Effect of calcium salts on Allium cepa chromosomes.

Misra, M. P.

doi:10.1508/cytologia.47.47

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…Stickiness and clumping of the chromosomes were some of the most common effects of these extracts on the treated root tips. These abnormalities have also been reported for several extracts and chemicals already investigated (Badr and Elkington 1982, Misra 1982, Nwakanma et al 2009). Stickiness usually leads to the formation of anaphase and telophase bridges and this end up inhibiting metaphase and cytokinesis respectively and thus hampering cell division.…”

Section: Discussionsupporting

confidence: 67%

Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus

Nwakanma¹,

Okoli²

2010

EJOBIOS

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The mitotic effects of the root extracts of Boerhaavia diffusa on the root tips of Crinum jagus were investigated. The results of this study showed several chromosomal abnormalities including stickiness of chromosomes (both at metaphase and anaphase), C-metaphase, lagging chromosomes, and sticky bridges. The trend of the results showed that the higher the concentration of the extracts for treatment, the more inhibitory the effect on mitosis with more pronounced chromosomal aberrations. The root extract of B. diffusa was found active at concentrations of 0.1%, 0.2%, 0.4%, and 0.8%. The extract was found to accumulate metaphase and could thus be of immense help in cytological work. These results are discussed in the light of their reported use in cancer therapy, the treatment of other diseases in traditional medicinal practice and its' possible use as an alternative to colchicine in cytological work.Nwakanma NMC, Okoli BE (2010) Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus. EurAsia J BioSci 4: 105-111.

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Section: Discussionsupporting

confidence: 67%

Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus

Nwakanma¹,

Okoli²

2010

EJOBIOS

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“…Singh and Kashyap (1978) in their studies on the effects of manganese chloride in blue green algae observed that the chemical induced asymmetric cell divisions. Studies using calcium salt in Allium Lepa showed that calcium ion concentration in cells played important roles in bringing about chromosome brakage, stickiness and other abnormalities (Misra 1982). Steffensen (1953Steffensen ( , 1955 observed that spontaneous chromosome breakage is greatly increased when calcium or magnesium is deficient in the culture medium in which the plants are grown.…”

Section: Resultsmentioning

confidence: 99%

Production of mitotic abnormalities by magnesium sulphate in Vicia faba L.

Abraham

Nair

1989

CYTOLOGIA

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“…The observations showed that fertilizers like ammonium sulfate, ammonium phosphate, ammonium nitrate and potassium chloride produced chromosome breakage and other abnormalities during cell division in Allium cepa (Abraham 1965). Studies using calcium salt in Allium cepa showed that calcium ion concentration in cells played important roles in bringing about chromosome breakage, stickiness and other abnormalities (Misra 1982).…”

Section: Cyto-genotoxicity Of Fertilizers Monitored By Allium Root Timentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student's t, and the nonparametric tests Kruskal-Wallis and Mann-Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.

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Effect of calcium salts on Allium cepa chromosomes.

Abstract: Interesting results have been obtained by exposing chromosomes to various chemicals and physical factors

Cited by 3 publications

References 3 publications

Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus

Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus

Production of mitotic abnormalities by magnesium sulphate in Vicia faba L.

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

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