The aim of this work was to evaluate the potential of cellulose nanofibrils (CNFs) films treated with commercial solution of Ag nanoparticles stabilized with natural tannin and silane groups in the bacteria growth inhibition. The films were produced with commercial pulps of pine and eucalyptus treated with sodium hydroxide (5% w/v) and calcium hydroxide (10% w/v), respectively. The bacteria selected for the inhibition test were the gram-positive Listeria monocytogenes and gram-negative Salmonella enteriditis. The films performance was investigated by the antimicrobial activity using disc diffusion. In addition, the pulps were evaluated by their chemical composition, water retention index (WRI) and X-ray diffraction (XRD). The content of hemicelluloses in the treated pine pulp (11.32%) was lower in comparison to the found in the treated eucalyptus pulp (14.46%). The XRD diffractogram presented higher crystallinity index for the treated eucalyptus pulp (70%) with a characteristic peak of calcium carbonate. The treated eucalyptus pulp showed higher WRI and viscosity values in relation to the treated pine pulp. Growth inhibitory halos were more expressive when the treatment with both antimicrobial solutions were proceeded in the films produced with pine CNFs with addition of the citric acid. The effect of the nanoparticles stabilized with tannin must be highlighted, mainly on the bacterium S. enteriditis.