Effect of cyclosporine A on the structure of adult albino rat testis and the role of lycopene supplementation

Alazouny, Zeinab M.; Mohamed, Eman M.; Ahmed, Gehan A.-R.

doi:10.1097/01.ehx.0000446585.19443.d3

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…The present finding were in accordance with the results recorded by Alazouny et al (2014) where Examination of the seminiferous tubules showed disruption of their architecture and some tubules were empty with no germinal epithelium and others contained sloughed germ cells. Blood vessels congestion was also present.…”

Section: Discussionsupporting

confidence: 93%

“…Additionally, these free radicals enhance lysosomal enzymes release in the cytoplasm and oxidation of cellular protein leading to their disruption. All these effects lead to appearance of vacuoles due to degeneration of germ cells and increased spaces between Sertoli cells (Alazouny et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…It is obtained from Cylindrocarpon lucidum and Tolypocladium inflatum fungi. It was firstly used as an antibiotic but it also has some fungicidal and antiparasitic effects (Fahr, 1993& Alazouny et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

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The ameliorative effect of N-acetylecysteine on cyclosporine induced testicular toxicity in male albino rats

Abdelkader

Hilal

Torky

et al. 2021

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Introduction: Cyclosporine A (CsA) is considered one of the potent drugs that are used extensively in organ transplant and oncology patients. It is also used in autoimmune diseases. Unfortunately, its use is accompanied with several hazards; one of these is testicular toxicity. Nacetylecysteine (NAC) is an antioxidant found to reduce CsA toxicity. Aim of the work: The study aims to determine the toxic effect of exposure to cyclosporine on the testis and to investigate the protective role of NAC. Methods: The study conducted on 50 adult male albino rats for 4 weeks divided into 5 groups, group A the negative control group, group B the olive oil group (0.5 ml/d orally), group C the cyclosporine group (25mg/kg/d orally), group D the NAC group (600mg/kg/d orally) and group E the cyclosporine+NAC group. At the end of the study the evaluation was achieved by biochemical analysis and histopathology. Results: Cyclosporine significantly affects the testis morphologically and functionally. The morphological changes are in the form of degenerative changes in the tubules with dislocation of germ cells into the lumen and irregular outlines, Congestion of blood capillaries in the interstitial tissue, and functionally the cyclosporine cause significant decrease in serum testosterone level. While co-administration of NAC with cyclosporine attenuate these effects. Conclusion: Cyclosporine causes testicular injury through oxidative stress and NAC as an antioxidant attenuates but not fully protect against cyclosporine induced testicular toxicity.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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The ameliorative effect of N-acetylecysteine on cyclosporine induced testicular toxicity in male albino rats

Abdelkader

Hilal

Torky

et al. 2021

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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“…Group II (lycopene group): This group included 5 rats received lycopene at a dose of 10 mg/kg body weight dissolved in corn oil as a vehicle orally by gastric tube daily for 4 weeks [21] .…”

Section: Experimental Designmentioning

confidence: 99%

Histological and Immunohistochemical Study of Titanium Dioxide Nanoparticle Effect on the Rat Renal Cortex and the Possible Protective Role of Lycopene

