Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in &lt;i&gt;Dendrobium&lt;/i&gt; sp. orchid

Islam, M. O.; Islam, M. S.; Saleh, Abu

doi:10.3329/agric.v13i1.26553

Cited by 14 publications

(14 citation statements)

References 7 publications

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“…Islam et al [46] investigated the effects of different BH concentrations (0, 2.5, 5, 10, and 20%; w/v) on the growth and development of PLBs Dendrobium sp. and showed that ½ MS medium supplemented with 10% BH was the most suitable for PLBs regeneration.…”

Section: Banana Homogenatementioning

confidence: 99%

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Utami

Hariyanto

2020

International Journal of Agronomy

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In nature, orchid seed germination is obligatory following infection by mycorrhizal fungi, which supplies the developing embryo with water, carbohydrates, vitamins, and minerals, causing the seeds to germinate relatively slowly and at a low germination rate. The nonsymbiotic germination of orchid seeds found in 1922 is applicable to in vitro propagation. The success of seed germination in vitro is influenced by supplementation with organic compounds. Here, we review the scientific literature in terms of the contents and role of organic supplements in promoting seed germination, protocorm development, and seedling growth in orchids. We systematically collected information from scientific literature databases including Scopus, Google Scholar, and ProQuest, as well as published books and conference proceedings. Various organic compounds, i.e., coconut water (CW), peptone (P), banana homogenate (BH), potato homogenate (PH), chitosan (CHT), tomato juice (TJ), and yeast extract (YE), can promote seed germination and growth and development of various orchids. They also stimulate seedling development, formation of protocorm-like bodies (PLBs), plantlet growth, and multiple shoot formation. The addition of organic compounds to culture media, individually or in combination, accelerates seed germination and seedling development. Different types and concentrations of organic nutrients are needed for the success of in vitro cultures, depending on the species and genotype.

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Section: Banana Homogenatementioning

confidence: 99%

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Utami

Hariyanto

2020

International Journal of Agronomy

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“…For number of leaves and roots, the interaction culture medium x irradiance showed higher values in the combination B100 + 3,000 K-1, averaging 15.75 leaves and 12.83 roots. When studying the in vitro culture of Dendrobium sp., Islam et al (2015) observed that the use of 100 g L -1 or 200 g L -1 of banana decreased the number of leaves, in comparison to the media without the additive (Figure 1).…”

Section: Pseudobulb Diametermentioning

confidence: 91%

In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

Freitas

Sorgato

Soares

et al. 2021

Pesqui. Agropecu. Trop.

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Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

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“…Banyaknya daun yang terbentuk ini terjadi karena peran auksin (air kelapa) yang mampu menambah jumlah daun (Nuraini et al, 2010). Gambar 7 menunjukkan planlet anggrek Phalaenopsis yang selalu membentuk PLB baru dapat diduga karena selalu mendapatkan nutrisi sehingga masih mempunyai kemampuan untuk tumbuh dengan baik pada posisi di pangkal perakaran anggrek seperti yang dilaporkan oleh Islam et al (2015).…”

Section: Regenerasi Dan Aklimatisasi Anggrekunclassified

Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid

Restanto¹,

Kriswanto²,

Iqmatullah³

et al. 2021

Agrikultura

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Phalaenopsis merupakan anggrek yang mempunyai nilai ekonomis tinggi karena warna yang menarik. Permintaan selalu meningkat sehingga tehnologi kultur jaringan sangat dibutuhkan dalam perbanyakan anggrek ini. Perbanyakan tanaman anggrek menggunakan sumber eksplan protocorm-like body (PLB) merupakan salah satu cara yang tepat untuk menghasilkan anggrek dalam jumlah banyak. Penelitian ini bertujuan untuk mendapatkan kombinasi NAA dan Kinetin terbaik pada perkembangan PLB di kultur cair serta PLB yang disubkultur di media regenerasi. Bahan tanam menggunakan PLB umur 2 bulan yang ditanam pada media Vacin & Went (VW) cair dengan perlakuan kombinasi NAA dan Kinetin masing-masing pada konsentrasi 2,5 mg/L, 5 mg/L dan 7,5 mg/L. Media regenerasi menggunakan VW padat dengan suplemen 15% air kelapa, 5% pisang, 5% kentang dan 0,2% arang aktif. Percobaan menggunakan Rancangan Acak Lengkap dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa kombinasi NAA dan kinetin tidak berpengaruh terhadap jumlah dan berat PLB. Sementara itu, warna PLB hampir semua menunjukkan warna yang sama yaitu hijau kekuningan (5GY 7/6) hanya pada kombinasi perlakuan NAA 5 mg/L + Kinetin 7,5 mg/L terjadi klorosis berwarna kuning pucat (5Y8/4). Perubahan warna planlet terjadi dari hijau kekuningan terang (7.5GY8/8) menjadi hijau kekuningan (7.5GY7/6) dan hijau kekuningan tua (7.5GY6/10) masing-masing pada awal aklimatisasi, serta pada dua dan empat minggu. Bibit anggrek tumbuh dengan baik dan menjadi tanaman dewasa setelah dipindahkan pada media moss hitam.

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Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in <i>Dendrobium</i> sp. orchid

Cited by 14 publications

References 7 publications

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid

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