Histological and Immunohistochemical Study of Titanium Dioxide Nanoparticle Effect on the Rat Renal Cortex and the Possible Protective Role of Lycopene

Abstract

Introduction: Titanium dioxide nanoparticles (TNPs) have a wide range of applications in industry, medicine and environmental technology. Nowadays, TNPs have raised researcher's concerns on their toxicity. Lycopene is a dietary carotenoid having a potent antioxidant effect. Aim: To evaluate the effect of TNPs on the structure of renal cortex of rats and to assess the possible protective effect of lycopene against TNPs nephrotoxicity. Material and methods: Forty five adult male rats were divided into four groups. Group I (the control group) included 20 rats, which were divided equally into 4 subgroups. Subgroup IA was the negative control, Subgroup IB given one ml corn oil by gastric tube, Subgroup IC given one ml distilled water intraperitoneally (i.p) and Subgroup ID given one ml corn oil by gastric tube and one ml distilled water (i.p). Group II included 5 rats received a daily dose of 10 mg/kg body weight (BW) of lycopene by gastric tube for 4 weeks. Group III included 10 rats injected with 150 mg/kg BW (i.p.) of TNPs daily for 4 weeks. Group IV included 10 rats received both TNPs and lycopene at the same aforementioned doses for 4 weeks. Kidney specimens were prepared and sections were stained with hematoxylin and eosin and Masson's trichrome. Immunohistochemical detection of desmin, anti-proliferating cell nuclear antigen (PCNA) and caspase-3 was done. Results: Our results revealed that group II had similar findings nearly as group I. Group III showed congested dilated glomerular capillaries. The convoluted tubules showed exfoliation of some lining epithelial cells and degenerative changes. There were interstitial haemorrhage, mononuclear cell infiltration and increase in collagen fibers around degenerated convoluted tubules and glomeruli. A significant increase in desmin-expression in the glomerular cells was observed. Besides, there were significant increases of PCNA positive nuclear and caspase-3 cytoplasmic reactions in renal tubular cells. Group IV revealed amelioration of these changes. Conclusion: Lycopene protected rat's renal cortex against TNPs nephrotoxicity.

Histopathological and genetic study on the protective role of -Carotene on testicular tissue of adult male albino rats treated with titanium dioxide nanoparticles

“…As regards Masson's trichrome, staining sections from control group revealed normally distributed collagen fibers in the capsule and around blood vessels, results that were in agreement with those described by Abd Ellatief et al 2015; but in treated group revealed marked increase of collagen fibers especially in the capsule and around blood vessels; this result is in accordance with Salem et al (2017) and Altindag et al (2007) who proposed that the increased collagen fibers are due to the decrease in collagen metabolism that may be related with oxidative stress. In protective group, there was normal distribution of collagen fibers especially in testicular capsule and around blood vessels, a result that is in agreement with Gopal et al (2015).…”

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

“…Regarding renal tubules, some were degenerated with numerous tubular dilation and most of their lumens contained homogenous acidophilic cellular debris. Some tubular cells appeared with dark stained pyknotic nuclei while others showed cytoplasmic degeneration or vacuolations in consistent with Altayeb et al, (2017) [38] who revealed changes as; flattening of the epithelial lining of some tubules, exfoliation and pyknosis of some tubular cells with apparent luminal dilatation and intratubular cell debris.…”

“…Fartkhooni et al, (2016) [37] in their study also reported swelling and dilatation of Bowman's capsule. Also, Altayeb et al, (2017) [38] documented several renal histopathological changes associated with dilated congested glomerular capillaries. Regarding renal tubules, some were degenerated with numerous tubular dilation and most of their lumens contained homogenous acidophilic cellular debris.…”

RETRACTED ARTICLE: The teratogenic effect of pregabalin on heart, liver and kidney in rats: a light microscopic, electron microscopic and immunohistochemical study

“…Fibroblasts increase their numbers and secrete huge amounts of extracellular matrix. Finally, prolonged injury to the renal parenchyma and renal failure take place [12,42].…”