Effect of dietary resveratrol in ameliorating aflatoxin B1‐induced changes in broiler birds

Abstract

Consumption of aflatoxin B1 (AFB1) contaminated feed by poultry affects the health of broiler birds causing severe economic losses. The use of phytochemicals is a safe, effective, alternative and practical approach to combat the toxic effect of AF in broilers. Resveratrol, a polyphenol derived from red grapes, berries and peanuts, exerts anti-inflammatory, antioxidant and immunomodulatory effects. Our study was aimed at evaluating the possible protective effects of resveratrol against the adverse effects of AFB1 in broiler birds. A feeding trial of 42 days of duration was undertaken in a completely randomized design with five dietary treatments: G1-AFB1(1.0 ppm); G2-CTR (basal diet alone); G3-AFB1(1.0 ppm)+Resv 0.5%; G4-AFB1(1.0 ppm)+Resv 1%; and G5-Resv 1%. Gain in body weight (BWG) and feed intake (FI) was observed to be highest (p < 0.05) in the AFB1 birds followed by the control group. Feed conversion ratio was lowest in G2-CTR birds and failed to record any significant variation (p > 0.05) between groups as well as within groups. Birds fed resveratrol at both 0.5% and 1.0% levels in combination with AFB1 as well as alone along with basal diet had lower BWG and FI between the fourth and fifth week and also at the fifth week (p < 0.05). No variation (p > 0.05) was obtained in the FCR of AFB1 and resveratrol group of broiler birds. AFB1 feeding significantly increased the activities of aspartate-(AST) and alanine-(ALT) amino transferase, superoxide dismutase (SOD) and catalase (CAT) activities (p < 0.05) but lowered glucose, cholesterol and triglyceride levels in serum. Supplementation of resveratrol helped in increasing the activities of the oxidative enzymes and in improving the plasma total antioxidant capacity (TAOC) and total protein (TP) significantly (p < 0.05) and protein values. The livers of AFB1 group showed degeneration of hepatocytes, bile duct hyperplasia and microgranuloma formation. In resveratrol supplemented birds, the severity and degree of the liver lesions was far less. Apoptotic proteins failed to show any variation in expression between AFB1, control and resveratrol group of birds. The inclusion of resveratrol in broiler diets enhanced antioxidant status of birds indicating the protective effect of resveratrol against AFB1-induced toxicity. So, we advice use of resveratrol as a feed additive to control aflatoxicosis in poultry farms.

Effects of aflatoxin B1 alone or co-administered with Mycotox NG on performance and humoral immunity of turkey broilers.

“…Mycosorbents are able to bind aflatoxin molecules in the gastrointestinal tract and thus, to reduce their absorption by domestic fowl. The results agree with data from other studies with mycosorbents as clinoptiolite (Oguz et al, 2003), hydrated calcium aluminosilicate (HSCAS), sodium bentonite, montmorillonite (Ologhobo et al, 2015), essential oils (Saei et al, 2013), antioxidants (resveratrol) (Sridhar et al, 2015) and probiotics (Zuo et al, 2013).…”

Broiler Tavuklarda Aflatoksin ile Kontamine Diyette Ticari Toksin Bağlayıcı, Doğal Probiyotik Türleri, Maya Hücre Duvarı ve Aluminosilikatın GOT2 ve CYP450 1A5 Gen Ekspresyonları İle Karaciğer Enzimlerinin Serum Konsantrasyonları Üzerine Etkisi

“…The result of GOT2 gene expression in PC. group was consistent with the findings of Sridhar et al [39] which showed the increasing of AST serum levels by consuming aflatoxin contaminated diets. Also L., B., Y. and BLY.…”

Potential of plant polyphenols to combat oxidative stress and inflammatory processes in farm animals

“…In the study of Sridhar et al. (), supplementation of resveratrol (at a level of 1% of diet) improved the antioxidative status of serum in broilers, both without and with further treatment with aflatoxin B1. In that study, dietary treatment with resveratrol, however, impaired body weight gains and feed conversion ratio.…”