Salem

Altayeb

El-Mahalaway

2017

Egyptian Journal of Histology

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Introduction: Titanium dioxide nanoparticles (TNPs) have a wide range of applications in industry, medicine and environmental technology. Nowadays, TNPs have raised researcher's concerns on their toxicity. Lycopene is a dietary carotenoid having a potent antioxidant effect. Aim: To evaluate the effect of TNPs on the structure of renal cortex of rats and to assess the possible protective effect of lycopene against TNPs nephrotoxicity. Material and methods: Forty five adult male rats were divided into four groups. Group I (the control group) included 20 rats, which were divided equally into 4 subgroups. Subgroup IA was the negative control, Subgroup IB given one ml corn oil by gastric tube, Subgroup IC given one ml distilled water intraperitoneally (i.p) and Subgroup ID given one ml corn oil by gastric tube and one ml distilled water (i.p). Group II included 5 rats received a daily dose of 10 mg/kg body weight (BW) of lycopene by gastric tube for 4 weeks. Group III included 10 rats injected with 150 mg/kg BW (i.p.) of TNPs daily for 4 weeks. Group IV included 10 rats received both TNPs and lycopene at the same aforementioned doses for 4 weeks. Kidney specimens were prepared and sections were stained with hematoxylin and eosin and Masson's trichrome. Immunohistochemical detection of desmin, anti-proliferating cell nuclear antigen (PCNA) and caspase-3 was done. Results: Our results revealed that group II had similar findings nearly as group I. Group III showed congested dilated glomerular capillaries. The convoluted tubules showed exfoliation of some lining epithelial cells and degenerative changes. There were interstitial haemorrhage, mononuclear cell infiltration and increase in collagen fibers around degenerated convoluted tubules and glomeruli. A significant increase in desmin-expression in the glomerular cells was observed. Besides, there were significant increases of PCNA positive nuclear and caspase-3 cytoplasmic reactions in renal tubular cells. Group IV revealed amelioration of these changes. Conclusion: Lycopene protected rat's renal cortex against TNPs nephrotoxicity.

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“…There are many immunosuppressive agents used in transplant medicine one of them is cyclosporine A (CsA) which is a neutral agent that is derived from Cylindrocarpon lucidum Booth and Tolypocladium inflatum Gams fungi. Cyclosporine is used as an antibiotic, antifungal and antiparasitic agent (Fahr, 1993& Alazouny et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

Experimental study of renal toxicity of cyclosporine and the ameliorative effect of N-acetylecysteine in albino rat

Abdelkader

Hilal

Torky

et al. 2021

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Introduction: Cyclosporine A (CsA) is considered powerful immunosuppressive drug which has improved the quality of life and survival rate of transplant patients and also used in autoimmune diseases. However, its use is limited by many side effects mainly nephrotoxicity. NAC is an antioxidant found to reduce CsA toxicity. Aim of the work: The study aims to determine the effect of exposure to cyclosporine on the kidney and to investigate the protective role of NAC. Methods: the study conducted on 50 adult male albino rats for 4 weeks, divided into 5 groups, group A the negative control group, group B the olive oil group (0.5 ml/d orally), group C the cyclosporine group (25mg/kg/d orally), group D the NAC group (600mg/kg/d orally) and group E the cyclosporine+NAC group. At the end of the study the evaluation was done by biochemical analysis and histopathology. Results: cyclosporine significantly affects the kidney by morphological changes in the form of dilatation of urinary space with congestion and lobulation of glomerullar capillaries in the renal corpusle. Proximal convoluted tubules showed degeneration of their cells with irregularity and destruction of brush border. Degeneration of distal convoluted tubules with exfoliation of some cells inside the lumen and the peritubular capillaries were congested and extravasated. Also cyclosporine affects the kidney by increasing serum urea and creatinine levels, while coadministration of NAC with cyclosporine attenuate its effects. Conclusion: cyclosporine causes renal injury through oxidative stress and NAC as an antioxidant attenuates but not fully protect against cyclosporine induced injuries.

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Effect of cyclosporine A on the structure of adult albino rat testis and the role of lycopene supplementation

Cited by 9 publications

References 31 publications

The ameliorative effect of N-acetylecysteine on cyclosporine induced testicular toxicity in male albino rats

The ameliorative effect of N-acetylecysteine on cyclosporine induced testicular toxicity in male albino rats

Histological and Immunohistochemical Study of Titanium Dioxide Nanoparticle Effect on the Rat Renal Cortex and the Possible Protective Role of Lycopene

Experimental study of renal toxicity of cyclosporine and the ameliorative effect of N-acetylecysteine in albino rat

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