Effect of chitosan nanoparticle, QMix, and EDTA on TotalFill BC sealers’ dentinal tubule penetration: a confocal laser scanning microscopy study

Aydın, Zeliha Uğur; Özyürek, Taha; Keskin, Büşra; Baran, Talat

doi:10.1007/s10266-018-0359-0

Cited by 33 publications

(47 citation statements)

References 36 publications

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“…In the present study, the percentage of the sealer penetration in the 4 mm sections was greater than that in the 2 mm sections for all except the MDA group. The difference between apical and coronal sections has also been noted in previous studies [5,7,38]. One possible explanation for this difference is the decreased density and diameter of dentinal tubules in the apical root dentin, with some areas completely devoid of tubules [29,39].…”

supporting

confidence: 69%

Effectiveness of Various Final Irrigation Techniques on Sealer Penetration in Curved Roots: A Confocal Laser Scanning Microscopy Study

Yılmaz

Yalçın

Helvacıoğlu-Yiğit

2020

BioMed Research International

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Objective. To compare the efficacy of various techniques used for final irrigation on sealer penetration in the apical one-third of curved root canals. Material and Methods. Sixty-five freshly extracted maxillary first molar teeth with mesiobuccal roots having more than 20° of root curvature were used. The root canals were instrumented and randomly divided into four experimental groups and one control group. In the 4 experimental groups, 3 ml of 17% EDTA followed by 3 ml of 5.25% NaOCl was delivered with the use of the following protocols: Group 1: manual dynamic activation (MDA), Group 2: sonic irrigation (SI), Group 3: passive ultrasonic irrigation (PUI), and Group 4: conventional needle irrigation (CI). All teeth were obturated with gutta-percha and AH Plus sealer labeled with fluorescent dye. Transverse sections at 2 mm and 4 mm distance from the root apex were examined with the aid of confocal laser scanning microscopy. Total percentage (%) and maximum depth (μm) of sealer penetration were measured. Results. All the experimental groups exhibited significantly higher penetration rates than the control group at both sections (p<0.05). However, no significant differences were found in the penetration depth and percentage among the four experimental groups evaluated at both sections (p>0.05). Conclusion. PUI, SI, and MDA did not significantly improve sealer penetration in the apical portion of curved root canals when compared to conventional needle irrigation.

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supporting

confidence: 69%

Effectiveness of Various Final Irrigation Techniques on Sealer Penetration in Curved Roots: A Confocal Laser Scanning Microscopy Study

Yılmaz

Yalçın

Helvacıoğlu-Yiğit

2020

BioMed Research International

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“…In contrast, 17% EDTA has no detergent in its composition and presents high surface tension and low permeability, which limits its chelating effect 33 . Interestingly, other studies showed that 17% EDTA and QMiX promoted similar sealer penetration 10,30,31 . www.nature.com/scientificreports www.nature.com/scientificreports/ Overall, the efficacy of the final irrigation protocols for debris/smear layer removal and sealer penetration decreased from the coronal to the apical thirds.…”

Section: Groupsmentioning

confidence: 90%

“…The effect of final irrigation with QMiX on tubular dentin sealer penetration was compared with 17% EDTA in previous studies 10,30,31 . However, to our knowledge, this is the first study to report the effects of PUI with QMiX on tubular dentin sealer penetration.…”

Section: Groupsmentioning

confidence: 99%

The effect of 17% EDTA and QMiX ultrasonic activation on smear layer removal and sealer penetration: ex vivo study

Matos

Silva

Paranhos

et al. 2020

Sci Rep

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This study aimed to compare the effect of conventional irrigation (CI) and passive ultrasonic irrigation (PUI) with 17% EDTA and QMiX on the maximum depth and percentage of sealer penetration into the dentinal tubules by confocal laser scanning microscopy (CLSM) and to describe the cleaning of root canal walls by scanning electron microscopy (SEM). Eighty single-rooted human mandibular premolars were instrumented and randomly assigned to four groups (n = 20): EDTA + CI, QMiX + CI, EDTA + PUI, and QMiX + PUI. Ten samples from each group were examined by SEM (2,000×) and the remaining 40 roots were filled with a single gutta-percha cone and AH Plus sealer mixed with 0.1% rhodamine B for analysis by CLSM (10×). Images were assessed at distances of 2 mm (apical), 5 mm (middle), and 8 mm (coronal) from the apex with the Leica Application Suite V4.10 software. The EDTA + PUI and QMiX + PUI protocols presented higher rates of debris/smear layer removal in the apical and middle thirds. The PUI was superior to CI in the maximum depth of sealer penetration at the middle third. The QMiX + PUI group had a higher percentage of sealer penetration at the apical third. The PUI and QMiX protocol improved debris/smear layer removal and tubular dentin sealer penetration.

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“…In dentistry, CH has shown antibiofilm (8) and chelating effects (7)(8)(9)(10). Its ability to remove the smear layer, similar to that of 17% EDTA and of calcium hydroxide intracanal medicament, has been confirmed by previous studies (6,7,(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 65%

“…Acetic acid (AA) can remove dentin calcium ions, favoring smear layer removal, but it can also reach the inorganic structure of dentin, causing subsequent erosion and changes in dentin microhardness (6). In order to obtain a chelating effect without harming the dentin content or periapical tissues, other solutions have been proposed and tested, such as chitosan (CH) (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Chelating Agents Used In Endodontics and Their Influence on MMPs of Cell Membranes

Pivatto¹,

Pedro²,

Guedes³

et al. 2020

Braz. Dent. J.

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This study evaluated the cytotoxic effect and the ability to inhibit matrix metalloproteinases (MMP-2 and MMP-9) of 0.2% chitosan (CH) and 1% acetic acid (AA) compared with 17% ethylenediaminetetraacetic acid (EDTA). Cell viability assay was performed according to ISO 10993-5 with mouse fibroblasts (L929). The culture was exposed to 0.2% CH, 1% AA, and 17% EDTA. The chelating agents were evaluated immediately after contact with the cells and after 6 h, 12 h, and 24 h of incubation. Cell viability was analyzed using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Inhibition of the gelatinolytic activity of MMP-2 and MMP-9 was evaluated by gelatin zymography. Different concentrations of CH were evaluated: 50 mM, 5 mM, 0.5 mM, and 0.05 mM. EDTA (0.5 mM) was used as a positive control. The results demonstrated that CH and AA had an initial cytotoxic effect, which decreased after 6 h, 12 h, and 24 h, being statistically similar to EDTA (P > 0.05). Additionally, CH at concentrations of 50 mM, 5 mM, and 0.5 mM had an inhibitory effect on MMP-2 and MMP-9, similar to that of the control with EDTA. The chelating agents had no cytotoxic effects after 24 h. MMP-2 and MMP-9 were inhibited by the experimental solutions.

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Effect of chitosan nanoparticle, QMix, and EDTA on TotalFill BC sealers’ dentinal tubule penetration: a confocal laser scanning microscopy study

Cited by 33 publications

References 36 publications

Effectiveness of Various Final Irrigation Techniques on Sealer Penetration in Curved Roots: A Confocal Laser Scanning Microscopy Study

Effectiveness of Various Final Irrigation Techniques on Sealer Penetration in Curved Roots: A Confocal Laser Scanning Microscopy Study

The effect of 17% EDTA and QMiX ultrasonic activation on smear layer removal and sealer penetration: ex vivo study

Cytotoxicity of Chelating Agents Used In Endodontics and Their Influence on MMPs of Cell Membranes

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