Effect of Bacillus-based direct-fed microbials on Eimeria maxima infection in broiler chickens

Lee, Kyung-Woo; Lillehoj, Hyun S.; Jang, Seung I.; Li, Guangxing; Lee, Sung-Hyen; Lillehoj, Erik P.; Siragusa, Gregory R.

doi:10.1016/j.cimid.2010.06.001

Cited by 73 publications

(44 citation statements)

References 20 publications

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“…While present in the challenge inoculum, the dose of E. tenella may not have been sufficient to cause extensive damage to the site of infection, resulting in the absence of lesions in the ceca. Similar to present results, Lee et al (2010) reported that birds given a strain of a Bacillus-based direct-fed microbial had significantly lower lesions scores in the gastrointestinal tract than birds given the nonsupplemented diet following an E. maxima challenge. Studies investigating necrotic enteritis in broilers found birds given 2 different blends of direct-fed microbials had significantly reduced intestinal lesions due to necrotic enteritis than birds in the positive control (McReynolds et al, 2009).…”

Section: Discussionsupporting

confidence: 93%

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Ritzi

Abdelrahman

Mohnl³

et al. 2014

Poultry Science

128

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Coccidiosis is an inherent risk in the commercial broiler industry and inflicts devastating economic losses to poultry operations. Probiotics may provide a potential alternative to the prophylactic use of anticoccidials in commercial production. This study evaluated the effects of probiotic applications (feed and water) on bird performance and resistance to a mixed Eimeria infection in commercial broilers. On day of hatch, 1,008 commercial male broilers (Cobb 500) were assigned to 1 of 6 treatments (8 replicate floor pens; 21 birds/pen), including noninfected negative control (NEG), Eimeria-infected positive control (POS), anticoccidial control (0.01% salinomycin, SAL), intermittent high-dose water-applied probiotic (WPI), continuous low-dose water-applied probiotic (WPC), and feed-supplemented probiotic (FSP). On d 15, all birds except those in NEG were challenged with a mixed inoculum of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Measurements were taken on d 7, 15, 21, 28, 35, and 42. Fecal samples were collected from d 20 to 24 for oocyst counts, and lesion scores were evaluated on d 21. Data were analyzed using the Fit Model platform in JMP Pro 10.0 (SAS Institute Inc.). Differences in experimental treatments were tested using Tukey's honestly significant difference following ANOVA with significance reported at P ≤ 0.05. Overall, NEG birds outperformed all other groups. For performance, the probiotic groups were comparable with the SAL-treated birds, except during the 6 d immediately following the Eimeria species challenge, where the SAL birds exhibited better performance. The WPC birds had lower duodenal and jejunal lesion scores, indicating a healthier intestine and enhanced resistance to Eimeria species compared with POS. Birds in the WPI treatment shed fewer oocysts in the feces, although this was not a trend for all of the probiotic treatment groups. The results of this study suggest probiotic supplementation without anticoccidials can enhance performance and help alleviate the negative effects of a mixed Eimeria infection.

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Section: Discussionsupporting

confidence: 93%

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Ritzi

Abdelrahman

Mohnl³

et al. 2014

Poultry Science

128

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“…It is well documented that dietary DFMs modulate various aspects of immunity in broiler chickens (Lee et al, 2010a,b,c). Also, we have recently reported that the exposure of growing chickens to used poultry litter stimulated humoral and cell-mediated immune responses, presumably due to contact with contaminating enteric pathogens (Lee et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In our laboratory, DFM-based immunomodulation as an alternative coccidiosis control strategy has been documented (Lee et al, 2010c). For example, it has been shown that dietary Bacillus subtilis -based DFMs reduced the clinical signs of experimental avian coccidiosis with concomitant increase in protective immunity in broiler chickens (Lee et al, 2010a). In addition, Bacillus -based DFMs modulated various parameters of inflammation, and humoral and cellular immunities in broiler chickens (Lee et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Effect of <italic>Bacillus Subtilis</italic>-based Direct-fed Microbials on Immune Status in Broiler Chickens Raised on Fresh or Used Litter

