y la tasa de eficiencia proteica, se obtuvieron con la dieta que contenía FSM+FIM, y la dieta que contenía el FSVI. Esta última fue también la más consumida por los animales. La dieta que contiene el FIM fue la que proporcionó los peores resultados de consumo. Se obtuvo el peor índice de conversión alimentaria con la dieta que contiene el FSM. La supervivencia y la composición corporal no difirieron entre tratamientos. La separación de las ...

Fish meal replaced by hydrolysed soybean meal in diets increases growth and improves the antioxidant defense system of silver catfish ( Rhamdia quelen )

“…The DH obtained in the HSM was 14.1%, which is low when compared to previous studies (Fabregat et al, ; Venturin et al, ; Wosniak et al, ). The peptide profile analysis shows that 39.8% of the HSM had a molecular weight of less than 10 kDa.…”

“…In this study, we chose for an inclusion level of 20% of HSM. The addition of high levels of hydrolysates in diets to silver catfish can lead to problems with nutrient leaching and metabolic losses (Wosniak et al, , Venturini et al, ). Silver catfish is an omnivorous fish with a nutritional requirement of carnivorous fish (Meyer & Fracalossi, ).…”

“…We emphasize that this is the first work with soybean hydrolyzed to silver catfish. Other works with the species have used hydrolysates from animal origin (Fabregat et al, ; Venturini et al, ; Wosniak et al, ). Thus, the partial substitution of fishmeal with HSM in diets for juvenile silver catfish was evaluated for growth, protein metabolism and antioxidant activity.…”

Tilapia protein hydrolyzate improves growth performance, protein absorption and antioxidant status in Silver catfish ( Rhamdia quelen )

“…The degree of hydrolysis (DH) of the TPH was 20.1%, which is similar to the 10%–20% observed for the Sardinella sp. muscle hydrolyzate (Fabregat et al, ; Venturin et al, ; Wosniak et al, ). The DH indicates the percentage of broken peptide bonds, which affect the physicochemical and functional properties of the hydrolyzate (Wasswa, Tang, Gu, & Yuan, ).…”

Pathways for sustainable industrial fisheries in southeastern and southern Brazil

“…As part of IGEPESCA work plan, Amorim et al (2016) and Wosniak et al (2016) extracted protein hydrolysates from viscera, heads and carcasses of the most important fishing resources of Brazil, the Brazilian sardine and the whitemouth croaker. These were not only shown to be of acceptable quality but in fact, Wosniak et al (2016) demonstrated that they could be successfully used as food in catfish farms of southern Brazil. While preliminary in nature, these studies represent innovative solutions to the fishing industry crisis of southeastern and southern Brazil, where fish waste can reach sizeable proportions.…”