Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid

Restanto, Didik Pudji; Kriswanto, Budi; Iqmatullah, Nafisah; Dewanti, Parawita

doi:10.24198/agrikultura.v32i2.32095

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Keterlambatan perkembangan anggrek di Indonesia karena tidak ada sentuhan teknologi terutama inovasi teknologi klonal. Hasil penelitian Restanto et al (2021) tentang penggunaan hormon Naphthalene Acetic Acid (NAA) dan Kinetin terhadap perkembangan Protocorm-Like Body (PLB) dan Regenerasi pada Anggrek Phalaenopsis sp. hybrid dari biji.…”

Section: Pendahuluanunclassified

PELATIHAN AKLIMATISASI ANGGREK Dendrobium BAGI REMAJA MUSALA DARUL IMAN KECAMATAN SUMBERSARI JEMBER

Restanto,

Dewanti,

Handoyo

et al. 2024

papuma

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The Dendrobium sp orchid is an orchid that is suitable for living in equatorial areas because it requires full light. Acclimatization is a very important stage for future orchid development. The aim of this service is to provide training to member of the Musala Darul Iman to understand orchid acclimatization techniques which can be used as equipment in orchid agribusiness. From the results of this trial, the audience's enthusiasm was obtained in listening to the lecture and practice, both in cultivation and agribusiness. Seedlings to be acclimatized should be healthy and perfect, good technique of transferring to a soft pot must be solid, watering was done after 7 days to give the root system the opportunity to develop well and required high levels of patience.

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Section: Pendahuluanunclassified

PELATIHAN AKLIMATISASI ANGGREK Dendrobium BAGI REMAJA MUSALA DARUL IMAN KECAMATAN SUMBERSARI JEMBER

Restanto,

Dewanti,

Handoyo

et al. 2024

papuma

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“…This process is initiated by growth hormones, namely auxins and cytokinins. The composition between auxin and cytokinin hormones can cause the growth of Protocorm-Like Bodies (PLB) with the aim of research [12]. Induction of Protocorm-Like Bodies (PLB) from several orchid explants such as pieces of leaves, stems, root tips and seeds in various compositions of ZPT (Growth Regulatory Substances) and media is not easy, and the same thing was also reported by Hardjo et al (2016) who successfully induced PLB from stem and leaf explants of Vanda tricolor Lindl.var pallida using NAA 1.0 ppm + BAP 0.5 ppm after 4 weeks of culture, but there is no data on PLB regeneration.…”

Section: Introductionmentioning

confidence: 99%

The Effect of NAA and BAP in Induction of Protocorm Like Bodies (PLB) Cattleya sp. Orchid In Vitro

Siregar,

Harahap,

Idramsa

et al. 2023

Proceedings of the 8th Annual International Seminar on Transformative Education and Educational Leadership, AISTEEL 2023, 19 Se

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Cattleya sp. seeds do not have an endosperm that cause orchid is threatened with extinction. Forming Protocorm-Like Bodies (PLB) that capable of producing large quantities of orchid seeds quickly. The aim was to determine the best interaction of NAA and BAP on growth induction of PLB. The research method used random design with NAA: 0; 0.5; 1; 1.5 ppm and BAP: 0; 0,5; 1 ppm. The data were analyzed with ANAVA and using the colorgrab application. The result showed there is significantly effect of NAA and BAP interaction on the time PLB was formed and there is no significant interaction on the number of PLB. PLB formed the fastest in week 3 with MS + NAA 1.5 ppm + BAP 1 ppm, the highest number of PLB was found in MS + NAA 1.5 ppm + BAP 1 ppm which was 6,67 PLB. The best color of PLB being green.

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Induksi Somatic Embriogenesis dan Kultur Suspensi Sel Pada Tanaman Porang (Amorphophallus muelleri Blume)

Restanto

Farlisa²,

Dewanti³

et al. 2022

Agriprima

Self Cite

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Porang adalah tanaman yang tumbuh di daerah tropis dibawah tegakan hutan. Porang termasuk tanaman komersial banyak diminati oleh masyarakat karena mengandung glukomanan yang cukup tinggi. Kebutuhan bibit melalui katak dan umbi relative mahal dalam budidaya porang sehingga dengan pendekatan kultur jaringan melalui Somatic Embryogenesis (SE) dan suspensi sel untuk perbanyakan masal bibit porang sangat memungkinkan. Hasil SE digunakan untuk kultur suspensi sel agar menghasilkan bibit dalam jumlah banyak. Penelitian ini bertujuan untuk menghasilkan SE dalam jumlah banyak sebagai bahan kultur suspensi sel. Perbanyakan SE menggunakan Rancangan Acak lengkap (RAL) factorial, factor pertama konsentrasi NAA dengan konsentrasi 0,5 ppm, 1 ppm, dan 1,5 ppm. Factor kedua konsentrasi 2,4-D konsentrasi 1 ppm dan 2 ppm sehingga terdapat 6 kombinasi perlakuan diulang sebanyak 4 kali. Hasil SE terbaik selanjutnya dikultur suspensi sel menggunakan hormon NAA 0,25 ml di-shaker selama 8 minggu diinkubasi pada kondisi gelap. Parameter pengamatan terdiri dari kedinian munculnya kalus, persentase kalus, struktur, warna kalus, proliferasi kalus, histologi kalus, respon hasil suspensi, proliferasi kalus hasil kultur suspensi. Data dianalisis menggunakan DMRT pada taraf 5%, sedangkan parameter suspensi sel dianalisis secara deskriptif. Hasil penelitian SE terbaik pada perlakuan kombinasi 1 ppm NAA + 2 ppm 2,4-D menghasilkan persentase kalus tertinggi yaitu 90%, warna kalus dengan skoring 5Y 8/6 berwarna putih susu yang remah. Hasil kultur suspensi menggunakan hormon NAA dengan konsesntrasi 0,25 ppm menunjukkan pertumbuhan kalus tertinggi yaitu dengan menghitung volume endapan kalus terjadi pada fase eksponensial (7 minggu inkubasi) mencapai 3,67 ml.

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Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid

Cited by 3 publications

References 8 publications

PELATIHAN AKLIMATISASI ANGGREK Dendrobium BAGI REMAJA MUSALA DARUL IMAN KECAMATAN SUMBERSARI JEMBER

PELATIHAN AKLIMATISASI ANGGREK Dendrobium BAGI REMAJA MUSALA DARUL IMAN KECAMATAN SUMBERSARI JEMBER

The Effect of NAA and BAP in Induction of Protocorm Like Bodies (PLB) Cattleya sp. Orchid In Vitro

Induksi Somatic Embriogenesis dan Kultur Suspensi Sel Pada Tanaman Porang (Amorphophallus muelleri Blume)

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