Lee

Lillehoj

Jang

et al. 2013

Asian Australas. J. Anim. Sci

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Type of dietary direct-fed microbials (DFMs) or poultry litter could directly influence the composition of gut microbiota. Gut microbiota plays an important role in shaping the developing immune system and maintaining the homeostasis of the mature immune system in mammal and chickens. The present study was carried out to investigate the interaction among litter, DFMs and immunity in broiler chickens exposed to a field-simulated environment. Immune status of broiler chickens was assessed by serum antibodies against Eimeria spp. and Clostridium spp. and intestinal cytokine mRNA expression. The current experimental design had a 3 ×2 factorial arrangement of treatments with three types of litter, i.e., fresh litter or used litter that was obtained from a farm with no disease outbreak (used litter) or a farm with history of a gangrenous dermatitis outbreak (GD litter), and two dietary treatments with or without DFMs. It was found that either DFM addition or type of litter significantly affected anticoccidial antibody levels of broiler chickens at d 42. In general, dietary DFMs increased the anticoccidial antibodies in the fresh-litter raised chickens, but lowered the levels in the GD-litter raised chickens. Serum antibodies against Clostridium perfringens α-toxin were significantly (p<0.05) higher in chickens raised on GD litter compared with those raised on fresh litter. Cytokine mRNA expression was significantly (p<0.05) altered by either the type of litter or DFMs. Of interest, dietary DFMs lowered interferon-γ, interleukin 1beta, and CXCLi2 cytokine mRNA expression in chickens raised on fresh litter but increased them in GD-litter raised chickens. In conclusion, dietary DFMs modulate various immune parameters of broiler chickens, but the DFM-mediated effects were dependent upon the type of litter on which chickens were raised.

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“…However, the response of broilers in T1 and T2 groups was similar to that described by Revolledo et al (2009), who demonstrated that a mixture of Lactobacillus, Enterococcus, and Bifidobacteria could activate the levels of total IgA in the intestinal fluid after infection with S. typhimurium. Lee et al (2010) have also reported that dietary B. subtilis-based probiotics could reduce the clinical symptoms of coccidiosis and enhance immunity of broilers against challenges with Eimeria maxima. In addition, De Simone et al (1993) and Miettinen et al (1996) has reported that the addition of probiotics can increase immune cell proliferation and diminish the production of proinflammatory cytokines such as TNF-α and IL-6.…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietary <i>Bacillus Subtilis </i>C14 and RX7 Strains on Growth Performance, Blood Parameter, and Intestinal Microbiota in Broiler Chickens Challenged with <i>Salmonella Gallinarum</i>

et al. 2017

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Sixty broilers (initially 1.6 kg and 35 d-old) were used to determine the effect of Bacillus subtilis C14 and RX7 strains on growth performance, blood parameter, and intestinal microbiota in response to experimental challenge with Salmonell gallinarum. Broilers were distributed to 4 treatment groups include: C1 (control group; no challenge, no B. subtilis), C2 (Salmonella-challenged group; S. gallinarum 10 8 cfu/bird), T1 (C2+supplemented with of B. subtilis C14 (1.0×10 9 cfu/g) at 0.1% in diet) and T2 (C2+supplemented with of B. subtilis RX7 (1.0×10 9 cfu/g) at 0.1% in diet). Results indicated that inclusion of B. subtilis (T1, T2) in the diet increased (P＜0.05) the weight gain and feed intake, and improved feed conversion of challenged broilers compared with no B. subtilis supplementation diet (C2). Improvements (P＜0.05) in the immunoglobulin A concentration were observed by the addition of B. subtilis compared with C2 treatment, whereas tumor necrosis factor-α was decreased (P＜0.05). Latobacillus number in small and large intestines was higher (P＜0.05) by B. subtilis additon than C2 treatment but Salmonella numbers were lower (P＜0.05). The results suggested that dietary supplementation of B. subtilis C14 and RX7 improved the growth performance, and affected the blood profiles and intestinal microbiota of broilers against S. gallinarum infection. Therefore, B. subtilis C14 and RX7 may have beneficial effects, in relieving the stress of broilers infected with S. gallinarum.

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Effect of Bacillus-based direct-fed microbials on Eimeria maxima infection in broiler chickens

Cited by 73 publications

References 20 publications

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Effect of <italic>Bacillus Subtilis</italic>-based Direct-fed Microbials on Immune Status in Broiler Chickens Raised on Fresh or Used Litter

Effect of Dietary <i>Bacillus Subtilis </i>C14 and RX7 Strains on Growth Performance, Blood Parameter, and Intestinal Microbiota in Broiler Chickens Challenged with <i>Salmonella Gallinarum</i>

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Effect of Bacillus-based direct-fed microbials on Eimeria maxima infection in broiler chickens

Cited by 73 publications

References 20 publications

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge

Effect of &lt;italic&gt;Bacillus Subtilis&lt;/italic&gt;-based Direct-fed Microbials on Immune Status in Broiler Chickens Raised on Fresh or Used Litter

Effect of Dietary <i>Bacillus Subtilis </i>C14 and RX7 Strains on Growth Performance, Blood Parameter, and Intestinal Microbiota in Broiler Chickens Challenged with <i>Salmonella Gallinarum</i>

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Effect of <italic>Bacillus Subtilis</italic>-based Direct-fed Microbials on Immune Status in Broiler Chickens Raised on Fresh or Used Litter